Systemic intracellular analysis for balancing complex biosynthesis in a transcriptionally deregulated Escherichia coli L-Methionine producer. Microbial Biotechnology, (17)e14433 2024. [PUMA: myown]
Mimicking CHO large‐scale effects in the single multicompartment bioreactor: A new approach to access scale‐up behavior. Biotechnology & Bioengineering, 2024. [PUMA: myown]
Experimental analysis of lifelines in a 15,000 L bioreactor by means of Lagrangian Sensor Particles. Chemical Engineering Research and Design, (205):695-712, 2024. [PUMA: myown]
Synthetic co‑culture in an interconnected two‑compartment bioreactor system: violacein production with recombinant E. coli strains. Bioprocess and Biosystems Engineering, 2024. [PUMA: myown]
Optimizing Mass Transfer in Multiphase Fermentation: The Role of Drag Models and Physical Conditions. processes, (12)452023. [PUMA: myown]
Novel experimental data-driven bubble breakage model for universal application in Euler-Lagrange multiphase frameworks. Chemical Engineering Science, (284)1195092023. [PUMA: myown]
Reactive extraction of pyridinedicarboxylic acid (PDCA) with tri-n-octylamine and Aliquat 336 in 1-octanol – A promising approach to access microbially produced PDCA. Separation and Purification Technology, (333)1258262023. [PUMA: myown]
Metabolic engineering of Corynebacterium glutamicum for fatty alcohol production from glucose and wheat straw hydrolysate. Biotechnology for Biofuels and Bioproducts, (16)1162023. [PUMA: myown]
Cell Cycle Control by Optogenetically Regulated Cell Cycle Inhibitor Protein p21. Biology, (12)92023. [PUMA: myown]
Growth-rate dependency of ribosome abundance and translation elongation rate in Corynebacterium glutamicum differs from that in Escherichia coli. Nature Communications, (14)56112023. [PUMA: myown]
The pH-dependent lactose metabolism of Lactobacillus delbrueckii subsp. bulgaricus: An integrative view through a mechanistic computational model. J Biotechnol, 374:90-100, 2023. [PUMA: myown]
Milligrams to kilograms: making microbes work at scale. TIBTEC, 2023. [PUMA: myown]
Mimicked Mixing-Induced Heterogeneities of Industrial Bioreactors Stimulate Long-Lasting Adaption Programs in Ethanol-Producing Yeasts. genes, (14)9972023. [PUMA: myown]
Recent progress in the synthesis of advanced biofuel and bioproducts. Current Opinion in Biotechnology, (80)102913:1-10, 2023. [PUMA: myown]
Transferring Bubble Breakage Models Tailored for Euler-Euler Approaches to Euler-Lagrange Simulations. processes, (11)1018:1-22, 2023. [PUMA: myown]
Revisiting Basics of kLa Dependency on Aeration in Bubble Columns: a Is Surprisingly Stable. Chemie Ingenieur Technik, (95)4:1-8, 2023. [PUMA: myown]
A Two‐Compartment Fermentation System to Quantify Strain‐Specific Interactions in Microbial Co‐Cultures. Bioengineering, (10)1032023. [PUMA: myown]
Performing in spite of starvation: How Saccharomyces cerevisiae maintains robust growth when facing famine zones in industrial bioreactors. Microbial Biotechnology, (16)1:148--168, Wiley, December 2022. [PUMA: myown] URL
Monitoring Intracellular Metabolite Dynamics in Saccharomyces cerevisiae during Industrially Relevant Famine Stimuli. metabolites, (12)2632022. [PUMA: myown]
FAIR research data management as community approach in bioengineering. Engineering in Life Science, 2022. [PUMA: myown]
Polyphosphate Kinases Phosphorylate Thiamine Phosphates. Microbial Physiology, 2022. [PUMA: myown]
Scaling-down biopharmaceutical production processes via a single multi-compartment bioreactor (SMCB). Engineering in life sciences, 2022. [PUMA: myown]
Investigation of tracer gas transport in a new numerical model of lung acini. Medical & Biological Engineering & Computing, 2022. [PUMA: myown]
