%0 Journal Article %1 Faude2007 %A Faude, Alexander %A Zacher, Dörthe %A Müller, Egbert %A Böttinger, Heiner %D 2007 %J Journal of Chromatography A %K 2007 boettinger izi %N 1-2 %P 29--35 %R 10.1016/j.chroma.2007.03.114 %T Fast determination of conditions for maximum dynamic capacity in cation-exchange chromatography of human monoclonal antibodies %U https://www.ncbi.nlm.nih.gov/pubmed/17442329 %V 1161 %X Dynamic binding capacity (DBC) measurements of cation-exchange resins were performed with two human monoclonal antibodies. DBC showed a pH dependent maximum, which was shifted to lower pH values with increasing buffer concentrations and increasing salting-out effect of the buffer anion according to the Hofmeister series. As this downshift correlates well with zeta potential values, a measurement of the latter allows the determination of the pH value for maximum DBC under a given set of conditions. Thus, the use of zeta potential values can accelerate the purification process development and helps to understand the protein adsorption mechanism. © 2007 Elsevier B.V. All rights reserved. %Z Faude, Alexander Zacher, Dorthe Muller, Egbert Bottinger, Heiner eng Research Support, Non-U.S. Gov't Netherlands J Chromatogr A. 2007 Aug 17;1161(1-2):29-35. doi: 10.1016/j.chroma.2007.03.114. Epub 2007 Apr 4. %7 2007/04/20 %@ 0021-9673 (Print)$\backslash$r0021-9673 (Linking)

@article{Faude2007, abstract = {Dynamic binding capacity (DBC) measurements of cation-exchange resins were performed with two human monoclonal antibodies. DBC showed a pH dependent maximum, which was shifted to lower pH values with increasing buffer concentrations and increasing salting-out effect of the buffer anion according to the Hofmeister series. As this downshift correlates well with zeta potential values, a measurement of the latter allows the determination of the pH value for maximum DBC under a given set of conditions. Thus, the use of zeta potential values can accelerate the purification process development and helps to understand the protein adsorption mechanism. {\textcopyright} 2007 Elsevier B.V. All rights reserved.}, added-at = {2023-06-29T13:07:55.000+0200}, annote = {Faude, Alexander Zacher, Dorthe Muller, Egbert Bottinger, Heiner eng Research Support, Non-U.S. Gov't Netherlands J Chromatogr A. 2007 Aug 17;1161(1-2):29-35. doi: 10.1016/j.chroma.2007.03.114. Epub 2007 Apr 4.}, author = {Faude, Alexander and Zacher, D{\"{o}}rthe and M{\"{u}}ller, Egbert and B{\"{o}}ttinger, Heiner}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/2081f6dc3e29e076983ea0100cd0d1335/fabian}, doi = {10.1016/j.chroma.2007.03.114}, edition = {2007/04/20}, interhash = {6832b5bb0df2d3252b481791acf04eed}, intrahash = {081f6dc3e29e076983ea0100cd0d1335}, isbn = {0021-9673 (Print)$\backslash$r0021-9673 (Linking)}, issn = {00219673}, journal = {Journal of Chromatography A}, keywords = {2007 boettinger izi}, number = {1-2}, pages = {29--35}, pmid = {17442329}, timestamp = {2023-06-29T13:07:55.000+0200}, title = {{Fast determination of conditions for maximum dynamic capacity in cation-exchange chromatography of human monoclonal antibodies}}, url = {https://www.ncbi.nlm.nih.gov/pubmed/17442329}, volume = 1161, year = 2007 }