%0 Generic %1 hagele2024getting %A Hägele, Lorena %A Takors, Ralf %D 2024 %K darus ubs_10004 ubs_20005 ubs_30053 ubs_40077 unibibliografie %R 10.18419/darus-4355 %T Getting the right clones in an automated manner: an alternative to sophisticated colony-picking robotics %X In recent years, the Design-Build-Test-Learn (DBTL) cycle has become a key concept in strain engineering. Modern biofoundries enable automated DBTL cycling using robotic devices. However, both highly automated facilities and semi-automated facilities encounter bottlenecks in clone selection and screening. While fully automated biofoundries can take advantage of expensive commercially available colony picker, semi-automated facilities have to fall back on affordable alternatives. Therefore, our clone selection method is particularly well-suited for academic settings, requiring only basic infrastructure of a biofoundry.The automated liquid clone selection method (ALCS) represents a straightforward approach for clone selection. Similar to sophisticated colony picking robots, the ALCS approach aims to achieve high selectivity. Investigating the time analogue of five generations, the model-based set-up reached a selectivity of 98 ± 0.2 % for correctly transformed cells. Moreover, the method is robust to variations in cell numbers at the start of ALCS. Beside Escherichia coli, promising chassis organisms, such as Pseudomonas putida and Corynebacterium glutamicum, were successfully applied. In all cases, ALCS enables the immediate use of the selected strains in follow-up applications. In essence, our ALCS approach provides a ‘low-tech’ method to be implemented in biofoundry settings without requiring additional devices.

@misc{hagele2024getting, abstract = {In recent years, the Design-Build-Test-Learn (DBTL) cycle has become a key concept in strain engineering. Modern biofoundries enable automated DBTL cycling using robotic devices. However, both highly automated facilities and semi-automated facilities encounter bottlenecks in clone selection and screening. While fully automated biofoundries can take advantage of expensive commercially available colony picker, semi-automated facilities have to fall back on affordable alternatives. Therefore, our clone selection method is particularly well-suited for academic settings, requiring only basic infrastructure of a biofoundry.The automated liquid clone selection method (ALCS) represents a straightforward approach for clone selection. Similar to sophisticated colony picking robots, the ALCS approach aims to achieve high selectivity. Investigating the time analogue of five generations, the model-based set-up reached a selectivity of 98 ± 0.2 % for correctly transformed cells. Moreover, the method is robust to variations in cell numbers at the start of ALCS. Beside Escherichia coli, promising chassis organisms, such as Pseudomonas putida and Corynebacterium glutamicum, were successfully applied. In all cases, ALCS enables the immediate use of the selected strains in follow-up applications. In essence, our ALCS approach provides a ‘low-tech’ method to be implemented in biofoundry settings without requiring additional devices. }, added-at = {2024-08-05T15:57:38.000+0200}, affiliation = {Hägele, Lorena/University of Stuttgart, Takors, Ralf/University of Stuttgart}, author = {Hägele, Lorena and Takors, Ralf}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/2bef0475d7ff8976cbfde4bbff73fc7f6/unibiblio}, doi = {10.18419/darus-4355}, howpublished = {Dataset}, interhash = {3a37f48c5b111ec9e8328453613d45ff}, intrahash = {bef0475d7ff8976cbfde4bbff73fc7f6}, keywords = {darus ubs_10004 ubs_20005 ubs_30053 ubs_40077 unibibliografie}, orcid-numbers = {Hägele, Lorena/0009-0001-6624-3457, Takors, Ralf/0000-0001-5837-6906}, timestamp = {2025-03-11T09:41:10.000+0100}, title = {Getting the right clones in an automated manner: an alternative to sophisticated colony-picking robotics}, year = 2024 }