%0 Generic %1 deggen2023supplementary %A Degen, Morris %A Santos, José Carlos %A Pluhackova, Kristyna %A Cebrero, Gonzalo %A Ramos, Saray %A Jankevicius, Gytis %A Hartenian, Ella %A Guillerm, Undina %A Mari, Stefania A. %A Kohl, Bastian %A Müller, Daniel J. %A Schanda, Paul %A Maier, Timm %A Perez, Camilo %A Sieben, Christian %A Broz, Petr %A Hiller, Sebastian %D 2023 %K darus ubs_10021 ubs_20019 ubs_30165 unibibliografie %R 10.18419/darus-3373 %T Supplementary material for 'Structural basis for ninjurin-1 mediated plasma membrane rupture in lytic cell death' %X Eukaryotic cells can undergo different forms of programmed cell death, many of which culminate in plasma membrane rupture (PMR) as the defining terminal event. PMR was long thought to be driven by osmotic pressure, until it was recently shown to be in many cases an active process, mediated by the protein ninjurin-1 (NINJ1). Here, we studied whether the newly resolved cryo-electron microscopy NINJ1 filaments are capable to cap edges of membrane edges by multiscaling molecular dynamics simulations. Site-directed mutagenesis was employed to validate the function of the resulting supramolecular arrangement. The membrane protein NINJ1 is thus an interactive component of the eukaryotic cell membrane that functions as an in-built breaking point in response to activation of cell death.

@misc{deggen2023supplementary, abstract = {Eukaryotic cells can undergo different forms of programmed cell death, many of which culminate in plasma membrane rupture (PMR) as the defining terminal event. PMR was long thought to be driven by osmotic pressure, until it was recently shown to be in many cases an active process, mediated by the protein ninjurin-1 (NINJ1). Here, we studied whether the newly resolved cryo-electron microscopy NINJ1 filaments are capable to cap edges of membrane edges by multiscaling molecular dynamics simulations. Site-directed mutagenesis was employed to validate the function of the resulting supramolecular arrangement. The membrane protein NINJ1 is thus an interactive component of the eukaryotic cell membrane that functions as an in-built breaking point in response to activation of cell death. }, added-at = {2023-03-13T08:41:26.000+0100}, affiliation = {Degen, Morris/Biozentrum, University of Basel, Santos, José Carlos/Department of Immunobiology, University of Lausanne, Pluhackova, Kristyna/SC SimTech, University of Stuttgart, Cebrero, Gonzalo/Biozentrum, University of Basel, Ramos, Saray/Department of Immunobiology, University of Lausanne, Jankevicius, Gytis/Biozentrum, University of Basel, Hartenian, Ella/Department of Immunobiology, University of Lausanne, Guillerm, Undina/Institute of Science and Technology Austria, Mari, Stefania A./Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich, Kohl, Bastian/Biozentrum, University of Basel, Müller, Daniel J./Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule (ETH) Zurich, Schanda, Paul/Institute of Science and Technology Austria, Maier, Timm/Biozentrum, University of Basel, Perez, Camilo/Biozentrum, University of Basel, Sieben, Christian/Institute for Genetics, Technische Universität Braunschweig; Nanoscale Infection Biology Group, Department of Cell Biology, Helmholtz Centre for Infection Research, Broz, Petr/Department of Immunobiology, University of Lausanne, Hiller, Sebastian/Biozentrum, University of Basel}, author = {Degen, Morris and Santos, José Carlos and Pluhackova, Kristyna and Cebrero, Gonzalo and Ramos, Saray and Jankevicius, Gytis and Hartenian, Ella and Guillerm, Undina and Mari, Stefania A. and Kohl, Bastian and Müller, Daniel J. and Schanda, Paul and Maier, Timm and Perez, Camilo and Sieben, Christian and Broz, Petr and Hiller, Sebastian}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/29e1919d426fbf9a1855719f7e5c97d7c/unibiblio}, doi = {10.18419/darus-3373}, howpublished = {Dataset}, interhash = {8d5bec6a657e2e476700c8f48e7c195b}, intrahash = {9e1919d426fbf9a1855719f7e5c97d7c}, keywords = {darus ubs_10021 ubs_20019 ubs_30165 unibibliografie}, note = {Related to: Degen, Morris; Santos, José Carlos; Pluhackova, Kristyna; Cebrero, Gonzalo; Ramos, Saray; Jankevicius, Gytis; Hartenian, Ella; Guillerm, Undina; Mari, Stefania A.; Kohl, Bastian; Müller, Daniel J.; Schanda, Paul; Maier, Timm; Perez, Camilo; Sieben, Christian; Broz, Petr; Hiller, Sebastian, "Structural basis for ninjurin-1 mediated plasma membrane rupture in lytic cell death", Nature 2023. doi: 10.1038/s41586-023-05991-z}, orcid-numbers = {Degen, Morris/0000-0003-3762-5779, Santos, José Carlos/0000-0002-3446-4001, Pluhackova, Kristyna/0000-0002-7498-6584, Cebrero, Gonzalo/0000-0001-7171-0921, Ramos, Saray/0000-0001-6015-9310, Jankevicius, Gytis/0000-0002-0212-3923, Hartenian, Ella/0000-0001-9277-2152, Mari, Stefania A./0000-0002-6420-8578, Kohl, Bastian/0000-0001-6601-8340, Müller, Daniel J./0000-0003-3075-0665, Schanda, Paul/0000-0002-9350-7606, Maier, Timm/0000-0002-7459-1363, Perez, Camilo/0000-0002-5800-0084, Sieben, Christian/0000-0002-2836-2623, Broz, Petr/0000-0002-2334-7790, Hiller, Sebastian/0000-0002-6709-4684}, timestamp = {2023-06-05T07:20:03.000+0200}, title = {Supplementary material for 'Structural basis for ninjurin-1 mediated plasma membrane rupture in lytic cell death'}, year = 2023 }