{ "types" : { "Bookmark" : { "pluralLabel" : "Bookmarks" }, "Publication" : { "pluralLabel" : "Publications" }, "GoldStandardPublication" : { "pluralLabel" : "GoldStandardPublications" }, "GoldStandardBookmark" : { "pluralLabel" : "GoldStandardBookmarks" }, "Tag" : { "pluralLabel" : "Tags" }, "User" : { "pluralLabel" : "Users" }, "Group" : { "pluralLabel" : "Groups" }, "Sphere" : { "pluralLabel" : "Spheres" } }, "properties" : { "count" : { "valueType" : "number" }, "date" : { "valueType" : "date" }, "changeDate" : { "valueType" : "date" }, "url" : { "valueType" : "url" }, "id" : { "valueType" : "url" }, "tags" : { "valueType" : "item" }, "user" : { "valueType" : "item" } }, "items" : [ { "type" : "Publication", "id" : "https://puma.ub.uni-stuttgart.de/bibtex/295af4cf87ffa1c7f150a05deb8bb810d/inspo5", "tags" : [ "bladder","material","adaptation","biological","urinary","contraction","tissue","free","soft","properties" ], "intraHash" : "95af4cf87ffa1c7f150a05deb8bb810d", "interHash" : "ad5f2b6a3128f8eb0f36ab2826d465bb", "label" : "Shaping Smooth Muscle Forces: The Role of Preconditioning in Urinary Smooth Muscle", "user" : "inspo5", "description" : "", "date" : "2026-03-10 11:32:12", "changeDate" : "2026-03-10 11:32:12", "count" : 1, "pub-type": "article", "journal": "Journal of Applied Physiology","publisher":"American Physiological Society", "year": "2026", "url": "http://dx.doi.org/10.1152/japplphysiol.00782.2025", "author": [ "Simon Kiem","Stefan Papenkort","Mischa Borsdorf","Markus Böl","Tobias Siebert" ], "authors": [ {"first" : "Simon", "last" : "Kiem"}, {"first" : "Stefan", "last" : "Papenkort"}, {"first" : "Mischa", "last" : "Borsdorf"}, {"first" : "Markus", "last" : "Böl"}, {"first" : "Tobias", "last" : "Siebert"} ], "editor": [ "Tobias Siebert" ], "editors": [ {"first" : "Tobias", "last" : "Siebert"} ], "abstract": "Smooth muscle (SM) exhibits rapid mechanical adaptation in response to various stimuli, posing challenges for reproducible experimental results and consistent material parameter determination in biomechanical modeling. Preconditioning involving repeated loading and unloading cycles are commonly used to stabilize mechanical responses prior to testing. However, their influence on tissue properties and data variability remains underexplored. This study compares the effects of three preconditioning routines \u2013 passive cycling (PCYC), no preconditioning (PNPC), and free contraction (PFC) \u2013 on the active and passive force responses of porcine urinary bladder (UB) SM tissue. Three tissue strips from 12 UBs were randomly assigned to one of the routines and underwent an identical protocol involving a passive stretch ramp and two isometric contractions (IC1, IC2) to evaluate active and passive force development. After PCYC, the tissue generated the highest active (IC2: 44.7 ± 29.4 kPa) and passive tensions (IC2: 5.6 ± 4.3 kPa), though it also showed the highest variance in active tension. PNPC resulted in the lowest variance in active tension with a coefficient of variation (CV) of 45%, and PFC showed the lowest variance in passive tension, CV = 57%. These findings imply that the decision for a certain preconditioning protocol influences the observed mechanical properties. In this context, PFC appears promising for minimizing passive force variability and preventing creep-induced lengthening. This could offer a more reliable foundation for subsequent experiments analyzing mechanical parameters. This study underscores the importance of customized preconditioning strategies to enhance consistency and comparability in SM research and organ modeling.", "language" : "English", "issn" : "1522-1601", "preprinturl" : "https://journals.physiology.org/doi/abs/10.1152/japplphysiol.00782.2025", "doi" : "10.1152/japplphysiol.00782.2025", "bibtexKey": "Kiem_2026" } , { "type" : "Publication", "id" : "https://puma.ub.uni-stuttgart.de/bibtex/2e278e56571667204e41214ae82b33ff8/inspo5", "tags" : [ "microstructure","Soleus","experiments","Plantaris","tissue","characteristics","cuniculus","muscles","Morphology","Mechanical","muscle","Oryctolagus","Muscle","dependent","Gastrocnemius","Age" ], "intraHash" : "e278e56571667204e41214ae82b33ff8", "interHash" : "69776921c88e79d221d10d5313558951", "label" : "Age-dependent properties of the rabbit calf musculature \u2014 Relationship between mechanic and microstructure", "user" : "inspo5", "description" : "", "date" : "2025-05-15 15:36:41", "changeDate" : "2025-05-15 15:36:41", "count" : 2, "pub-type": "article", "journal": "Journal of the Mechanical Behavior of Biomedical Materials","publisher":"Elsevier BV", "year": "2025", "url": "http://dx.doi.org/10.1016/j.jmbbm.2025.107008", "author": [ "Markus Böl","Kay Leichsenring","Steven Kutschke","Fabian Walter","Tobias Siebert" ], "authors": [ {"first" : "Markus", "last" : "Böl"}, {"first" : "Kay", "last" : "Leichsenring"}, {"first" : "Steven", "last" : "Kutschke"}, {"first" : "Fabian", "last" : "Walter"}, {"first" : "Tobias", "last" : "Siebert"} ], "editor": [ "Tobias Siebert" ], "editors": [ {"first" : "Tobias", "last" : "Siebert"} ], "volume": "168","pages": "107008","abstract": "In order to meet the requirements of body weight and height and the associated changing tasks and movement patterns during the growth of living bodies, significant changes in the skeletal musculature occur during this phase. In this study, the age-dependent (between 21 and 100 days) mechanical and microstructural tissue behaviour of the calf musculature, consisting of soleus muscles (SOL), gastrocnemius muscles (GAS) and plantaris muscles (PLA), was examined. To this end, cubic muscle tissue samples were examined using axial and semi-confined compression experiments. In addition, the essential muscle tissue components (muscle fibres, extracellular matrix, remaining components) were analysed. In a final step, these results were linked to morphological properties of the animals and muscles (animal mass, muscle mass, tibia length). Interestingly, the mechanical properties of the individual muscle types hardly differ from each other during growth, while both the morphological and microstructural properties change significantly. Thus, a clear increase of all morphological parameters (animal mass by 850%, muscle mass by 1000% (SOL), 1183% (GAS) and 1050% (PLA), tibia length by 235%) can be seen. In comparison, the microstructural parameters show a less consistent trend. The proportion of muscle fibres in the tissue cross-section increases by about 138% in the SOL, whereas the fibre proportion in both the GAS and PLA increases by only 109%. Consequently, the ECM proportion in the tissue cross-section decreases by 48%, 58% and 52% for SOL, GAS and PLA. Overall, the data obtained her e provides a deeper understanding of muscle growth and, in particular, of different muscle types that have different functions inside the calf. On the other hand, these data represent a good and comprehensive basis for later model developments.", "language" : "English", "issn" : "1751-6161", "doi" : "10.1016/j.jmbbm.2025.107008", "bibtexKey": "B_l_2025" } , { "type" : "Publication", "id" : "https://puma.ub.uni-stuttgart.de/bibtex/22f25a1adb4eeebb5ccf477386453f217/inspo5", "tags" : [ "Urinary","bladder","Stimulation","Stress\u2013strain-relationship","Biological","tissue","soft","Adaptation" ], "intraHash" : "2f25a1adb4eeebb5ccf477386453f217", "interHash" : "0aba6dffcddeb953c4db46121e64f033", "label" : "Reproducibility of smooth muscle mechanical properties in consecutive stretch and activation protocols", "user" : "inspo5", "description" : "", "date" : "2025-03-24 