Differential Amino Acid Uptake and Depletion in Mono-Cultures and Co-Cultures of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus in a Novel Semi-Synthetic Medium. microorganisms, (10)17712022. [PUMA: myown]
Synthetic mutualism in engineered E. coli mutant strains as functional basis for microbial production consortia. Engineering in Life Science, 1-14, 2022. [PUMA: myown]
CRISPRi enables fast growth followed by stable aerobic pyruvate formation in Escherichia coli without auxotrophy. Engineering in Life Science, 2021. [PUMA: myown]
Pseudomonas putida KT2440 endures temporary oxygen limitations. Biotechnology and Bioengineering, 2021. [PUMA: myown]
Towards valorization of pectin-rich agro-industrial residues: Engineering of Saccharomyces cerevisiae for co-fermentation of d-galacturonic acid and glycerol. Metabolic Engineering, 2021. [PUMA: myown]
Compartment‑specific metabolome labeling enables the identification of subcellular fluxes that may serve as promising metabolic engineering targets in CHO cells. Bioprocess and Biosystems Engineering, 2021. [PUMA: myown]
Compartment-specific 13C metabolic flux analysis reveals boosted NADPH availability coinciding with increased cell-specific productivity for IgG1 producing CHO cells after MTA treatment. Engineering in Life Science, 2021. [PUMA: myown]
Engineering a robust Escherichia coli chassis and exploitation for large-scale production process. Metabolic Engineering, (67):75-87, 2021. [PUMA: myown]
Isobutanol Production by Autotrophic Acetogenic Bacteria. Frontiers in Bioengineering and Biotechnology, 2021. [PUMA: myown]
A transhydrogenase-like mechanism in CHO cells comprising concerted cytosolic reaction and mitochondrial shuttling activities. Biochemical Engineering Journal, (170)2021. [PUMA: myown]
Identifying and engineering bottlenecks of autotrophic isobutanol formation in recombinant C. ljungdahlii by systemic analysis. Frontiers in Bioengineering and Biotechnology, (9)6478532021. [PUMA: myown]
Micro-aerobic production of isobutanol with engineered Pseudomonas putida. Engineering in Life Science, 2021. [PUMA: myown]
Comprehensive Analysis of C. glutamicum Anaplerotic Deletion Mutants Under Defined D-Glucose Conditions. Frontiers in Bioengineering and Biotechnology, (8)6039362021. [PUMA: myown]
Balancing glucose and oxygen uptake rates to enable high amorpha‐4,11‐diene production in Escherichia coli via the methylerythritol phosphate pathway. Biotechnol. Bioeng., 2020. [PUMA: myown]
Transcriptional Profiling of the Stringent Response Mutant Strain E. coli SR Reveals Enhanced Robustness to Large-Scale Conditions. microbial biotechnology, 2020. [PUMA: myown]
Methylthioadenosine (MTA) boosts cell-specific productivities of Chinese hamster ovary cultures: dosage effects on proliferation, cell cycle and gene expression. FEBS Open Bio, (10):2791-2804, 2020. [PUMA: myown]
Predicting By-Product Gradients of Baker’s Yeast Production at Industrial Scale: A Practical Simulation Approach. Processes, (8)2020. [PUMA: myown]
Streamlining the Analysis of Dynamic 13C-Labeling Patterns for the Metabolic Engineering of Corynebacterium glutamicum as l-Histidine Production Host. Metabolites, (10)4582020. [PUMA: myown]
Modeling Cell-Free Protein Synthesis Systems—Approaches and Applications. Frontiers in Bioengineering and Biotechnology, 2020. [PUMA: myown]
Data‐driven in silico prediction of regulation heterogeneity and ATP demands of Escherichia coli in large‐scale bioreactors. Biotechnol. Bioeng., (118)1:265-278, 2020. [PUMA: myown]
