%0 Journal Article %1 ISI:000319434600007 %A Vallon, Tobias %A Glemser, Matthias %A Malca, Sumire Honda %A Scheps, Daniel %A Schmid, Joachim %A Siemann-Herzberg, Martin %A Hauer, Bernhard %A Takors, Ralf %C BOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANY %D 2013 %I WILEY-V C H VERLAG GMBH %J CHEMIE INGENIEUR TECHNIK %K Hydroxylation; Two-phase biotransformation} myown {Alkane; %N 6 %P 841-848 %R 10.1002/cite.201200178 %T Production of 1-Octanol from n-Octane by Pseudomonas putida KT2440 %U https://doi.org/10.1002/cite.201200178 %V 85 %X A two-phase biotransformation process for selective hydroxylation of n-octane to 1-octanol via Pseudomonas putida KT2440 harboring heterologously expressed P450 monooxygenase from Mycobacterium marinum is presented. Maximum cell-specific conversion rates of 12.7mg(octanol)g(CDW)h(-1) were observed not only in shaking flasks but also in 3.7-L-bioreactor studies. The bioreactor experiments were performed avoiding explosive gas mixtures by lowering volumetric power input, aeration rates and substrate concentrations. Based on a stoichiometric network of P. putida KT2440 topological studies were carried out. As a conclusion, potential limitations of NAD(P)H and/or ATP supply at production conditions can be excluded. Hence, the great potential of the host for further increasing conversion is outlined.

@article{ISI:000319434600007, abstract = {{A two-phase biotransformation process for selective hydroxylation of n-octane to 1-octanol via Pseudomonas putida KT2440 harboring heterologously expressed P450 monooxygenase from Mycobacterium marinum is presented. Maximum cell-specific conversion rates of 12.7mg(octanol)g(CDW)h(-1) were observed not only in shaking flasks but also in 3.7-L-bioreactor studies. The bioreactor experiments were performed avoiding explosive gas mixtures by lowering volumetric power input, aeration rates and substrate concentrations. Based on a stoichiometric network of P. putida KT2440 topological studies were carried out. As a conclusion, potential limitations of NAD(P)H and/or ATP supply at production conditions can be excluded. Hence, the great potential of the host for further increasing conversion is outlined.}}, added-at = {2018-01-25T13:38:08.000+0100}, address = {{BOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANY}}, affiliation = {{Vallon, T (Reprint Author), Univ Stuttgart, Inst Biochem Engn, Allmandring 31, D-70569 Stuttgart, Germany. Vallon, Tobias; Glemser, Matthias; Siemann-Herzberg, Martin; Takors, Ralf, Univ Stuttgart, Inst Biochem Engn, D-70569 Stuttgart, Germany. Malca, Sumire Honda; Scheps, Daniel; Hauer, Bernhard, Univ Stuttgart, Inst Tech Biochem, D-70569 Stuttgart, Germany. Schmid, Joachim, Insilico Biotechnol AG, D-70563 Stuttgart, Germany.}}, author = {Vallon, Tobias and Glemser, Matthias and Malca, Sumire Honda and Scheps, Daniel and Schmid, Joachim and Siemann-Herzberg, Martin and Hauer, Bernhard and Takors, Ralf}, author-email = {{takors@ibvt.uni-stuttgart.de}}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/2ee308aa4b4d43c42ad9845f723ac516f/siemannherzberg}, da = {{2018-01-25}}, doc-delivery-number = {{151BC}}, doi = {{10.1002/cite.201200178}}, funding-acknowledgement = {{German ministry of education and research; company BASF SE}}, funding-text = {{This study was supported by the German ministry of education and research and the company BASF SE. We thank Dr. Michael Breuer (BASF SE, Ludwigshafen, Germany) for supervising and coordinating the project consortium.}}, interhash = {79b6641fe97d8547e0bdf5dff073f76f}, intrahash = {ee308aa4b4d43c42ad9845f723ac516f}, issn = {{0009-286X}}, journal = {{CHEMIE INGENIEUR TECHNIK}}, journal-iso = {{Chem. Ing. Tech.}}, keywords = {Hydroxylation; Two-phase biotransformation} myown {Alkane;}, keywords-plus = {{ESCHERICHIA-COLI; ORGANIC-SOLVENTS; FUNCTIONAL EXPRESSION; ALKANE HYDROXYLASES; 2-LIQUID PHASE; CYTOCHROME-P450; CYP153A6; OXIDATION; ALCOHOLS; BACTERIA}}, language = {{English}}, month = {{JUN}}, number = {{6}}, number-of-cited-references = {{31}}, pages = {{841-848}}, publisher = {{WILEY-V C H VERLAG GMBH}}, research-areas = {{Engineering}}, times-cited = {{7}}, timestamp = {2018-01-25T12:38:18.000+0100}, title = {{Production of 1-Octanol from n-Octane by Pseudomonas putida KT2440}}, type = {{Article}}, unique-id = {{ISI:000319434600007}}, url = {https://doi.org/10.1002/cite.201200178}, usage-count-last-180-days = {{3}}, usage-count-since-2013 = {{24}}, volume = {{85}}, web-of-science-categories = {{Engineering, Chemical}}, year = {{2013}} }