14:33:50", "changeDate" : "2025-03-24 14:33:50", "count" : 1, "pub-type": "article", "journal": "Pfluegers Archiv - European Journal of Physiology", "year": "2025", "url": "https://doi.org/10.1007/s00424-025-03075-7", "author": [ "Simon Kiem","Stefan Papenkort","Mischa Borsdorf","Markus Böl","Tobias Siebert" ], "authors": [ {"first" : "Simon", "last" : "Kiem"}, {"first" : "Stefan", "last" : "Papenkort"}, {"first" : "Mischa", "last" : "Borsdorf"}, {"first" : "Markus", "last" : "Böl"}, {"first" : "Tobias", "last" : "Siebert"} ], "editor": [ "Tobias Siebert" ], "editors": [ {"first" : "Tobias", "last" : "Siebert"} ], "abstract": "Mechanical organ models are crucial for understanding organ function and clinical applications. These models rely on input data regarding smooth muscle properties, typically gathered from experiments involving stimulations at different muscle lengths. However, reproducibility of these experimental results is a major challenge due to rapid changes in active and passive smooth muscle properties during the measurement period. Usually, preconditioning of the tissue is employed to ensure reproducible behavior in subsequent experiments, but this process itself alters the tissue's mechanical properties. To address this issue, three protocols (P1, P2, P3) without preconditioning were developed and compared to preserve the initial mechanical properties of smooth muscle tissue. Each protocol included five repetitive experimental cycles with stimulations at a long muscle length, varying in the number of stimulations at a short muscle length (P1: 0, P2: 1, P3: 2 stimulations). Results showed that P2 and P3 successfully reproduced the initial active force at a long length over five cycles, but failed to maintain the initial passive forces. Conversely, P1 was most effective in maintaining constant passive forces over the cycles. These findings are supported by existing adaptation models. Active force changes are primarily due to the addition or removal of contractile units in the contractile apparatus, while passive force changes mainly result from actin polymerization induced by contractions, leading to cytoskeletal stiffening. This study introduces a new method for obtaining reproducible smooth muscle parameters, offering a foundation for future research to replicate the mechanical properties of smooth muscle tissue without preconditioning.", "language" : "English", "issn" : "1432-2013", "doi" : "10.1007/s00424-025-03075-7", "bibtexKey": "Kiem2025" } , { "type" : "Publication", "id" : "https://puma.ub.uni-stuttgart.de/bibtex/2deb9c7133f0e87a8a038d2eef271ff4d/inspo5", "tags" : [ "stressporcinestress-stretch","dependenceactivation","curvestretch","tissue","dynamicsviscoelasticity","mechanicsmechanical","stiffnessoptimum" ], "intraHash" : "deb9c7133f0e87a8a038d2eef271ff4d", "interHash" : "f326d60d77a791ddce4e2d6c2c3dd148", "label" : "Active and passive material response of urinary bladder smooth muscle tissue in uniaxial and biaxial tensile testing", "user" : "inspo5", "description" : "", "date" : "2024-12-19 15:49:36", "changeDate" : "2024-12-19 15:49:36", "count" : 2, "pub-type": "article", "journal": "Acta Biomaterialia", "year": " 2024", "url": "https://www.sciencedirect.com/journal/acta-biomaterialia", "author": [ "Julian Geldner","Stefan Papenkort","Simon Kiem","Markus Böl","Tobias Siebert" ], "authors": [ {"first" : "Julian", "last" : "Geldner"}, {"first" : "Stefan", "last" : "Papenkort"}, {"first" : "Simon", "last" : "Kiem"}, {"first" : "Markus", "last" : "Böl"}, {"first" : "Tobias", "last" : "Siebert"} ], "editor": [ "Tobias Siebert" ], "editors": [ {"first" : "Tobias", "last" : "Siebert"} ], "volume": "18","abstract": "The urinary bladder is a hollow organ that undergoes significant deformation as it