Revisiting the Growth Modulon of Corynebacterium glutamicum Under Glucose Limited Chemostat Conditions. Frontiers in Bioengineering and Biotechnology, 2020. [PUMA: myown]
S-adenosylmethionine and methylthioadenosine boost cellular productivities of antibody forming Chinese hamster ovary cells. Biotechnol. Bioeng., 2020. [PUMA: myown]
Electron availability in CO2, CO and H2 mixtures constrains flux distribution, energy management and product formation in Clostridium ljungdahlii. Microbial Biotechnology, 2020. [PUMA: myown]
Comparison of l-tyrosine containing dipeptides reveals maximum ATP availability for l-prolyl-l-tyrosine in CHO cells. Engineering in Life Science, 2020. [PUMA: myown]
Simulated oxygen and glucose gradients as a prerequisite for predicting industrial scale performance a priori. Biotechnol. Bioeng., (117)9:2760-2770, 2020. [PUMA: myown]
Engineering Pseudomonas putida KT2440 for the production of isobutanol. Engineering in Life Science, (20):148-159, 2019. [PUMA: myown]
Pseudomonas putida KT2440 is naturally endowed to withstand industrial-scale stress conditions. micobial biotechnology, 1-17, 2020. [PUMA: myown]
Reduced and Minimal Cell Factories in Bioprocesses: Towards a Streamlined Chassis. In: Minimal Cells: Design, Construction, Biotechnological Applications. In A.R. Lara, and G. Gosset Lagarda (Eds.), 2019. [PUMA: myown]
Biochemical engineering provides mindset, tools and solutions for the driving questions of a sustainable future. Engineering in Life Science, 2019. [PUMA: myown]
Synergistically Applying 1D modeling and CFD for Designing Industrial Scale Bubble Column Syngas Bioreactors. Engineering in Life Science, 2020. [PUMA: myown]
Prediction of novel non-coding RNAs relevant for the growth of Pseudomonas putida in a bioreactor. Microbiology, 2019. [PUMA: myown]
CO2/HCO3- accelerates iron reduction through phenolic compounds. mbio, 2020. [PUMA: myown]
Continuous Adaptive Evolution of a Fast-Growing Corynebacterium glutamicum Strain Independent of Protocatechuate. Frontiers in Microbiology, 2019. [PUMA: myown]
Exploting Hydrogenophage pseudoflava for aerobic syngas-based production of chemicals. Metabolic Engineering, (55):220-230, 2019. [PUMA: myown]
The impact of CO gradients on C. ljungdahlii in a 125 m³ bubble column: Mass transfer, circulation time and lifeline analysis. Chemical Engineering Science, (207):410-423, 2019. [PUMA: myown]
Identifying the Growth Modulon of Corynebacterium glutamicum. Frontiers in Microbiology, (10)9742019. [PUMA: myown]
Grand Research Challenges for Sustainable Industrial Biotechnology. Trends in Biotechnology, 2019. [PUMA: myown]
The less the better: How suppressed base addition boosts production of monoclonal antibodies with Chinese Hamster Ovary Cells. Frontiers in Bioengineering and Biotechnlogy, (7)762019. [PUMA: myown]
HILIC-Enabled 13C Metabolomics Strategies: Comparing Quantitative Precision and Spectral Accuracy of QToF High - and QQQ Low-Resolution Mass Spectrometry. Metabolites, 2019. [PUMA: myown]
Modular systems metabolic engineering enables balancing of relevant pathways for l-histidine production with Corynebacterium glutamicum. Biotechnology for Biofuels, (12)12019. [PUMA: myown]
From nutrional wealth to autophagy: In vivo metabolic dynamics in the cytosol, mitochondrion and shuttles of IgG producing CHO cells. Metabolic Engineering, (54):145-159, 2019. [PUMA: myown]
Metabolic engineering to guide evolution - Creating a novel mode for L-valine production with Corynebacterium glutamicum. Metabolic Engineering, (47):31-41, 2018. [PUMA: myown]
Protein production in Escherichia coli is guided by the trade‑off between intracellular substrate availability and energy cost. Microbial Cell Factories, (18)82019. [PUMA: myown]
