PUMA publications for /group/simtech/materialTue Mar 10 11:32:12 CET 2026Journal of Applied Physiology03Shaping Smooth Muscle Forces: The Role of Preconditioning in Urinary Smooth Muscle2026bladder material adaptation biological urinary contraction tissue free soft properties Smooth muscle (SM) exhibits rapid mechanical adaptation in response to various stimuli, posing challenges for reproducible experimental results and consistent material parameter determination in biomechanical modeling. Preconditioning involving repeated loading and unloading cycles are commonly used to stabilize mechanical responses prior to testing. However, their influence on tissue properties and data variability remains underexplored. This study compares the effects of three preconditioning routines – passive cycling (PCYC), no preconditioning (PNPC), and free contraction (PFC) – on the active and passive force responses of porcine urinary bladder (UB) SM tissue. Three tissue strips from 12 UBs were randomly assigned to one of the routines and underwent an identical protocol involving a passive stretch ramp and two isometric contractions (IC1, IC2) to evaluate active and passive force development. After PCYC, the tissue generated the highest active (IC2: 44.7 ± 29.4 kPa) and passive tensions (IC2: 5.6 ± 4.3 kPa), though it also showed the highest variance in active tension. PNPC resulted in the lowest variance in active tension with a coefficient of variation (CV) of 45%, and PFC showed the lowest variance in passive tension, CV = 57%. These findings imply that the decision for a certain preconditioning protocol influences the observed mechanical properties. In this context, PFC appears promising for minimizing passive force variability and preventing creep-induced lengthening. This could offer a more reliable foundation for subsequent experiments analyzing mechanical parameters. This study underscores the importance of customized preconditioning strategies to enhance consistency and comparability in SM research and organ modeling.Fri Jul 20 10:54:15 CEST 2018Enabling Manufacturing Competitiveness and Economic Sustainability316-321System Dynamic Models and Real-time Simulation of Complex Material Flow Systems2012simulation; Real-time models system; Material dynamic vorlaeufig flow System