%0 Journal Article %1 ISI:A1993LC03000008 %A SIEMANN, M %A SYLDATK, C %A WAGNER, F %C 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8HN %D 1993 %I CHAPMAN HALL LTD %J BIOTECHNOLOGY TECHNIQUES %K imported myown %N 5 %P 361-366 %R 10.1007/BF00152542 %T DETECTION AND COMPARISON OF STRAINS WITH SELECTIVE L-HYDANTOIN CLEAVING ACTIVITY USING POLYCLONAL ANTIBODIES %U https://doi.org/10.1007/BF00152542 %V 7 %X Polyclonal antibodies were produced against the highly purified enzymes L-hydantoinase, hydantoin-racemase and L-N-carbamoylamino acid amidohydrolase of Arthrobacter aurescens DSM 3747. Using these antibodies as screening tools, a colony transfer procedure allowed the rapid detection of highly active strains. Furthermore, common Western-blot analysis enabled the direct estimation of internal enzyme concentrations, protein turnover, efficiency of different inducers and a molecular comparison of enzymes derived from different sources. Using this method it is demonstrated that of several tested microorganisms, two Arthrobacter strains showed improved production abilities.

@article{ISI:A1993LC03000008, abstract = {{Polyclonal antibodies were produced against the highly purified enzymes L-hydantoinase, hydantoin-racemase and L-N-carbamoylamino acid amidohydrolase of Arthrobacter aurescens DSM 3747. Using these antibodies as screening tools, a colony transfer procedure allowed the rapid detection of highly active strains. Furthermore, common Western-blot analysis enabled the direct estimation of internal enzyme concentrations, protein turnover, efficiency of different inducers and a molecular comparison of enzymes derived from different sources. Using this method it is demonstrated that of several tested microorganisms, two Arthrobacter strains showed improved production abilities.}}, added-at = {2018-01-25T13:38:08.000+0100}, address = {{2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8HN}}, affiliation = {{SIEMANN, M (Reprint Author), TECH UNIV CAROLO WILHELMINA BRAUNSCHWEIG,INST BIOCHEM \& BIOTECHNOL,KONSTANTIN UHDE ST 5,W-3300 BRAUNSCHWEIG,GERMANY.}}, author = {SIEMANN, M and SYLDATK, C and WAGNER, F}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/2f148bcd11ef7a562ae62de0aa13ff91b/siemannherzberg}, da = {{2018-01-25}}, doc-delivery-number = {{LC030}}, doi = {{10.1007/BF00152542}}, interhash = {f67fd21208d2c452190b651f7a9f3698}, intrahash = {f148bcd11ef7a562ae62de0aa13ff91b}, issn = {{0951-208X}}, journal = {{BIOTECHNOLOGY TECHNIQUES}}, journal-iso = {{Biotechnol. Tech.}}, keywords = {imported myown}, language = {{English}}, month = {{MAY}}, number = {{5}}, number-of-cited-references = {{11}}, pages = {{361-366}}, publisher = {{CHAPMAN HALL LTD}}, research-areas = {{Biochemistry \& Molecular Biology; Biotechnology \& Applied Microbiology}}, times-cited = {{7}}, timestamp = {2018-01-25T12:38:18.000+0100}, title = {{DETECTION AND COMPARISON OF STRAINS WITH SELECTIVE L-HYDANTOIN CLEAVING ACTIVITY USING POLYCLONAL ANTIBODIES}}, type = {{Article}}, unique-id = {{ISI:A1993LC03000008}}, url = {https://doi.org/10.1007/BF00152542}, usage-count-last-180-days = {{0}}, usage-count-since-2013 = {{2}}, volume = {{7}}, web-of-science-categories = {{Biochemical Research Methods; Biotechnology \& Applied Microbiology}}, year = {{1993}} }