%0 Journal Article %1 lange_valorization_2017 %A Lange, Julian %A Müller, Felix %A Bernecker, Kerstin %A Dahmen, Nicolaus %A Takors, Ralf %A Blombach, Bastian %D 2017 %J Biotechnol Biofuels %K (propylene 1,2-propanediol Bioeconomy, Biorefinery, Corynebacterium Fast Growth-coupled Lignocellulose, Metabolic Pyrolysis biotransformation, engineering, glutamicum, glycol), myown pyrolysis, water %P 277 %R 10.1186/s13068-017-0969-8 %T Valorization of pyrolysis water: a biorefinery side stream, for 1,2-propanediol production with engineered Corynebacterium glutamicum %V 10 %X Background: A future bioeconomy relies on the efficient use of renewable resources for energy and material product supply. In this context, biorefineries have been developed and play a key role in converting lignocellulosic residues. Although a holistic use of the biomass feed is desired, side streams evoke in current biorefinery approaches. To ensure profitability, efficiency, and sustainability of the overall conversion process, a meaningful valorization of these materials is needed. Here, a so far unexploited side stream derived from fast pyrolysis of wheat straw-pyrolysis water-was used for production of 1,2-propanediol in microbial fermentation with engineered Corynebacterium glutamicum. Results: A protocol for pretreatment of pyrolysis water was established and enabled growth on its major constituents, acetate and acetol, with rates up to 0.36 ± 0.04 h-1. To convert acetol to 1,2-propanediol, the plasmid pJULgldA expressing the glycerol dehydrogenase from Escherichia coli was introduced into C. glutamicum. 1,2-propanediol was formed in a growth-coupled biotransformation and production was further increased by construction of C. glutamicum Δpqo ΔaceE ΔldhA Δmdh pJULgldA. In a two-phase aerobic/microaerobic fed-batch process with pyrolysis water as substrate, this strain produced 18.3 ± 1.2 mM 1,2-propanediol with a yield of 0.96 ± 0.05 mol 1,2-propanediol per mol acetol and showed an overall volumetric productivity of 1.4 ± 0.1 mmol 1,2-propanediol L-1 h-1. Conclusions: This study implements microbial fermentation into a biorefinery based on pyrolytic liquefaction of lignocellulosic biomass and accesses a novel value chain by valorizing the side stream pyrolysis water for 1,2-PDO production with engineered C. glutamicum. The established bioprocess operated at maximal product yield and accomplished the so far highest overall volumetric productivity for microbial 1,2-PDO production with an engineered producer strain. Besides, the results highlight the potential of microbial conversion of this biorefinery side stream to other valuable products.

@article{lange_valorization_2017, abstract = {Background: A future bioeconomy relies on the efficient use of renewable resources for energy and material product supply. In this context, biorefineries have been developed and play a key role in converting lignocellulosic residues. Although a holistic use of the biomass feed is desired, side streams evoke in current biorefinery approaches. To ensure profitability, efficiency, and sustainability of the overall conversion process, a meaningful valorization of these materials is needed. Here, a so far unexploited side stream derived from fast pyrolysis of wheat straw-pyrolysis water-was used for production of 1,2-propanediol in microbial fermentation with engineered Corynebacterium glutamicum. Results: A protocol for pretreatment of pyrolysis water was established and enabled growth on its major constituents, acetate and acetol, with rates up to 0.36 ± 0.04 h-1. To convert acetol to 1,2-propanediol, the plasmid pJULgldA expressing the glycerol dehydrogenase from Escherichia coli was introduced into C. glutamicum. 1,2-propanediol was formed in a growth-coupled biotransformation and production was further increased by construction of C. glutamicum Δpqo ΔaceE ΔldhA Δmdh pJULgldA. In a two-phase aerobic/microaerobic fed-batch process with pyrolysis water as substrate, this strain produced 18.3 ± 1.2 mM 1,2-propanediol with a yield of 0.96 ± 0.05 mol 1,2-propanediol per mol acetol and showed an overall volumetric productivity of 1.4 ± 0.1 mmol 1,2-propanediol L-1 h-1. Conclusions: This study implements microbial fermentation into a biorefinery based on pyrolytic liquefaction of lignocellulosic biomass and accesses a novel value chain by valorizing the side stream pyrolysis water for 1,2-PDO production with engineered C. glutamicum. The established bioprocess operated at maximal product yield and accomplished the so far highest overall volumetric productivity for microbial 1,2-PDO production with an engineered producer strain. Besides, the results highlight the potential of microbial conversion of this biorefinery side stream to other valuable products.}, added-at = {2018-02-09T13:18:17.000+0100}, author = {Lange, Julian and Müller, Felix and Bernecker, Kerstin and Dahmen, Nicolaus and Takors, Ralf and Blombach, Bastian}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/27ae233f38758c480db193ed5e7bbf7f7/bastian}, doi = {10.1186/s13068-017-0969-8}, interhash = {dc474d4c00bd416fa2ec00ea366a50da}, intrahash = {7ae233f38758c480db193ed5e7bbf7f7}, issn = {1754-6834}, journal = {Biotechnol Biofuels}, keywords = {(propylene 1,2-propanediol Bioeconomy, Biorefinery, Corynebacterium Fast Growth-coupled Lignocellulose, Metabolic Pyrolysis biotransformation, engineering, glutamicum, glycol), myown pyrolysis, water}, language = {eng}, pages = 277, pmcid = {PMC5697356}, pmid = {29201141}, shorttitle = {Valorization of pyrolysis water}, timestamp = {2018-02-09T12:18:56.000+0100}, title = {Valorization of pyrolysis water: a biorefinery side stream, for 1,2-propanediol production with engineered {Corynebacterium} glutamicum}, volume = 10, year = 2017 }