We isolated Gram-positive alkane-degraders from soil and a tricking-bed reactor, and show using polymerase chain reaction (PCR) with degenerate alkane hydroxylase primers and Southern blots that most Rhodococcus isolates contain three to five quite divergent homologues of the Pseudomonas putida GPo1 alkB gene. Two Mycobacterium isolates each contain one homologue, however there is no evidence for the presence of alkB homologues in the remaining strains.
%0 Journal Article
%1 vanBeilen.2002
%A van Beilen, Jan B.
%A Smits, Theo H. M.
%A Whyte, Lyle G.
%A Schorcht, Susanne
%A Röthlisberger, Martina
%A Plaggemeier, Thorsten
%A Engesser, Karl-Heinrich
%A Witholt, Bernard
%D 2002
%J Environmental microbiology
%K alr engesser iswa
%N 11
%P 676--682
%T Alkane hydroxylase homologues in Gram-positive strains
%V 4
%X We isolated Gram-positive alkane-degraders from soil and a tricking-bed reactor, and show using polymerase chain reaction (PCR) with degenerate alkane hydroxylase primers and Southern blots that most Rhodococcus isolates contain three to five quite divergent homologues of the Pseudomonas putida GPo1 alkB gene. Two Mycobacterium isolates each contain one homologue, however there is no evidence for the presence of alkB homologues in the remaining strains.
@article{vanBeilen.2002,
abstract = {We isolated Gram-positive alkane-degraders from soil and a tricking-bed reactor, and show using polymerase chain reaction (PCR) with degenerate alkane hydroxylase primers and Southern blots that most Rhodococcus isolates contain three to five quite divergent homologues of the Pseudomonas putida GPo1 alkB gene. Two Mycobacterium isolates each contain one homologue, however there is no evidence for the presence of alkB homologues in the remaining strains.},
added-at = {2018-09-05T13:52:51.000+0200},
author = {{van Beilen}, Jan B. and Smits, Theo H. M. and Whyte, Lyle G. and Schorcht, Susanne and R{\"o}thlisberger, Martina and Plaggemeier, Thorsten and Engesser, Karl-Heinrich and Witholt, Bernard},
biburl = {https://puma.ub.uni-stuttgart.de/bibtex/23e8a85cb3ea345a70754b02b9d021e67/steffenhelbich},
interhash = {297edd6bcb35d13f8a5258f21f00a2b1},
intrahash = {3e8a85cb3ea345a70754b02b9d021e67},
issn = {1462-2912},
journal = {Environmental microbiology},
keywords = {alr engesser iswa},
number = 11,
pages = {676--682},
timestamp = {2018-09-05T11:54:39.000+0200},
title = {Alkane hydroxylase homologues in Gram-positive strains},
volume = 4,
year = 2002
}