%0 Journal Article %1 ISI:A1993KL06500001 %A SIEMANN, M %A SYLDATK, C %A WAGNER, F %C 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8HN %D 1993 %I CHAPMAN HALL LTD %J BIOTECHNOLOGY LETTERS %K imported myown %N 1 %P 1-6 %R 10.1007/BF00131543 %T CHARACTERIZATION OF SEROLOGICAL PROPERTIES OF POLYCLONAL ANTIBODIES PRODUCED AGAINST ENZYMES INVOLVED IN THE L-SELECTIVE CLEAVAGE OF HYDANTOIN DERIVATIVES %U https://doi.org/10.1007/BF00131543 %V 15 %X Polyclonal antibodies were produced against the highly purified enzymes L-hydantoinase, hydantoin-racemase and L-N-carbamoylamino acid amidohydrolase of Arthrobacter aurescens DSM 3747. In order to exploit these antibodies for basic research (molecular biology) or bioengineering (process development), the serological properties had to be characterized. Both, the hydantoinase- and carbamoylase-antibodies were observed to be monofunctional, whereas the hydantoin-racemase-antibody was found to be additionally specific against the L-hydantoinase. Monospecificity was realized after affinity chromatography. Investigations on serological crossreactions with several linear- and cyclic amidases (e.g. hydantoinases) as well as hydantoin-racemases are demonstrated in this paper.

@article{ISI:A1993KL06500001, abstract = {{Polyclonal antibodies were produced against the highly purified enzymes L-hydantoinase, hydantoin-racemase and L-N-carbamoylamino acid amidohydrolase of Arthrobacter aurescens DSM 3747. In order to exploit these antibodies for basic research (molecular biology) or bioengineering (process development), the serological properties had to be characterized. Both, the hydantoinase- and carbamoylase-antibodies were observed to be monofunctional, whereas the hydantoin-racemase-antibody was found to be additionally specific against the L-hydantoinase. Monospecificity was realized after affinity chromatography. Investigations on serological crossreactions with several linear- and cyclic amidases (e.g. hydantoinases) as well as hydantoin-racemases are demonstrated in this paper.}}, added-at = {2018-01-25T13:38:08.000+0100}, address = {{2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8HN}}, affiliation = {{TECH UNIV BRAUNSCHWEIG, INST BIOCHEM \& BIOTECHNOL, KONSTANTIN UHDE STR 5, W-3300 BRAUNSCHWEIG, GERMANY.}}, author = {SIEMANN, M and SYLDATK, C and WAGNER, F}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/2e50c49afd37eea16ec8f5955043836f2/siemannherzberg}, da = {{2018-01-25}}, doc-delivery-number = {{KL065}}, doi = {{10.1007/BF00131543}}, interhash = {a67c51ac1493b3a8e8b3c3e6b54c2814}, intrahash = {e50c49afd37eea16ec8f5955043836f2}, issn = {{0141-5492}}, journal = {{BIOTECHNOLOGY LETTERS}}, journal-iso = {{Biotechnol. Lett.}}, keywords = {imported myown}, language = {{English}}, month = {{JAN}}, number = {{1}}, number-of-cited-references = {{9}}, pages = {{1-6}}, publisher = {{CHAPMAN HALL LTD}}, research-areas = {{Biotechnology \& Applied Microbiology}}, times-cited = {{6}}, timestamp = {2018-01-25T12:38:18.000+0100}, title = {{CHARACTERIZATION OF SEROLOGICAL PROPERTIES OF POLYCLONAL ANTIBODIES PRODUCED AGAINST ENZYMES INVOLVED IN THE L-SELECTIVE CLEAVAGE OF HYDANTOIN DERIVATIVES}}, type = {{Article}}, unique-id = {{ISI:A1993KL06500001}}, url = {https://doi.org/10.1007/BF00131543}, usage-count-last-180-days = {{0}}, usage-count-since-2013 = {{1}}, volume = {{15}}, web-of-science-categories = {{Biotechnology \& Applied Microbiology}}, year = {{1993}} }