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         "type" : "Publication",
         "id"   : "https://puma.ub.uni-stuttgart.de/bibtex/2bc1670b16ed84beabd8820d13e46b2d2/bastian",         
         "tags" : [
            "Acids,","Bacterial,","Corynebacterium","Deletion,","Engineering,","Expression,","Gene","Genes,","Genetic","Genetically","Glucose,","Keto","Metabolic","Modified","Networks","Organisms,","Pathways,","and","glutamicum,","myown"
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         "intraHash" : "bc1670b16ed84beabd8820d13e46b2d2",
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         "label" : "Metabolic engineering of Corynebacterium glutamicum for 2-ketoisovalerate production",
         "user" : "bastian",
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         "date" : "2018-02-09 13:18:17",
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         "pub-type": "article",
         "journal": "Appl. Environ. Microbiol.",
         "year": "2010", 
         "url": "", 
         
         "author": [ 
            "Felix S. Krause","Bastian Blombach","Bernhard J. Eikmanns"
         ],
         "authors": [
         	
            	{"first" : "Felix S.",	"last" : "Krause"},
            	{"first" : "Bastian",	"last" : "Blombach"},
            	{"first" : "Bernhard J.",	"last" : "Eikmanns"}
         ],
         "volume": "76","number": "24","pages": "8053--8061","abstract": "2-Ketoisovalerate is used as a therapeutic agent, and a 2-ketoisovalerate-producing organism may serve as a platform for products deriving from this 2-keto acid. We engineered the wild type of Corynebacterium glutamicum for the growth-decoupled production of 2-ketoisovalerate from glucose by deletion of the aceE gene encoding the E1p subunit of the pyruvate dehydrogenase complex, deletion of the transaminase B gene ilvE, and additional overexpression of the ilvBNCD genes, encoding the l-valine biosynthetic enzymes acetohydroxyacid synthase (AHAS), acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. 2-Ketoisovalerate production was further improved by deletion of the pyruvate:quinone oxidoreductase gene pqo. In fed-batch fermentations at high cell densities, the newly constructed strains produced up to 188 ± 28 mM (21.8 ± 3.2 g liter(-1)) 2-ketoisovalerate and showed a product yield of about 0.47 ± 0.05 mol per mol (0.3 ± 0.03 g per g) of glucose and a volumetric productivity of about 4.6 ± 0.6 mM (0.53 ± 0.07 g liter(-1)) 2-ketoisovalerate per h in the overall production phase. In studying the influence of the three branched-chain 2-keto acids 2-ketoisovalerate, 2-ketoisocaproate, and 2-keto-3-methylvalerate on the AHAS activity, we observed a competitive inhibition of the AHAS enzyme by 2-ketoisovalerate.",
         "pmid" : "20935122",
         
         "issn" : "1098-5336",
         
         "pmcid" : "PMC3008247",
         
         "language" : "eng",
         
         "doi" : "10.1128/AEM.01710-10",
         
         "bibtexKey": "krause_metabolic_2010"

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