%0 Journal Article %1 Dobslaw.2012 %A Dobslaw, Daniel %A Engesser, Karl-Heinrich %D 2012 %J Applied microbiology and biotechnology %K alr dobslaw engesser iswa %N 5 %P 2205--2214 %R 10.1007/s00253-011-3543-5 %T Degradation of 2-chlorotoluene by Rhodococcus sp. OCT 10 %V 93 %X A strain Rhodococcus sp. OCT 10 DSM 45596(T), exhibiting 99.9\% of 16S rDNA identity with Rhodococcus wratislaviensis NCIMB 13082, was isolated from a soil sample. The strain completely mineralised 2-chlorotoluene, 2-bromotoluene, o-xylene, benzyl alcohol and benzoate. In contrast, 2-fluorotoluene was only partially mineralised. By GC-MS and (1)H-NMR analyses, 4-chloro-3-methylcatechol was identified as the central intermediate in the degradation pathway of 2-chlorotoluene. It was further degraded by enzymes of the meta cleavage pathway. Catechol 1,2-dioxygenase and chlorocatechol 1,2-dioxygenase as the initial enzymes of the ortho cleavage pathways were not detectable under these conditions. Furthermore, neither formation nor oxidation of 2-chlorobenzylic alcohol, 2-chlorobenzaldehyde, or 2-chlorobenzoate was observed, thereby excluding side chain oxidation activity.

@article{Dobslaw.2012, abstract = {A strain Rhodococcus sp. OCT 10 DSM 45596(T), exhibiting 99.9{\%} of 16S rDNA identity with Rhodococcus wratislaviensis NCIMB 13082, was isolated from a soil sample. The strain completely mineralised 2-chlorotoluene, 2-bromotoluene, o-xylene, benzyl alcohol and benzoate. In contrast, 2-fluorotoluene was only partially mineralised. By GC-MS and (1)H-NMR analyses, 4-chloro-3-methylcatechol was identified as the central intermediate in the degradation pathway of 2-chlorotoluene. It was further degraded by enzymes of the meta cleavage pathway. Catechol 1,2-dioxygenase and chlorocatechol 1,2-dioxygenase as the initial enzymes of the ortho cleavage pathways were not detectable under these conditions. Furthermore, neither formation nor oxidation of 2-chlorobenzylic alcohol, 2-chlorobenzaldehyde, or 2-chlorobenzoate was observed, thereby excluding side chain oxidation activity.}, added-at = {2018-09-05T13:52:51.000+0200}, author = {Dobslaw, Daniel and Engesser, Karl-Heinrich}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/23da76ed027216c899fc3b9c95b40b09d/steffenhelbich}, doi = {10.1007/s00253-011-3543-5}, interhash = {dc1519d87cb4334126c873c25bc009a6}, intrahash = {3da76ed027216c899fc3b9c95b40b09d}, journal = {Applied microbiology and biotechnology}, keywords = {alr dobslaw engesser iswa}, number = 5, pages = {2205--2214}, timestamp = {2018-09-05T12:00:12.000+0200}, title = {Degradation of 2-chlorotoluene by Rhodococcus sp. OCT 10}, volume = 93, year = 2012 }