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         "type" : "Publication",
         "id"   : "https://puma.ub.uni-stuttgart.de/bibtex/29ce34124eaffe2a799aade38deec413d/bastian",         
         "tags" : [
            "Bacterial","Bioreactors,","Butylene","Complex,","Corynebacterium","Dehydrogenase","Dehydrogenase,","Engineering","Family,","Glucose,","Glycols,","L-Lactate","Lactococcus","Metabolic","Multigene","Oxygen,","Proteins,","Pyruvate","glutamicum,","lactis,","myown"
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         "intraHash" : "9ce34124eaffe2a799aade38deec413d",
         "interHash" : "518ec5750d920964df1a659788edff11",
         "label" : "Engineering Corynebacterium glutamicum for the production of 2,3-butanediol",
         "user" : "bastian",
         "description" : "",
         "date" : "2018-02-09 13:18:17",
         "changeDate" : "2018-02-09 12:18:56",
         "count" : 1,
         "pub-type": "article",
         "journal": "Microb. Cell Fact.",
         "year": "2015", 
         "url": "", 
         
         "author": [ 
            "Dušica Radoš","Ana Lúcia Carvalho","Stefan Wieschalka","Ana Rute Neves","Bastian Blombach","Bernhard J. Eikmanns","Helena Santos"
         ],
         "authors": [
         	
            	{"first" : "Dušica",	"last" : "Radoš"},
            	{"first" : "Ana Lúcia",	"last" : "Carvalho"},
            	{"first" : "Stefan",	"last" : "Wieschalka"},
            	{"first" : "Ana Rute",	"last" : "Neves"},
            	{"first" : "Bastian",	"last" : "Blombach"},
            	{"first" : "Bernhard J.",	"last" : "Eikmanns"},
            	{"first" : "Helena",	"last" : "Santos"}
         ],
         "volume": "14","pages": "171","abstract": "BACKGROUND: 2,3-Butanediol is an important bulk chemical with a wide range of applications. In bacteria, this metabolite is synthesised from pyruvate via a three-step pathway involving α-acetolactate synthase, α-acetolactate decarboxylase and 2,3-butanediol dehydrogenase. Thus far, the best producers of 2,3-butanediol are pathogenic strains, hence, the development of more suitable organisms for industrial scale fermentation is needed. Herein, 2,3-butanediol production was engineered in the Generally Regarded As Safe (GRAS) organism Corynebacterium glutamicum. A two-stage fermentation process was implemented: first, cells were grown aerobically on acetate; in the subsequent production stage cells were used to convert glucose into 2,3-butanediol under non-growing and oxygen-limiting conditions.\nRESULTS: A gene cluster, encoding the 2,3-butanediol biosynthetic pathway of Lactococcus lactis, was assembled and expressed in background strains, C. glutamicum ΔldhA, C. glutamicum ΔaceEΔpqoΔldhA and C. glutamicum ΔaceEΔpqoΔldhAΔmdh, tailored to minimize pyruvate-consuming reactions, i.e., to prevent carbon loss in lactic, acetic and succinic acids. Producer strains were characterized in terms of activity of the relevant enzymes in the 2,3-butanediol forming pathway, growth, and production of 2,3-butanediol under oxygen-limited conditions. Productivity was maximized by manipulating the aeration rate in the production phase. The final strain, C. glutamicum ΔaceEΔpqoΔldhAΔmdh(pEKEx2-als,aldB,Ptuf butA), under optimized conditions produced 2,3-butanediol with a 0.66 mol mol(-1) yield on glucose, an overall productivity of 0.2 g L(-1) h(-1) and a titer of 6.3 g L(-1).\nCONCLUSIONS: We have successfully developed C. glutamicum into an efficient cell factory for 2,3-butanediol production. The use of the engineered strains as a basis for production of acetoin, a widespread food flavour, is proposed.",
         "pmid" : "26511723",
         
         "issn" : "1475-2859",
         
         "pmcid" : "PMC4625470",
         
         "language" : "eng",
         
         "doi" : "10.1186/s12934-015-0362-x",
         
         "bibtexKey": "rados_engineering_2015"

      }
	  
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}