Zusammenfassung
A two-phase biotransformation process for selective hydroxylation of
n-octane to 1-octanol via Pseudomonas putida KT2440 harboring
heterologously expressed P450 monooxygenase from Mycobacterium marinum
is presented. Maximum cell-specific conversion rates of
12.7mg(octanol)g(CDW)h(-1) were observed not only in shaking flasks but
also in 3.7-L-bioreactor studies. The bioreactor experiments were
performed avoiding explosive gas mixtures by lowering volumetric power
input, aeration rates and substrate concentrations. Based on a
stoichiometric network of P. putida KT2440 topological studies were
carried out. As a conclusion, potential limitations of NAD(P)H and/or
ATP supply at production conditions can be excluded. Hence, the great
potential of the host for further increasing conversion is outlined.
Nutzer
