%0 Journal Article %1 CM:CM21003 %A Viguès, Bernard %A Damaj, Raghida %D 2012 %I John Wiley & Sons, Inc. %J Cytoskeleton %K adhesive ciliate disc trichodina %N 2 %P 113--124 %R 10.1002/cm.21003 %T The adhesive disc in the mobilid ciliate Trichodina pediculus: Evidence for centrin-related, calcium-sensitive filaments %U http://dx.doi.org/10.1002/cm.21003 %V 69 %X The adhesive disc is a highly complex apparatus that allows mobilid ciliates to attach to the tissues of a variety of aquatic invertebrates and vertebrates. The disc comprises concentric rings of rigid skeletal pieces interconnected by filamentous material. This study explored the biochemical properties of the filamentous disc material in the trichodinid Trichodina pediculus. Calcium sensitivity of this material was suggested in vitro by the appearance of transverse cross-striation along bundles of filaments following calcium shock, and complete solubilization of the filamentous material in the presence of EGTA. A 23-kDa immunoanalog of centrins was immunoprecipitated from the EGTA extract. The protein binds calcium as indicated by 45Ca2+ blot overlay and Ca2+-induced shifts in electrophoretic mobility. Using Ca2+/EGTA buffers, we demonstrated a direct relationship between extraction of the filaments and solubilization of the protein. Immunofluorescence and immunoelectron microscopy confirmed that the protein localized to the filamentous disc material and revealed cross-reactivity with the spasmoneme, which is the prototype of ion-sensitive, centrin-like contractile systems in ciliates. The possibility that the filamentous disc material may be a novel example of Ca2+-sensitive, centrin-based systems found in ciliates is discussed. © 2011 Wiley Periodicals Inc.

@article{CM:CM21003, abstract = {The adhesive disc is a highly complex apparatus that allows mobilid ciliates to attach to the tissues of a variety of aquatic invertebrates and vertebrates. The disc comprises concentric rings of rigid skeletal pieces interconnected by filamentous material. This study explored the biochemical properties of the filamentous disc material in the trichodinid Trichodina pediculus. Calcium sensitivity of this material was suggested in vitro by the appearance of transverse cross-striation along bundles of filaments following calcium shock, and complete solubilization of the filamentous material in the presence of EGTA. A 23-kDa immunoanalog of centrins was immunoprecipitated from the EGTA extract. The protein binds calcium as indicated by 45Ca2+ blot overlay and Ca2+-induced shifts in electrophoretic mobility. Using Ca2+/EGTA buffers, we demonstrated a direct relationship between extraction of the filaments and solubilization of the protein. Immunofluorescence and immunoelectron microscopy confirmed that the protein localized to the filamentous disc material and revealed cross-reactivity with the spasmoneme, which is the prototype of ion-sensitive, centrin-like contractile systems in ciliates. The possibility that the filamentous disc material may be a novel example of Ca2+-sensitive, centrin-based systems found in ciliates is discussed. © 2011 Wiley Periodicals Inc.}, added-at = {2017-05-18T15:05:22.000+0200}, author = {Viguès, Bernard and Damaj, Raghida}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/241390cc05db393dca179542e4d57051a/clara}, doi = {10.1002/cm.21003}, interhash = {9138578c00a5e74edea7a73ec5fc9ed3}, intrahash = {41390cc05db393dca179542e4d57051a}, issn = {1949-3592}, journal = {Cytoskeleton}, keywords = {adhesive ciliate disc trichodina}, number = 2, pages = {113--124}, publisher = {John Wiley & Sons, Inc.}, timestamp = {2017-05-18T13:05:22.000+0200}, title = {The adhesive disc in the mobilid ciliate Trichodina pediculus: Evidence for centrin-related, calcium-sensitive filaments}, url = {http://dx.doi.org/10.1002/cm.21003}, volume = 69, year = 2012 }