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1Protein production in Escherichia coli is guided by the trade-off between intracellular substrate availability and energy cost
A. Niess, M. Siemann-Herzberg, and R. Takors. Microbial Cell Factory, (2019)
6 years ago by @siemannherzberg
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myown
proteinsynthesis
myownproteinsynthesis
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5Cell-Free Protein Synthesis From Fast-Growing Vibrio natriegens
J. Failmezger, S. Scholz, B. Blombach, and M. Siemann-Herzberg. Frontiers in Microbiology, (June 2018)
7 years ago by @siemannherzberg
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myown
proteinsynthesis
myownproteinsynthesis
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5Cell-free protein synthesis from non-growing, stressed Escherichia coli
J. Failmezger, M. Rauter, R. Nitschel, M. Kraml, and M. Siemann-Herzberg. SCIENTIFIC REPORTS, (November 2017)
7 years ago by @siemannherzberg
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myown
proteinsynthesis
myownproteinsynthesis
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1Kinetic modeling and simulation of in vitro transcription by phage T7 RNA polymerase
S. Arnold, M. Siemann, K. Scharnweber, M. Werner, S. Baumann, and M. Reuss. BIOTECHNOLOGY AND BIOENGINEERING, 72 (5): 548-561 (March 2001)
7 years ago by @siemannherzberg
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myown
proteinsynthesis
myownproteinsynthesis
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1Quantifying ribosome dynamics in Escherichia coli using fluorescence
J. Failmezger, J. Ludwig, A. Niess, and M. Siemann-Herzberg. FEMS MICROBIOLOGY LETTERS, (March 2017)
7 years ago by @siemannherzberg
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myown
proteinsynthesis
myownproteinsynthesis
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3Experimentally Validated Model Enables Debottlenecking of in Vitro Protein Synthesis and Identifies a Control Shift under in Vivo Conditions
A. Niess, J. Failmezger, M. Kuschel, M. Siemann-Herzberg, and R. Takors. ACS SYNTHETIC BIOLOGY, 6 (10): 1913-1921 (October 2017)
7 years ago by @siemannherzberg
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myown
proteinsynthesis
myownproteinsynthesis
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1In vitro coupled transcription translation: Effects of modification in lysate preparation on protein composition and biosynthesis activity
P. Schindler, S. Baumann, M. Reuss, and M. Siemann. ELECTROPHORESIS, 21 (13): 2606-2609 (July 2000)Meeting of the German-Electrophoresis-Society, MUNICH, GERMANY, OCT 25-27, 1999.
7 years ago by @siemannherzberg
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myown
proteinsynthesis
myownproteinsynthesis
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1Investigation of translation dynamics under cell-free protein biosynthesis conditions using high-resolution two-dimensional gel electrophoresis
P. Schindler, F. Macherhammer, S. Arnold, M. Reuss, and M. Siemann. ELECTROPHORESIS, 20 (4-5): 806-812 (April 1999)3rd Siena 2-D Electrophoresis Meeting - From Genome to Proteome, SIENA, ITALY, AUG 31-SEP 03, 1998.
