Carbon dioxide formation mirrors the final carbon oxidation steps of aerobic metabolism in microbial and mammalian cells. As a consequence, Formula: see text dissociation equilibria arise in fermenters by the growing culture. Anaplerotic reactions make use of the abundant Formula: see text levels for refueling citric acid cycle demands and for enabling oxaloacetate-derived products. At the same time, CO2 is released manifold in metabolic reactions via decarboxylation activity. The levels of extracellular Formula: see text depend on cellular activities and physical constraints such as hydrostatic pressures, aeration, and the efficiency of mixing in large-scale bioreactors. Besides, local Formula: see text levels might also act as metabolic inhibitors or transcriptional effectors triggering regulatory events inside the cells. This review gives an overview about fundamental physicochemical properties of Formula: see text in microbial and mammalian cultures effecting cellular physiology, production processes, metabolic activity, and transcriptional regulation.
%0 Journal Article
%1 blombach_co2_2015
%A Blombach, Bastian
%A Takors, Ralf
%D 2015
%J Front Bioeng Biotechnol
%K bicarbonate, carbon carboxylation, decarboxylation dioxide, myown process, production regulation,
%P 108
%R 10.3389/fbioe.2015.00108
%T CO2 - Intrinsic Product, Essential Substrate, and Regulatory Trigger of Microbial and Mammalian Production Processes
%V 3
%X Carbon dioxide formation mirrors the final carbon oxidation steps of aerobic metabolism in microbial and mammalian cells. As a consequence, Formula: see text dissociation equilibria arise in fermenters by the growing culture. Anaplerotic reactions make use of the abundant Formula: see text levels for refueling citric acid cycle demands and for enabling oxaloacetate-derived products. At the same time, CO2 is released manifold in metabolic reactions via decarboxylation activity. The levels of extracellular Formula: see text depend on cellular activities and physical constraints such as hydrostatic pressures, aeration, and the efficiency of mixing in large-scale bioreactors. Besides, local Formula: see text levels might also act as metabolic inhibitors or transcriptional effectors triggering regulatory events inside the cells. This review gives an overview about fundamental physicochemical properties of Formula: see text in microbial and mammalian cultures effecting cellular physiology, production processes, metabolic activity, and transcriptional regulation.
@article{blombach_co2_2015,
abstract = {Carbon dioxide formation mirrors the final carbon oxidation steps of aerobic metabolism in microbial and mammalian cells. As a consequence, [Formula: see text] dissociation equilibria arise in fermenters by the growing culture. Anaplerotic reactions make use of the abundant [Formula: see text] levels for refueling citric acid cycle demands and for enabling oxaloacetate-derived products. At the same time, CO2 is released manifold in metabolic reactions via decarboxylation activity. The levels of extracellular [Formula: see text] depend on cellular activities and physical constraints such as hydrostatic pressures, aeration, and the efficiency of mixing in large-scale bioreactors. Besides, local [Formula: see text] levels might also act as metabolic inhibitors or transcriptional effectors triggering regulatory events inside the cells. This review gives an overview about fundamental physicochemical properties of [Formula: see text] in microbial and mammalian cultures effecting cellular physiology, production processes, metabolic activity, and transcriptional regulation.},
added-at = {2018-02-09T13:18:17.000+0100},
author = {Blombach, Bastian and Takors, Ralf},
biburl = {https://puma.ub.uni-stuttgart.de/bibtex/2ef8131870fb5db07e873c177cfee4609/bastian},
doi = {10.3389/fbioe.2015.00108},
interhash = {843b15643d0c3ef44a9efb1e4d337222},
intrahash = {ef8131870fb5db07e873c177cfee4609},
issn = {2296-4185},
journal = {Front Bioeng Biotechnol},
keywords = {bicarbonate, carbon carboxylation, decarboxylation dioxide, myown process, production regulation,},
language = {eng},
pages = 108,
pmcid = {PMC4522908},
pmid = {26284242},
timestamp = {2018-02-09T12:18:56.000+0100},
title = {{CO}2 - {Intrinsic} {Product}, {Essential} {Substrate}, and {Regulatory} {Trigger} of {Microbial} and {Mammalian} {Production} {Processes}},
volume = 3,
year = 2015
}