Perfusion cultures equire optimum respiratory ATP supply to maximize cell‐specific and volumetric productivities. Biotechnol. Bioeng., 1-10, 2019. [PUMA: myown]
A guide to gene regulatory network interference for obtaining predictive solutions: Underlying assumptions and fundamental biological and data constraints. BioSystems, (174):37-48, 2018. [PUMA: myown]
Deciphering the Adaptation of Corynebacterium glutamicum in Transition from Aerobiosis via Microaerobiosis to Anaerobiosis. Genes, (9)6:297, 2018. [PUMA: myown] URL
Fed-Batch Production of recombinant human calcitonin precursor fusion-protein using Staphylococcus carnosus as expression-sectretion system. Appl. Microbiol. Biotechnol., (54):361-369, 2000. [PUMA: myown]
Human chymotrypsinogen B production with Pichia pastoris by integrated fermentation and downstream processing Part I: Fermentation processing. Biotechnol. Prog, (17):495-502, 2001. [PUMA: myown]
Human chymotrypsinogen B production with Pichia pastoris by integrated fermentation and downstream processing Part II: Protein recovery. Biotechnol. Prog, (17):503-512, 2001. [PUMA: myown]
Metabolomics: Quantification of intracellular metabolite dynamics. Biomol. Eng., (19)1:5-15, 2002. [PUMA: myown]
MMT – A Pathway Modeling Tool for Data From Rapid Sampling Experiments. In Silico Biology, (2)4:467-484, 2002. [PUMA: myown]
CO2 - Intrinsic Product, Essential Substrate, and Regulatory Trigger of Microbial and Mammalian Production Processes. Front Bioeng Biotechnol, (3):108, 2015. [PUMA: myown]
Dynamics of benzoate metabolism in Pseudomonas putida KT2440. Metabolic Engineering Communications, (3):97-110, 2016. [PUMA: myown]
Using gas mixtures of CO, CO2 and H2 as microbial substrates: the do's and don'ts of successful technology transfer from laboratory to production scale. Microbial Biotechnology, (11)42018. [PUMA: myown]
In Silico Prediction of Large-Scale Microbial Production Performance: Constraints for Getting Proper Data-Driven Models. Computational and Structural Biotechnology Journal, (16):246 - 256, 2018. [PUMA: myown] URL
Physiological Response of Corynebacterium glutamicum to Increasingly Nutrient-Rich Growth Conditions. Frontiers in Microbiology, (9)Frontiers Media SA, August 2018. [PUMA: myown] URL
Investigating the dynamic behavior of biochemical networks using model families. BIOINFORMATICS, (21)8:1617-1625, OXFORD UNIV PRESS, GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND, April 2005. [PUMA: myown] URL
Evolution of pyruvate kinase-deficient Escherichia coli mutants enables glycerol-based cell growth and succinate production. JOURNAL OF APPLIED MICROBIOLOGY, (115)6:1368-1378, WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA, December 2013. [PUMA: myown] URL
Improving the carbon balance of fermentations by total carbon analyses. BIOCHEMICAL ENGINEERING JOURNAL, (90):162-169, ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, September 2014. [PUMA: myown] URL
Production of 1-Octanol from n-Octane by Pseudomonas putida KT2440. CHEMIE INGENIEUR TECHNIK, (85)6:841-848, WILEY-V C H VERLAG GMBH, BOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANY, June 2013. [PUMA: myown] URL
Impact of different CO2/HCO3- levels on metabolism and regulation in Corynebacterium glutamicum. JOURNAL OF BIOTECHNOLOGY, (168)4:331-340, ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, December 2013. [PUMA: myown] URL
Platform Engineering of Corynebacterium glutamicum with Reduced Pyruvate Dehydrogenase Complex Activity for Improved Production of L-Lysine, L-Valine, and 2-Ketoisovalerate. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, (79)18:5566-5575, AMER SOC MICROBIOLOGY, 1752 N ST NW, WASHINGTON, DC 20036-2904 USA, September 2013. [PUMA: myown] URL
