%0 Journal Article %1 hellwege2000 %A Hellwege, Elke M. %A Czapla, Sylvia %A Jahnke, Anuschka %A Willmitzer, Lothar %A Heyer, Arnd G. %B 15 %D 2000 %J Proceedings of the National Academy of Sciences %K metabolism myown %P 8699-8704 %R 10.1073/pnas.150043797 %T Transgenic potato (Solanum tuberosum) tubers synthesize the full spectrum of inulin molecules naturally occurring in globe artichoke (Cynara scolymus) roots %U http://www.pnas.org/content/pnas/97/15/8699.full.pdf %V 97 %X The ability to synthesize high molecular weight inulin was transferred to potato plants via constitutive expression of the 1-SST (sucrose:sucrose 1-fructosyltransferase) and the 1-FFT (fructan: fructan 1-fructosyltransferase) genes of globe artichoke (Cynara scolymus). The fructan pattern of tubers from transgenic potato plants represents the full spectrum of inulin molecules present in artichoke roots as shown by high-performance anion exchange chromatography, as well as size exclusion chromatography. These results demonstrate in planta that the enzymes sucrose:sucrose 1-fructosyltransferase and fructan:fructan 1-fructosyltransferase are sufficient to synthesize inulin molecules of all chain lengths naturally occurring in a given plant species. Inulin made up 5% of the dry weight of transgenic tubers, and a low level of fructan production also was observed in fully expanded leaves. Although inulin accumulation did not influence the sucrose concentration in leaves or tubers, a reduction in starch content occurred in transgenic tubers, indicating that inulin synthesis did not increase the storage capacity of the tubers. 1-SST,sucrose:sucrose 1-fructosyltransferase;1-FFT,fructan:fructan 1-fructosyltransferase;HPAEC,high-performance anion exchange chromatography;SEC,size exclusion chromatography;DP,degree of polymerization

@article{hellwege2000, abstract = {The ability to synthesize high molecular weight inulin was transferred to potato plants via constitutive expression of the 1-SST (sucrose:sucrose 1-fructosyltransferase) and the 1-FFT (fructan: fructan 1-fructosyltransferase) genes of globe artichoke (Cynara scolymus). The fructan pattern of tubers from transgenic potato plants represents the full spectrum of inulin molecules present in artichoke roots as shown by high-performance anion exchange chromatography, as well as size exclusion chromatography. These results demonstrate in planta that the enzymes sucrose:sucrose 1-fructosyltransferase and fructan:fructan 1-fructosyltransferase are sufficient to synthesize inulin molecules of all chain lengths naturally occurring in a given plant species. Inulin made up 5% of the dry weight of transgenic tubers, and a low level of fructan production also was observed in fully expanded leaves. Although inulin accumulation did not influence the sucrose concentration in leaves or tubers, a reduction in starch content occurred in transgenic tubers, indicating that inulin synthesis did not increase the storage capacity of the tubers. 1-SST,sucrose:sucrose 1-fructosyltransferase;1-FFT,fructan:fructan 1-fructosyltransferase;HPAEC,high-performance anion exchange chromatography;SEC,size exclusion chromatography;DP,degree of polymerization }, added-at = {2018-09-11T11:57:09.000+0200}, author = {Hellwege, Elke M. and Czapla, Sylvia and Jahnke, Anuschka and Willmitzer, Lothar and Heyer, Arnd G.}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/224e591e957325b750aa0606bf2b8d4da/arndheyer}, doi = {10.1073/pnas.150043797}, interhash = {90303992eb882e6a8ad47719c4c2afd0}, intrahash = {24e591e957325b750aa0606bf2b8d4da}, journal = {Proceedings of the National Academy of Sciences}, keywords = {metabolism myown}, pages = {8699-8704}, series = 15, timestamp = {2021-06-11T14:16:39.000+0200}, title = {Transgenic potato (Solanum tuberosum) tubers synthesize the full spectrum of inulin molecules naturally occurring in globe artichoke (Cynara scolymus) roots}, url = {http://www.pnas.org/content/pnas/97/15/8699.full.pdf}, volume = 97, year = 2000 }