7 years ago by @siemannherzberg
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myown
proteinsynthesis
myownproteinsynthesis
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1Formation of bacteriophage MS2 infectious units in a cell-free translation system
V. Katanaev, A. Spirin, M. Reuss, and M. Siemann. FEBS LETTERS, 397 (2-3): 143-148 (November 1996)
7 years ago by @siemannherzberg
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myown
protein
proteinsynthesis
myownproteinproteinsynthesis
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1Site-Specific Cleavage of Ribosomal RNA in Escherichia coli-Based Cell-Free Protein Synthesis Systems
J. Failmezger, R. Nitschel, A. Sanchez-Kopper, M. Kraml, and M. Siemann-Herzberg. PLoS One, (December 2016)
7 years ago by @siemannherzberg
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imported
myown
proteinsynthesis
importedmyownproteinsynthesis
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3Identification of factors impeding the production of a single-chain antibody fragment in Escherichia coli by comparing in vivo and in vitro expression
P. Oelschlaeger, S. Lange, J. Schmitt, M. Siemann, M. Reuss, and R. Schmid. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 61 (2): 123-132 (April 2003)
7 years ago by @siemannherzberg
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imported
myown
proteinsynthesis
importedmyownproteinsynthesis
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1Hydantoinases and related enzymes as biocatalysts for the synthesis of unnatural chiral amino acids
J. Altenbuchner, M. Siemann-Herzberg, and C. Syldatk. CURRENT OPINION IN BIOTECHNOLOGY, 12 (6): 559-563 (December 2001)
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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2Teaching bioprocess engineering to undergraduates: Multidisciplinary hands-on training in a one-week practical course
M. Henkel, M. Zwick, J. Beuker, J. Willenbacher, S. Baumann, F. Oswald, A. Neumann, M. Siemann-Herzberg, C. Syldatk, and R. Hausmann. BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION, 43 (3): 189-202 (May 2015)
7 years ago by @siemannherzberg
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bioprocess
biotechnology;
engineering;
exercise}
laboratory
myown
{bioreactor;
bioprocessbiotechnology;engineering;exercise}laboratorymyown{bioreactor;
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3Applying systems biology tools to study n-butanol degradation in Pseudomonas putida KT2440
T. Vallon, O. Simon, B. Rendgen-Heugle, S. Frana, B. Mueckschel, A. Broicher, M. Siemann-Herzberg, J. Pfannenstiel, B. Hauer, A. Huber and 2 other author(s). ENGINEERING IN LIFE SCIENCES, 15 (8): 760-771 (November 2015)
7 years ago by @siemannherzberg
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Butanol;
Chemostat
Omics;
Stress
culture;
myown
response}
{Biofuels;
Butanol;ChemostatOmics;Stressculture;myownresponse}{Biofuels;
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2Evolution of pyruvate kinase-deficient Escherichia coli mutants enables glycerol-based cell growth and succinate production
S. Soellner, M. Rahnert, M. Siemann-Herzberg, R. Takors, and J. Altenbuchner. JOURNAL OF APPLIED MICROBIOLOGY, 115 (6): 1368-1378 (December 2013)
7 years ago by @siemannherzberg
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POMP
carboxylase;
deletion;
evolution;
gene
kinase}
metabolic
myown
pathway;
phosphoenolpyruvate
pyruvate
rerouting;
{flux
POMPcarboxylase;deletion;evolution;genekinase}metabolicmyownpathway;phosphoenolpyruvatepyruvatererouting;{flux
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1Global Transcription and Metabolic Flux Analysis of Escherichia coli in Glucose-Limited Fed-Batch Cultivations
K. Lemuth, T. Hardiman, S. Winter, D. Pfeiffer, M. Keller, S. Lange, M. Reuss, R. Schmid, and M. Siemann-Herzberg. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 74 (22): 7002-7015 (November 2008)
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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1A new method for the detection of hydantoinases with respect to their enantioselectivity on acrylamide gels based on enzyme activity stain
O. May, M. Siemann, and C. Syldatk. BIOTECHNOLOGY TECHNIQUES, 12 (4): 309-312 (April 1998)
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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1Crystallization and preliminary X-ray analysis of a hydantoinase from Arthrobacter aurescens DSM 3745
O. May, M. Siemann, C. Syldatk, K. Niefind, and D. Schomburg. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 52 (6): 1209-1210 (November 1996)
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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1DETECTION AND COMPARISON OF STRAINS WITH SELECTIVE L-HYDANTOIN CLEAVING ACTIVITY USING POLYCLONAL ANTIBODIES
M. SIEMANN, C. SYLDATK, and F. WAGNER. BIOTECHNOLOGY TECHNIQUES, 7 (5): 361-366 (May 1993)
