%0 Journal Article %1 ISI:000175804200006 %A Gerigk, MR %A Maass, D %A Kreutzer, A %A Sprenger, G %A Bongaerts, J %A Wubbolts, M %A Takors, R %C 233 SPRING ST, NEW YORK, NY 10013 USA %D 2002 %I SPRINGER %J BIOPROCESS AND BIOSYSTEMS ENGINEERING %K myown %N 1 %P 43-52 %R 10.1007/s00449-002-0280-2 %T Enhanced pilot-scale fed-batch L-phenylalanine production with recombinant Escherichia coli by fully integrated reactive extraction %U https://doi.org/10.1007/s00449-002-0280-2 %V 25 %X A fully integrated process for the microbial production and recovery of the aromatic amino acid L-phenylalanine is presented. Using a recombinant L-tyrosine (L-Tyr) auxotrophic Escherichia coli production strain, a fed-batch fermentation process was developed in a 20-1-scale bioreactor. Concentrations of glucose and L-Tyr were closed-loop-controlled in a fed-batch process. After achieving final L-phenylalanine (L-Phe) titres >30 g/l the process strategy was scaled up to 300-1 pilot scale. In technical scale fermentation L-phenylalanine was continuously recovered via a fully integrated reactive extraction system achieving a maximum extraction rate of 110 g/h (final purity >99\%). It was thus possible to increase L-Phe/glucose selectivity from 15 mol\% without to 20.3 mol\% with integrated product separation.

@article{ISI:000175804200006, abstract = {{A fully integrated process for the microbial production and recovery of the aromatic amino acid L-phenylalanine is presented. Using a recombinant L-tyrosine (L-Tyr) auxotrophic Escherichia coli production strain, a fed-batch fermentation process was developed in a 20-1-scale bioreactor. Concentrations of glucose and L-Tyr were closed-loop-controlled in a fed-batch process. After achieving final L-phenylalanine (L-Phe) titres >30 g/l the process strategy was scaled up to 300-1 pilot scale. In technical scale fermentation L-phenylalanine was continuously recovered via a fully integrated reactive extraction system achieving a maximum extraction rate of 110 g/h (final purity >99\%). It was thus possible to increase L-Phe/glucose selectivity from 15 mol\% without to 20.3 mol\% with integrated product separation.}}, added-at = {2018-06-08T11:34:05.000+0200}, address = {{233 SPRING ST, NEW YORK, NY 10013 USA}}, affiliation = {{Takors, R (Reprint Author), Forschungszentrum Julich, Inst Biotechnol 2, D-52425 Julich, Germany. Forschungszentrum Julich, Inst Biotechnol 2, D-52425 Julich, Germany. Forschungszentrum Julich, Inst Biotechnol 1, D-52425 Julich, Germany. DSM Biotech GmbH, D-52428 Julich, Germany.}}, author = {Gerigk, MR and Maass, D and Kreutzer, A and Sprenger, G and Bongaerts, J and Wubbolts, M and Takors, R}, author-email = {{r.takors@fz-juelich.de}}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/21ceab909f64a601a0e9c2eab14d09010/ralftakors}, da = {{2018-01-26}}, doc-delivery-number = {{555QF}}, doi = {{10.1007/s00449-002-0280-2}}, interhash = {4b60bfbff6c62c981c9afc1c4a8d1ff5}, intrahash = {1ceab909f64a601a0e9c2eab14d09010}, issn = {{1615-7591}}, journal = {{BIOPROCESS AND BIOSYSTEMS ENGINEERING}}, journal-iso = {{Bioprocess. Biosyst. Eng.}}, keywords = {myown}, keywords-plus = {{LACTIC-ACID PRODUCTION; CORYNEBACTERIUM-GLUTAMICUM; FERMENTATION BROTH; EXPRESSION VECTOR; FEEDING STRATEGY; GROWING-CELLS; CITRIC-ACID; D-GLUCOSE; STRAIN; PHENYLPYRUVATE}}, language = {{English}}, month = {{APR}}, number = {{1}}, number-of-cited-references = {{79}}, orcid-numbers = {{Sprenger, Georg/0000-0002-7879-8978}}, pages = {{43-52}}, publisher = {{SPRINGER}}, research-areas = {{Biotechnology \& Applied Microbiology; Engineering}}, researcherid-numbers = {{Sprenger, Georg/E-2384-2011}}, times-cited = {{33}}, timestamp = {2018-06-08T09:34:05.000+0200}, title = {{Enhanced pilot-scale fed-batch L-phenylalanine production with recombinant Escherichia coli by fully integrated reactive extraction}}, type = {{Article}}, unique-id = {{ISI:000175804200006}}, url = {https://doi.org/10.1007/s00449-002-0280-2}, usage-count-last-180-days = {{2}}, usage-count-since-2013 = {{14}}, volume = {{25}}, web-of-science-categories = {{Biotechnology \& Applied Microbiology; Engineering, Chemical}}, year = {{2002}} }