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Towards the design of rare cutting restriction endonucleases: using directed evolution to generate variants of EcoRV differing in their substrate specificity by two orders of magnitude

T. Lanio, A. Jeltsch, und A. Pingoud. Journal of Molecular Biology, 283 (1): 59-69 (1998)
DOI: 10.1006/jmbi.1998.2088

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Dipl.-Ing. Thomas Kuestner

Scan time reduction in DWI of the pancreas using simultaneous multislice technique with different acceleration factors: how fast can we go?J. Taron, P. Martirosian, T. Küstner, M. Notohamiprodjo, J. Weiß, A. Othman, K. Nikolaou, und C. Schraml. Proceedings of the International Society for Magnetic Resonance in Medicine (ISMRM), Honolulu, Hawaii, USA, (April 2017)

Scan time reduction in DWI of the pancreas using simultaneous multislice technique with different acceleration factors: how fast can we go?J. Taron, P. Martirosian, T. Küstner, M. Notohamiprodjo, J. Weiß, A. Othman, K. Nikolaou, und C. Schraml. Proceedings of the International Society for Magnetic Resonance in Medicine (ISMRM), Honolulu, Hawaii, USA, (April 2017)The use of a generalized reconstruction by inversion of coupled systems (GRICS) approach for generic respiratory motion correction in PET/MR imagingH. Fayad, F. Odille, H. Schmidt, C. Würslin, T. Küstner, J. Felblinger, und D. Visvikis. Physics in medicine and biology, 60 (6): 2529-46 (März 2015)In-vivo MR-derived non-rigid motion correction of simultaneously acquired PETT. Küstner, C. Würslin, S. Gatidis, P. Martirosian, N. Schwenzer, B. Yang, und H. Schmidt. Proceedings of the International Society for Magnetic Resonance in Medicine (ISMRM), Toronto, Canada, (Juni 2015)

Univ. -Prof. Dr. Thomas Graf University of Stuttgart

Bending of Lloyd’s mirror to eliminate the period chirp in the fabrication of diffraction gratingsF. Bienert, C. Röcker, T. Graf, und M. Ahmed. Optics Express, 32 (10): 18430 (Mai 2024)

Thomas Maier

Thomas Maier

Thomas Maier

Weitere Publikationen von Autoren mit dem selben Namen

Developing a programmed restriction endonuclease for highly specific DNA cleavageK. Eisenschmidt, T. Lanio, A. Simoncsits, A. Jeltsch, V. Pingoud, W. Wende, und A. Pingoud. Nucleic Acids Research, 33 (22): 7039-7047 (2005)Structural and biochemical characterization of a new Mg(2+) binding site near Tyr94 in the restriction endonuclease PvuIIA. Spyridaki, C. Matzen, T. Lanio, A. Jeltsch, A. Simoncsits, A. Athanasiadis, E. Scheuring-Vanamee, M. Kokkinidis, und A. Pingoud. Journal of Molecular Biology, 331 (2): 395-406 (2003)Protein engineering of the restriction endonuclease EcoRV--structure-guided design of enzyme variants that recognize the base pairs flanking the recognition siteS. Schöttler, C. Wenz, T. Lanio, A. Jeltsch, und A. Pingoud. European Journal of Biochemistry, 258 (1): 184-191 (1998)Towards the design of rare cutting restriction endonucleases: using directed evolution to generate variants of EcoRV differing in their substrate specificity by two orders of magnitudeT. Lanio, A. Jeltsch, und A. Pingoud. Journal of Molecular Biology, 283 (1): 59-69 (1998)High-throughput purification of polyHis-tagged recombinant fusion proteinsT. Lanio, A. Jeltsch, und A. Pingoud. E. coli Gene Expression Protocols, 205, Kapitel 12, Humana Press, Totowa, NJ, (2003)Automated purification of His6-tagged proteins allows exhaustive screening of libraries generated by random mutagenesisT. Lanio, A. Jeltsch, und A. Pingoud. BioTechniques, 29 (2): 338-342 (2000)A fluorimetric assay for on-line detection of DNA cleavage by restriction endonucleasesK. Eisenschmidt, T. Lanio, A. Jeltsch, und A. Pingoud. Journal of Biotechnology, 96 (2): 185-191 (2002)Site-directed mutagenesis by polymerase chain reactionA. Jeltsch, und T. Lanio. In Vitro Mutagenesis Protocols, 182, Kapitel 11, Humana Press, Totowa, NJ, 2 Edition, (2002)On the possibilities and limitations of rational protein design to expand the specificity of restriction enzymes: a case study employing EcoRV as the targetT. Lanio, A. Jeltsch, und A. Pingoud. Protein engineering, 13 (4): 275-281 (2000)PCR-based random mutagenesis method using spiked oligonucleotides to randomize selected parts of a gene without any wild-type backgroundT. Lanio, und A. Jeltsch. BioTechniques, 25 (6): 958-965 (1998)