Genome reduction boosts heterologous gene expression in Pseudomonas putida. MICROBIAL CELL FACTORIES, (14)BIOMED CENTRAL LTD, 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND, February 2015. [PUMA: myown] URL
Compartment-specific metabolomics for CHO reveals that ATP pools in mitochondria are much lower than in cytosol. BIOTECHNOLOGY JOURNAL, (10)10, SI:1639-1650, WILEY-V C H VERLAG GMBH, POSTFACH 101161, 69451 WEINHEIM, GERMANY, October 2015. [PUMA: myown] URL
Monitoring intracellular protein degradation in antibody-producing Chinese hamster ovary cells. ENGINEERING IN LIFE SCIENCES, (15)5, SI:499-508, WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA, July 2015. [PUMA: myown] URL
Applying systems biology tools to study n-butanol degradation in Pseudomonas putida KT2440. ENGINEERING IN LIFE SCIENCES, (15)8:760-771, WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA, November 2015. [PUMA: myown] URL
Alkaline conditions in hydrophilic interaction liquid chromatography for intracellular metabolite quantification using tandem mass spectrometry. ANALYTICAL BIOCHEMISTRY, (475):4-13, ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA, April 2015. [PUMA: myown] URL
Changes in intracellular ATP-content of CHO cells as response to hyperosmolality. BIOTECHNOLOGY PROGRESS, (31)5:1212-1216, WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA, September 2015. [PUMA: myown] URL
Editorial: How can we ensure the successful transfer from lab- to large-scale production?. ENGINEERING IN LIFE SCIENCES, (16)7:587, WILEY, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA, October 2016. [PUMA: myown] URL
LC-MS-based compartment-specific metabolome analysis in CHO. NEW BIOTECHNOLOGY, (33)S:S33-S34, ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, July 2016. [PUMA: myown] URL
Editorial overview: Microbial systems biology: systems biology prepares the ground for successful synthetic biology. CURRENT OPINION IN MICROBIOLOGY, (33):VIII-X, CURRENT BIOLOGY LTD, 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND, October 2016. [PUMA: myown] URL
Environmental stress speeds up DNA replication in Pseudomonas putida in chemostat cultivations. BIOTECHNOLOGY JOURNAL, (11)1, SI:155-163, WILEY-V C H VERLAG GMBH, POSTFACH 101161, 69451 WEINHEIM, GERMANY, January 2016. [PUMA: myown] URL
Predictability of k(L)a in stirred tank reactors under multiple operating conditions using an Euler-Lagrange approach. ENGINEERING IN LIFE SCIENCES, (16)7:633-642, WILEY, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA, October 2016. [PUMA: myown] URL
Hyperosmotic stimulus study discloses benefits in ATP supply and reveals miRNA/mRNA targets to improve recombinant protein production of CHO cells. BIOTECHNOLOGY JOURNAL, (11)8:1037-1047, WILEY-V C H VERLAG GMBH, POSTFACH 101161, 69451 WEINHEIM, GERMANY, August 2016. [PUMA: myown] URL
Tracking dipeptides at work-uptake and intracellular fate in CHO culture. AMB EXPRESS, (6)BIOMED CENTRAL LTD, 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND, July 2016. [PUMA: myown] URL
Experimentally Validated Model Enables Debottlenecking of in Vitro Protein Synthesis and Identifies a Control Shift under in Vivo Conditions. ACS SYNTHETIC BIOLOGY, (6)10:1913-1921, AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA, October 2017. [PUMA: myown] URL
Zero-growth bioprocesses: A challenge for microbial production strains and bioprocess engineering. ENGINEERING IN LIFE SCIENCES, (17)1:27-35, WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA, January 2017. [PUMA: myown] URL