Abstract
Ribosomes are a crucial component of the physiological state of a cell.
Therefore, we aimed to monitor ribosome dynamics using a fast and easy
fluorescence readout. Using fluorescent-labeled ribosomal proteins, the
dynamics of ribosomes during batch cultivation and during nutritional
shift conditions was investigated. The fluorescence readout was compared
to the cellular rRNA content determined by capillary gel electrophoresis
with laser-induced fluorescence detection during exponentially
accelerating and decelerating growth. We found a linear correlation
between the observed fluorescence and the extracted rRNA content
throughout cultivation, demonstrating the applicability of this method.
Moreover, the results show that ribosome dynamics, as a result of
slowing growth, are accompanied by the passive effect of dilution of
preexisting ribosomes, de novo ribosome synthesis and ribosome
degradation. In light of the challenging task of deciphering ribosome
regulatory mechanisms, our approach of using fluorescence to follow
ribosome dynamics will allow more comprehensive studies of biological
systems.
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