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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1Protein purification using magnetic adsorbent particles
M. Franzreb, M. Siemann-Herzberg, T. Hobley, and O. Thomas. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 70 (5): 505-516 (May 2006)
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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1ENHANCED STABILITY OF AN L-HYDANTOINASE MEDIATED BY ITS CORRESPONDING POLYCLONAL ANTIBODY
M. SIEMANN, C. SYLDATK, and F. WAGNER. BIOTECHNOLOGY LETTERS, 16 (4): 349-354 (April 1994)
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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1Prediction of kinetic parameters from DNA-binding site sequences for modeling global transcription dynamics in Escherichia coli
T. Hardiman, H. Meinhold, J. Hofmann, J. Ewald, M. Siemann-Herzberg, and M. Reuss. METABOLIC ENGINEERING, 12 (3): 196-211 (May 2010)
7 years ago by @siemannherzberg
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1,6-bis(phosphate);
Central
Cra;
Fed-batch;
Fructose
Gene
Glucose-limitation;
Growth
Position
Promoter;
RNA
Regulation;
Transcription
carbon
fruR;
matrix;
metabolism;
myown
network;
polymerase;
rate-dependent
rate}
regulation;
regulatory
volume;
weight
{Cell
1,6-bis(phosphate);CentralCra;Fed-batch;FructoseGeneGlucose-limitation;GrowthPositionPromoter;RNARegulation;TranscriptioncarbonfruR;matrix;metabolism;myownnetwork;polymerase;rate-dependentrate}regulation;regulatoryvolume;weight{Cell
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1Quantitative profiling of the phosphotransferase system in Escherichia coli
G. Franke, N. Stoepel, K. Reidegeld, M. Siemann-Herzberg, M. Reuss, H. Meyer, and B. Warscheid. MOLECULAR & CELLULAR PROTEOMICS, 4 (8, 1): S319 (August 2005)
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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1In vitro synthesis and characterization of guanosine 3 `,5 `-bis(diphosphate)
T. Hardiman, V. Windeisen, J. Ewald, S. Zibek, P. Schlack, J. Rebell, M. Reuss, and M. Siemann-Herzberg. ANALYTICAL BIOCHEMISTRY, 383 (2): 337-339 (December 2008)
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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1Quantifying cytosolic messenger RNA concentrations in Escherichia coli using real-time polymerase chain reaction for a systems biology approach
T. Schuhmacher, K. Lemuth, T. Hardiman, G. Vacun, M. Reuss, and M. Siemann-Herzberg. ANALYTICAL BIOCHEMISTRY, 398 (2): 212-217 (March 2010)
7 years ago by @siemannherzberg
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Absolute
Gene
Internal
concentrations;
expression}
mRNA
myown
qRT-PCR;
quantification;
standard;
{Cytosolic
AbsoluteGeneInternalconcentrations;expression}mRNAmyownqRT-PCR;quantification;standard;{Cytosolic
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1Direct bioproduct isolation from crude feedstocks using magnetic micro adsorbents
M. Franzreb, N. Ebner, M. Siemann, J. Altenbuchner, C. Fritz, Z. Mayer, N. Schultz, and C. Syldakt. JOURNAL OF BIOTECHNOLOGY, 118 (1): S53 (August 2005)12th European Congress on Biotechnology (ECB 12), Copenhagen, DENMARK, AUG 21-24, 2005.
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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1A new method for determining delta C-13 and delta D simultaneously for CH4 by gas chromatography continuous-flow isotope-ratio mass spectrometry
J. Potter, and M. Siemann. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 18 (2): 175-180 (2004)
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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1Integrated multilaboratory systems biology reveals differences in protein metabolism between two reference yeast strains
A. Canelas, N. Harrison, A. Fazio, J. Zhang, J. Pitkanen, J. van den Brink, B. Bakker, L. Bogner, J. Bouwman, J. Castrillo and 25 other author(s). NATURE COMMUNICATIONS, (December 2010)
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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1Model-based inference of gene expression dynamics from sequence information
S. Arnold, M. Siemann-Herzberg, J. Schmid, and M. Reuss. BIOTECHNOLOGY FOR THE FUTURE, volume 100 of Advances in Biochemical Engineering-Biotechnology, SPRINGER-VERLAG BERLIN, HEIDELBERGER PLATZ 3, D-14197 BERLIN, GERMANY, (2005)
7 years ago by @siemannherzberg
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and
biosynthesis;
degradation}
mRNA
modeling
myown
protein
simulation;
transcription;
translation;
{dynamic
andbiosynthesis;degradation}mRNAmodelingmyownproteinsimulation;transcription;translation;{dynamic
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1Cloning of D-specific hydantoin utilization genes from Arthrobacter crystallopoietes
M. Werner, F. Vazques, C. Fritz, O. Vielhauer, M. Siemann-Herzberg, J. Altenbuchner, and C. Syldatk. ENGINEERING IN LIFE SCIENCES, 4 (6): 563-572 (October 2004)Symposium Degussa Bio-Tech Days, Hanau Wolfgang, GERMANY, MAR 22-23, 2004.
