Antibody fragments, especially single-chain Fv fragments, have been established for the generation of immunoliposomes for targeted drug delivery in cancer therapy and other applications. Bispecific immunoliposomes should be useful for dual targeting addressing inter- and intratumoral heterogeneity of tumor antigen expression. Here, we established a protocol to generate dual-targeted immunoliposomes using genetically engineered scFv molecules recognizing two different tumor-associated antigens, EGFR and CEA (CEACAM5), applying a step-wise insertion of antibody-coupled micelles into preformed PEGylated liposomes. The dual-targeted immunoliposomes retained binding activity for both antigens and combined the selectivity of both antibodies within one liposome. Thus, these dual-targeted immunoliposomes should be suitable to deliver therapeutic payloads to tumor cells expressing EGFR or CEA, or both antigens.
%0 Journal Article
%1 Mack2012
%A Mack, Katharina
%A Rüger, Ronny
%A Fellermeier, Sina
%A Seifert, Oliver
%A Kontermann, Roland E.
%D 2012
%I Molecular Diversity Preservation International
%J Antibodies
%K 2012 izi kontermann
%N 3
%P 199--214
%R 10.3390/antib1020199
%T Dual Targeting of Tumor Cells with Bispecific Single-Chain Fv-Immunoliposomes
%U http://www.mdpi.com/2073-4468/1/2/199/
%V 1
%X Antibody fragments, especially single-chain Fv fragments, have been established for the generation of immunoliposomes for targeted drug delivery in cancer therapy and other applications. Bispecific immunoliposomes should be useful for dual targeting addressing inter- and intratumoral heterogeneity of tumor antigen expression. Here, we established a protocol to generate dual-targeted immunoliposomes using genetically engineered scFv molecules recognizing two different tumor-associated antigens, EGFR and CEA (CEACAM5), applying a step-wise insertion of antibody-coupled micelles into preformed PEGylated liposomes. The dual-targeted immunoliposomes retained binding activity for both antigens and combined the selectivity of both antibodies within one liposome. Thus, these dual-targeted immunoliposomes should be suitable to deliver therapeutic payloads to tumor cells expressing EGFR or CEA, or both antigens.
%@ 4971168567
@article{Mack2012,
abstract = {Antibody fragments, especially single-chain Fv fragments, have been established for the generation of immunoliposomes for targeted drug delivery in cancer therapy and other applications. Bispecific immunoliposomes should be useful for dual targeting addressing inter- and intratumoral heterogeneity of tumor antigen expression. Here, we established a protocol to generate dual-targeted immunoliposomes using genetically engineered scFv molecules recognizing two different tumor-associated antigens, EGFR and CEA (CEACAM5), applying a step-wise insertion of antibody-coupled micelles into preformed PEGylated liposomes. The dual-targeted immunoliposomes retained binding activity for both antigens and combined the selectivity of both antibodies within one liposome. Thus, these dual-targeted immunoliposomes should be suitable to deliver therapeutic payloads to tumor cells expressing EGFR or CEA, or both antigens.},
added-at = {2018-02-01T16:20:43.000+0100},
author = {Mack, Katharina and R{\"{u}}ger, Ronny and Fellermeier, Sina and Seifert, Oliver and Kontermann, Roland E.},
biburl = {https://puma.ub.uni-stuttgart.de/bibtex/2017a887a61b5b6d2269b2e85ff8f0c40/cristiano},
doi = {10.3390/antib1020199},
interhash = {ff2fe6d842c1877344a8c3ca090bcb8c},
intrahash = {017a887a61b5b6d2269b2e85ff8f0c40},
isbn = {4971168567},
issn = {2073-4468},
journal = {Antibodies},
keywords = {2012 izi kontermann},
month = jul,
number = 3,
pages = {199--214},
publisher = {Molecular Diversity Preservation International},
timestamp = {2019-01-17T13:19:44.000+0100},
title = {{Dual Targeting of Tumor Cells with Bispecific Single-Chain Fv-Immunoliposomes}},
url = {http://www.mdpi.com/2073-4468/1/2/199/},
volume = 1,
year = 2012
}
