Regulatory RNA plays a pivotal role in the regulation of bacterial gene expression. Here, five small RNAs were studied in Sinorhizobium meliloti - SmrC15, SmrC16, Sra33, 6S and the signal recognition particle (SRP) RNA, which are conserved among at least seven different Rhizobium and Sinorhizobium species. The amount of SmrC16 decreased in stationary phase, while the other RNAs were up-regulated. The smallest changes, maximally 2-fold, were observed for 6S RNA. In the distantly related Bradyrhizobium japonicum, the amount of 6S RNA was not increased in stationary phase, suggesting some functional divergence in the roles of this molecule in Rhizobiales in comparison to Escherichia coli. Different decay rates were observed for SmrC15 and SmrC16 of S. meliloti upon rifampicin treatment, revealing posttranscriptional regulation during growth. The use of a Deltahfq mutant showed that Hfq protects full-length SmrC16 from degradation and stabilises its specific degradation products.
Biochemical and Biophysical Research Communications
number
2
pages
331--336
volume
390
urldate
2010-02-24
issn
0006-291X
file
ScienceDirect Snapshot:/Users/bvoss/Library/Application Support/Firefox/Profiles/a6kceokb.default/zotero/storage/ZH2WHJ8A/science.html:text/html;Voss et al. - 2009 - Expression of small RNAs in Rhizobiales and protec.pdf:/Users/bvoss/Library/Application Support/Firefox/Profiles/a6kceokb.default/zotero/storage/FTDWG524/Voss et al. - 2009 - Expression of small RNAs in Rhizobiales and protec.pdf:application/pdf
%0 Journal Article
%1 vos_expression_2009
%A Voß, Björn
%A Hölscher, Marion
%A Baumgarth, Birgit
%A Kalbfleisch, Andreas
%A Kaya, Ceren
%A Hess, Wolfgang R.
%A Becker, Anke
%A Elena Evguenieva-Hackenberg,
%D 2009
%J Biochemical and Biophysical Research Communications
%K 6S Bradyrhizobium, Hfq, RNA RNA, Sinorhizobium, Small Stability, myown
%N 2
%P 331--336
%R 10.1016/j.bbrc.2009.09.125
%T Expression of small RNAs in Rhizobiales and protection of a small RNA and its degradation products by Hfq in Sinorhizobium meliloti
%U http://www.sciencedirect.com/science/article/B6WBK-4XC57DX-2/2/32de0bcdf98272e9a9b196b95327e646
%V 390
%X Regulatory RNA plays a pivotal role in the regulation of bacterial gene expression. Here, five small RNAs were studied in Sinorhizobium meliloti - SmrC15, SmrC16, Sra33, 6S and the signal recognition particle (SRP) RNA, which are conserved among at least seven different Rhizobium and Sinorhizobium species. The amount of SmrC16 decreased in stationary phase, while the other RNAs were up-regulated. The smallest changes, maximally 2-fold, were observed for 6S RNA. In the distantly related Bradyrhizobium japonicum, the amount of 6S RNA was not increased in stationary phase, suggesting some functional divergence in the roles of this molecule in Rhizobiales in comparison to Escherichia coli. Different decay rates were observed for SmrC15 and SmrC16 of S. meliloti upon rifampicin treatment, revealing posttranscriptional regulation during growth. The use of a Deltahfq mutant showed that Hfq protects full-length SmrC16 from degradation and stabilises its specific degradation products.
@article{vos_expression_2009,
abstract = {Regulatory RNA plays a pivotal role in the regulation of bacterial gene expression. Here, five small RNAs were studied in Sinorhizobium meliloti - SmrC15, SmrC16, Sra33, 6S and the signal recognition particle (SRP) RNA, which are conserved among at least seven different Rhizobium and Sinorhizobium species. The amount of SmrC16 decreased in stationary phase, while the other RNAs were up-regulated. The smallest changes, maximally 2-fold, were observed for 6S RNA. In the distantly related Bradyrhizobium japonicum, the amount of 6S RNA was not increased in stationary phase, suggesting some functional divergence in the roles of this molecule in Rhizobiales in comparison to Escherichia coli. Different decay rates were observed for SmrC15 and SmrC16 of S. meliloti upon rifampicin treatment, revealing posttranscriptional regulation during growth. The use of a [Delta]hfq mutant showed that Hfq protects full-length SmrC16 from degradation and stabilises its specific degradation products.},
added-at = {2016-08-23T12:53:21.000+0200},
author = {Voß, Björn and Hölscher, Marion and Baumgarth, Birgit and Kalbfleisch, Andreas and Kaya, Ceren and Hess, Wolfgang R. and Becker, Anke and {Elena Evguenieva-Hackenberg}},
biburl = {https://puma.ub.uni-stuttgart.de/bibtex/249e317586f9d46a55f49003358cb45d2/bvoss},
doi = {10.1016/j.bbrc.2009.09.125},
file = {ScienceDirect Snapshot:/Users/bvoss/Library/Application Support/Firefox/Profiles/a6kceokb.default/zotero/storage/ZH2WHJ8A/science.html:text/html;Voss et al. - 2009 - Expression of small RNAs in Rhizobiales and protec.pdf:/Users/bvoss/Library/Application Support/Firefox/Profiles/a6kceokb.default/zotero/storage/FTDWG524/Voss et al. - 2009 - Expression of small RNAs in Rhizobiales and protec.pdf:application/pdf},
interhash = {95a7bbfdf63e8c34e49de855a58e9d35},
intrahash = {49e317586f9d46a55f49003358cb45d2},
issn = {0006-291X},
journal = {Biochemical and Biophysical Research Communications},
keywords = {6S Bradyrhizobium, Hfq, RNA RNA, Sinorhizobium, Small Stability, myown},
note = 00000,
number = 2,
pages = {331--336},
timestamp = {2016-08-23T12:11:23.000+0200},
title = {Expression of small {RNAs} in {Rhizobiales} and protection of a small {RNA} and its degradation products by {Hfq} in {Sinorhizobium} meliloti},
url = {http://www.sciencedirect.com/science/article/B6WBK-4XC57DX-2/2/32de0bcdf98272e9a9b196b95327e646},
urldate = {2010-02-24},
volume = 390,
year = 2009
}