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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1Microbial hydantoinases - industrial enzymes from the origin of life?
C. Syldatk, O. May, J. Altenbuchner, R. Mattes, and M. Siemann. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 51 (3): 293-309 (March 1999)
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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1Separation and detection of macrolide antibiotics by HPLC using macrolide-imprinted synthetic polymers as stationary phases
M. Siemann, L. Andersson, and K. Mosbach. JOURNAL OF ANTIBIOTICS, 50 (1): 89-91 (January 1997)
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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1The structure of L-hydantoinase from Arthobacter aurescens leads to an understanding of dihydropyrimidinase substrate and enantio specificity
J. Abendroth, K. Niefind, O. May, M. Siemann, C. Syldatk, and D. Schomburg. BIOCHEMISTRY, 41 (27): 8589-8597 (July 2002)
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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3Escherichia coli HGT: Engineered for high glucose throughput even under slowly growing or resting conditions
A. Michalowski, M. Siemann-Herzberg, and R. Takors. METABOLIC ENGINEERING, (March 2017)
7 years ago by @siemannherzberg
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Escherichia
Nitrogen-limitation;
Stringent
coli}
glucose
myown
ppGpp;
response;
uptake;
{High
EscherichiaNitrogen-limitation;Stringentcoli}glucosemyownppGpp;response;uptake;{High
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1Process development of periplasmatically produced single chain fragment variable against epidermal growth factor receptor in Escherichia coli
R. Lindner, A. Moosmann, A. Dietrich, H. Boettinger, R. Kontermann, and M. Siemann-Herzberg. JOURNAL OF BIOTECHNOLOGY, 192 (A): 136-145 (December 2014)
7 years ago by @siemannherzberg
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Ion
Mixed
Periplasmic
chain
chromatography;
disruption}
exchange
fragment
mode
myown
variable;
{Single
IonMixedPeriplasmicchainchromatography;disruption}exchangefragmentmodemyownvariable;{Single
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1Topology of the global regulatory network of carbon limitation in Escherichia coli
T. Hardiman, K. Lemuth, M. Keller, M. Reuss, and M. Siemann-Herzberg. JOURNAL OF BIOTECHNOLOGY, 132 (4): 359-374 (December 2007)
7 years ago by @siemannherzberg
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analysis}
carbon
central
crp
cultivation;
fed-batch
flux
metabolic
metabolism;
modulon;
myown
response;
stringent
{cra
analysis}carboncentralcrpcultivation;fed-batchfluxmetabolicmetabolism;modulon;myownresponse;stringent{cra
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1Molecular evolution of hydantoinases
O. May, A. Habenicht, R. Mattes, C. Syldatk, and M. Siemann. BIOLOGICAL CHEMISTRY, 379 (6): 743-747 (June 1998)
7 years ago by @siemannherzberg
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acid
amidohydrolase;
amino
bioconversion;
enantioselectivity;
hydantoin
myown
production;
superfamily}
{amidase;
acidamidohydrolase;aminobioconversion;enantioselectivity;hydantoinmyownproduction;superfamily}{amidase;
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1Selective recognition of the herbicide atrazine by noncovalent molecularly imprinted polymers
M. Siemann, L. Andersson, and K. Mosbach. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 44 (1): 141-145 (January 1996)
7 years ago by @siemannherzberg
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antibody
assays;
binding
chromatography;
immunoassay
mimics;
myown
site
style
techniques}
{ligand
antibodyassays;bindingchromatography;immunoassaymimics;myownsitestyletechniques}{ligand
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1The Functional Structure of Central Carbon Metabolism in Pseudomonas putida KT2440
S. Sudarsan, S. Dethlefsen, L. Blank, M. Siemann-Herzberg, and A. Schmid. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 80 (17): 5292-5303 (September 2014)
