%0 Journal Article %1 blombach_effect_2007 %A Blombach, Bastian %A Schreiner, Mark E. %A Moch, Matthias %A Oldiges, Marco %A Eikmanns, Bernhard J. %D 2007 %J Appl. Microbiol. Biotechnol. %K Bacterial, Base Biotechnology Complex, Corynebacterium DNA, Dehydrogenase Deletion, Expression, Fermentation, Gene Genes, Kinetics, Lysine, Pyruvate Sequence, glutamicum, myown %N 3 %P 615--623 %R 10.1007/s00253-007-0904-1 %T Effect of pyruvate dehydrogenase complex deficiency on L-lysine production with Corynebacterium glutamicum %V 76 %X Intracellular precursor supply is a critical factor for amino acid productivity of Corynebacterium glutamicum. To test for the effect of improved pyruvate availability on L-lysine production, we deleted the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex (PDHC) in the L-lysine-producer C. glutamicum DM1729 and characterised the resulting strain DM1729-BB1 for growth and L-lysine production. Compared to the host strain, C. glutamicum DM1729-BB1 showed no PDHC activity, was acetate auxotrophic and, after complete consumption of the available carbon sources glucose and acetate, showed a more than 50\% lower substrate-specific biomass yield (0.14 vs 0.33 mol C/mol C), an about fourfold higher biomass-specific L-lysine yield (5.27 vs 1.23 mmol/g cell dry weight) and a more than 40\% higher substrate-specific L-lysine yield (0.13 vs 0.09 mol C/mol C). Overexpression of the pyruvate carboxylase or diaminopimelate dehydrogenase genes in C. glutamicum DM1729-BB1 resulted in a further increase in the biomass-specific L-lysine yield by 6 and 56\%, respectively. In addition to L-lysine, significant amounts of pyruvate, L-alanine and L-valine were produced by C. glutamicum DM1729-BB1 and its derivatives, suggesting a surplus of precursor availability and a further potential to improve L-lysine production by engineering the L-lysine biosynthetic pathway.

@article{blombach_effect_2007, abstract = {Intracellular precursor supply is a critical factor for amino acid productivity of Corynebacterium glutamicum. To test for the effect of improved pyruvate availability on L-lysine production, we deleted the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex (PDHC) in the L-lysine-producer C. glutamicum DM1729 and characterised the resulting strain DM1729-BB1 for growth and L-lysine production. Compared to the host strain, C. glutamicum DM1729-BB1 showed no PDHC activity, was acetate auxotrophic and, after complete consumption of the available carbon sources glucose and acetate, showed a more than 50\% lower substrate-specific biomass yield (0.14 vs 0.33 mol C/mol C), an about fourfold higher biomass-specific L-lysine yield (5.27 vs 1.23 mmol/g cell dry weight) and a more than 40\% higher substrate-specific L-lysine yield (0.13 vs 0.09 mol C/mol C). Overexpression of the pyruvate carboxylase or diaminopimelate dehydrogenase genes in C. glutamicum DM1729-BB1 resulted in a further increase in the biomass-specific L-lysine yield by 6 and 56\%, respectively. In addition to L-lysine, significant amounts of pyruvate, L-alanine and L-valine were produced by C. glutamicum DM1729-BB1 and its derivatives, suggesting a surplus of precursor availability and a further potential to improve L-lysine production by engineering the L-lysine biosynthetic pathway.}, added-at = {2018-02-09T13:18:17.000+0100}, author = {Blombach, Bastian and Schreiner, Mark E. and Moch, Matthias and Oldiges, Marco and Eikmanns, Bernhard J.}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/2930ecfe113b73f241ab0452b05337d89/bastian}, doi = {10.1007/s00253-007-0904-1}, interhash = {97097cd65b8e231a2578efbc7586a5dc}, intrahash = {930ecfe113b73f241ab0452b05337d89}, issn = {0175-7598}, journal = {Appl. Microbiol. Biotechnol.}, keywords = {Bacterial, Base Biotechnology Complex, Corynebacterium DNA, Dehydrogenase Deletion, Expression, Fermentation, Gene Genes, Kinetics, Lysine, Pyruvate Sequence, glutamicum, myown}, language = {eng}, month = sep, number = 3, pages = {615--623}, pmid = {17333167}, timestamp = {2018-02-09T12:18:56.000+0100}, title = {Effect of pyruvate dehydrogenase complex deficiency on {L}-lysine production with {Corynebacterium} glutamicum}, volume = 76, year = 2007 }