The pyruvate dehydrogenase complex (PDHC) catalyzes the oxidative thiamine pyrophosphate-dependent decarboxylation of pyruvate to acetyl-CoA and CO2. Since pyruvate is a key metabolite of the central metabolism and also the precursor for several relevant biotechnological products, metabolic engineering of this multienzyme complex is a promising strategy to improve microbial production processes. This review summarizes the current knowledge and achievements on metabolic engineering approaches to tailor the PDHC of Corynebacterium glutamicum for the bio-based production of l-valine, 2-ketosiovalerate, pyruvate, succinate and isobutanol and to improve l-lysine production.
%0 Journal Article
%1 eikmanns_pyruvate_2014
%A Eikmanns, Bernhard J.
%A Blombach, Bastian
%D 2014
%J J. Biotechnol.
%K Acid, Amino Butanols, Complex, Corynebacterium Dehydrogenase Engineering, Isobutanol Metabolic Pyruvate Pyruvic Valine, acid and complex dehydrogenase engineering, glutamicum, myown organic production,
%P 339--345
%R 10.1016/j.jbiotec.2013.12.019
%T The pyruvate dehydrogenase complex of Corynebacterium glutamicum: an attractive target for metabolic engineering
%V 192 Pt B
%X The pyruvate dehydrogenase complex (PDHC) catalyzes the oxidative thiamine pyrophosphate-dependent decarboxylation of pyruvate to acetyl-CoA and CO2. Since pyruvate is a key metabolite of the central metabolism and also the precursor for several relevant biotechnological products, metabolic engineering of this multienzyme complex is a promising strategy to improve microbial production processes. This review summarizes the current knowledge and achievements on metabolic engineering approaches to tailor the PDHC of Corynebacterium glutamicum for the bio-based production of l-valine, 2-ketosiovalerate, pyruvate, succinate and isobutanol and to improve l-lysine production.
@article{eikmanns_pyruvate_2014,
abstract = {The pyruvate dehydrogenase complex (PDHC) catalyzes the oxidative thiamine pyrophosphate-dependent decarboxylation of pyruvate to acetyl-CoA and CO2. Since pyruvate is a key metabolite of the central metabolism and also the precursor for several relevant biotechnological products, metabolic engineering of this multienzyme complex is a promising strategy to improve microbial production processes. This review summarizes the current knowledge and achievements on metabolic engineering approaches to tailor the PDHC of Corynebacterium glutamicum for the bio-based production of l-valine, 2-ketosiovalerate, pyruvate, succinate and isobutanol and to improve l-lysine production.},
added-at = {2018-02-09T13:18:17.000+0100},
author = {Eikmanns, Bernhard J. and Blombach, Bastian},
biburl = {https://puma.ub.uni-stuttgart.de/bibtex/2ed56a8ffa25fcb7fe239b276b7e52493/bastian},
doi = {10.1016/j.jbiotec.2013.12.019},
interhash = {11bc42d6baae0b586acdbed655538eb6},
intrahash = {ed56a8ffa25fcb7fe239b276b7e52493},
issn = {1873-4863},
journal = {J. Biotechnol.},
keywords = {Acid, Amino Butanols, Complex, Corynebacterium Dehydrogenase Engineering, Isobutanol Metabolic Pyruvate Pyruvic Valine, acid and complex dehydrogenase engineering, glutamicum, myown organic production,},
language = {eng},
month = dec,
pages = {339--345},
pmid = {24486441},
shorttitle = {The pyruvate dehydrogenase complex of {Corynebacterium} glutamicum},
timestamp = {2018-02-09T12:18:56.000+0100},
title = {The pyruvate dehydrogenase complex of {Corynebacterium} glutamicum: an attractive target for metabolic engineering},
volume = {192 Pt B},
year = 2014
}