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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1A D-specific hydantoin amidohydrolase: properties of the metalloenzyme purified from Arthrobacter crystallopoietes
M. Siemann, A. Alvarado-Marin, M. Pietzsch, and C. Syldatk. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 6 (3): 387-397 (March 1999)
7 years ago by @siemannherzberg
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acid}
amidase;
amino
cyclic
dihydropyrimidinase;
hydantoin;
myown
protein
purification;
urease;
{hydantoinase;
acid}amidase;aminocyclicdihydropyrimidinase;hydantoin;myownproteinpurification;urease;{hydantoinase;
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2Production of 1-Octanol from n-Octane by Pseudomonas putida KT2440
T. Vallon, M. Glemser, S. Malca, D. Scheps, J. Schmid, M. Siemann-Herzberg, B. Hauer, and R. Takors. CHEMIE INGENIEUR TECHNIK, 85 (6): 841-848 (June 2013)
7 years ago by @siemannherzberg
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Hydroxylation;
Two-phase
biotransformation}
myown
{Alkane;
Hydroxylation;Two-phasebiotransformation}myown{Alkane;
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1Substrate-dependent enantioselectivity of a novel hydantoinase from Arthrobacter aurescens DSM 3745: Purification and characterization as new member of cyclic amidases
O. May, M. Siemann, M. Pietzsch, M. Kiess, R. Mattes, and C. Syldatk. JOURNAL OF BIOTECHNOLOGY, 61 (1): 1-13 (March 1998)
7 years ago by @siemannherzberg
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EC
amidase;
aurescens;
hydantoinase;
myown
nomenclature;
physiology}
purification;
{Arthrobacter
ECamidase;aurescens;hydantoinase;myownnomenclature;physiology}purification;{Arthrobacter
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1High-cell-density fermentation for production of L-N-carbamoylase using an expression system based on the Escherichia coli rhaBAD promoter
B. Wilms, A. Hauck, M. Reuss, C. Syldatk, R. Mattes, M. Siemann, and J. Altenbuchner. BIOTECHNOLOGY AND BIOENGINEERING, 73 (2): 95-103 (April 2001)
7 years ago by @siemannherzberg
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L-N-carbamoylase;
expression;
fermentation;
high-cell-density
inducible
myown
plasmid
promoter}
rhamnose
stability;
{L-rhamnose
L-N-carbamoylase;expression;fermentation;high-cell-densityinduciblemyownplasmidpromoter}rhamnosestability;{L-rhamnose
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1CHARACTERIZATION OF SEROLOGICAL PROPERTIES OF POLYCLONAL ANTIBODIES PRODUCED AGAINST ENZYMES INVOLVED IN THE L-SELECTIVE CLEAVAGE OF HYDANTOIN DERIVATIVES
M. SIEMANN, C. SYLDATK, and F. WAGNER. BIOTECHNOLOGY LETTERS, 15 (1): 1-6 (January 1993)
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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1The hydantoin amidohydrolase from Arthrobacter aurescens DSM 3745 is a zinc metalloenzyme
O. May, M. Siemann, M. Siemann, and C. Syldatk. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 5 (1-4, SI): 367-370 (September 1998)3rd International Symposium on Biocatalysis and Biotransformations (BIOTRANS 97), LA GRANDE MOTTE, FRANCE, SEP 22-26, 1997.
7 years ago by @siemannherzberg
show all tags
Arthrobacter
amidase;
aurescens;
metalloenzyme}
myown
zinc
{hydantoinase;
Arthrobacteramidase;aurescens;metalloenzyme}myownzinc{hydantoinase;
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1Self-Inducible Bacillus subtilis Expression System for Reliable and Inexpensive Protein Production by High-Cell-Density Fermentation
M. Wenzel, A. Mueller, M. Siemann-Herzberg, and J. Altenbuchner. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77 (18): 6419-6425 (September 2011)
7 years ago by @siemannherzberg
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imported
myown
importedmyown
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1Catalytic and structural function of zinc for the hydantoinase from Arthrobacter aurescens DSM 3745
O. May, M. Siemann, M. Siemann, and C. Syldatk. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 4 (4): 211-218 (June 1998)
7 years ago by @siemannherzberg
show all tags
Arthrobacter
amidase;
aurescens;
cyclic
metalloenzyme;
myown
zinc}
{hydantoinase;
Arthrobacteramidase;aurescens;cyclicmetalloenzyme;myownzinc}{hydantoinase;
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