This article outlines the new non-contact TOPSPOT method for printing microarrays in a highly parallelized fashion. It is based on a micromachined print head incorporating a central microarray of presently up to 96 vertical nozzles on the output side. Droplets featuring volumes down to 1nl aligned in a 500 ?m grid are simultaneously ejected by applying a steep air pressure ramp to the open upper side of the liquid. Each of these nozzles is connected to a distinct fluidic reservoir constituting the interface to the macro world. To allow an automated replenishing of the chip, the alignment of the reservoirs is amenable for liquid handling via standard pipetting robots. Depending on the design of the print head, a maximum of 20 ?l can be stored thus allowing one to dispend about 20.000 droplets of equal quality in a row. Besides its suitability for a robust biochip manufacturing facility in the laboratory, the print head is also well-integrable in a high-throughput production plant. These versions are scheduled to become commercially available in 2000
%0 Conference Paper
%1 gruhler2000topspot
%A Gruhler, H.
%A Hey, N.
%A Müller, M.
%A Bekesi, S.
%A Freygang, M.
%A Sandmaier, Hermann
%A Zengerle, Roland
%B Screentech 2000
%C Southborough, MA
%D 2000
%I International Business Communications
%K fis liste ubs_30119
%T TopSpot - A New Method for the Fabrication of BioChips
%X This article outlines the new non-contact TOPSPOT method for printing microarrays in a highly parallelized fashion. It is based on a micromachined print head incorporating a central microarray of presently up to 96 vertical nozzles on the output side. Droplets featuring volumes down to 1nl aligned in a 500 ?m grid are simultaneously ejected by applying a steep air pressure ramp to the open upper side of the liquid. Each of these nozzles is connected to a distinct fluidic reservoir constituting the interface to the macro world. To allow an automated replenishing of the chip, the alignment of the reservoirs is amenable for liquid handling via standard pipetting robots. Depending on the design of the print head, a maximum of 20 ?l can be stored thus allowing one to dispend about 20.000 droplets of equal quality in a row. Besides its suitability for a robust biochip manufacturing facility in the laboratory, the print head is also well-integrable in a high-throughput production plant. These versions are scheduled to become commercially available in 2000
@inproceedings{gruhler2000topspot,
abstract = {This article outlines the new non-contact TOPSPOT method for printing microarrays in a highly parallelized fashion. It is based on a micromachined print head incorporating a central microarray of presently up to 96 vertical nozzles on the output side. Droplets featuring volumes down to 1nl aligned in a 500 ?m grid are simultaneously ejected by applying a steep air pressure ramp to the open upper side of the liquid. Each of these nozzles is connected to a distinct fluidic reservoir constituting the interface to the macro world. To allow an automated replenishing of the chip, the alignment of the reservoirs is amenable for liquid handling via standard pipetting robots. Depending on the design of the print head, a maximum of 20 ?l can be stored thus allowing one to dispend about 20.000 droplets of equal quality in a row. Besides its suitability for a robust biochip manufacturing facility in the laboratory, the print head is also well-integrable in a high-throughput production plant. These versions are scheduled to become commercially available in 2000},
added-at = {2023-08-21T15:14:23.000+0200},
address = {Southborough, MA},
author = {Gruhler, H. and Hey, N. and Müller, M. and Bekesi, S. and Freygang, M. and Sandmaier, Hermann and Zengerle, Roland},
biburl = {https://puma.ub.uni-stuttgart.de/bibtex/276199b4acc4a77c7e40bc444e2be124c/unibiblio-4},
booktitle = {Screentech 2000},
eventdate = {2000-02-28/2000-03-03},
eventtitle = {Screentech 2000},
interhash = {36fd9a76441a9323c8dc87bf687e7315},
intrahash = {76199b4acc4a77c7e40bc444e2be124c},
keywords = {fis liste ubs_30119},
language = {eng},
publisher = {International Business Communications},
timestamp = {2023-09-01T10:41:40.000+0200},
title = {TopSpot - A New Method for the Fabrication of BioChips},
venue = {Monterey, USA},
year = 2000
}