receives, stores, and releases urine. To understand the organ mechanics, it is necessary to obtain information about the material properties of the tissues involved. In displacement-controlled tensile tests, tissue samples are mounted on a device that applies stretches to the tissue in one or more directions, resulting in a specific stress response. For this study, we performed uniaxial and biaxial stretch experiments on tissue samples (n\u202F=\u202F36) from the body region of the porcine urinary bladder. We analyzed the stress-relaxation, activation dynamics, and passive and active stretch-stress response. Main findings of our experiments are: (1) For uniaxial and biaxial stretching, the time constants for stress-relaxation depend on the stretch amplitude, (2) biaxially stretched samples experienced slower activation with \r\n increasing by 163% compared to uniaxial stretching, (3) biaxial tests are characterized by reduced optimum stretches \r\n by -18%, and (4) biaxial and uniaxial tests showed no significant difference in maximum active stresses \r\n To interpret the results, we present a continuum mechanical model based on a viscoelastic, isotropic solid extended by a set of active muscle fibers. Model predictions show that results (3) and (4) can be explained by a uniform distribution of fiber orientations and a specific shape of the active fiber stress-stretch relationship. This study highlights how deformation modes during tensile testing affects smooth muscle mechanics, proving insights for interpreting experimental data and improving organ modeling.", "doi" : "10.1016/j.actbio.2024.12.045", "bibtexKey": "geldner2024active" } , { "type" : "Publication", "id" : "https://puma.ub.uni-stuttgart.de/bibtex/283fba02b010557af3f547c378cdb6de6/inspo5", "tags" : [ "fiber","Intramuscular","tissue","matrix","Extracellular","integrity","transmission","Endomysium","Connective","Muscle","force" ], "intraHash" : "83fba02b010557af3f547c378cdb6de6", "interHash" : "2d12b002242e8974ec406082ad65e8b3", "label" : "Endomysium determines active and passive force production in muscle fibers", "user" : "inspo5", "description" : "", "date" : "2024-05-08 11:25:57", "changeDate" : "2024-08-07 11:33:21", "count" : 8, "pub-type": "article", "journal": "Journal of Biomechanics","publisher":"Elsevier BV", "year": "2024", "url": "http://dx.doi.org/10.1016/j.jbiomech.2024.112134", "author": [ "Paolo Carlo Danesini","Maximilian Heim","André Tomalka","Tobias Siebert","Filiz Ates" ], "authors": [ {"first" : "Paolo", "last" : "Carlo Danesini"}, {"first" : "Maximilian", "last" : "Heim"}, {"first" : "André", "last" : "Tomalka"}, {"first" : "Tobias", "last" : "Siebert"}, {"first" : "Filiz", "last" : "Ates"} ], "editor": [ "Tobias Siebert" ], "editors": [ {"first" : "Tobias", "last" : "Siebert"} ], "volume": "168","pages": "112134","abstract": "Connective tissues can be recognized as an important structural support element in muscles. Recent studies have also highlighted its importance in active force generation and transmission between muscles, particularly through the epimysium. In the present study, we aimed to investigate the impact of the endomysium, the connective tissue surrounding muscle fibers, on both passive and active force production. Pairs of skeletal muscle fibers were extracted from the extensor digitorum longus muscles of rats and, after chemical skinning, their passive and active force\u2013length relationships were measured under two conditions: (i) with the endomysium between muscle fibers intact, and (ii) after its dissection. We found that the dissection of the endomysium caused force to significantly decrease in both active (by 22.2 % when normalized to the maximum isometric force; p < 0.001) and passive conditions (by 25.9 % when normalized to the maximum isometric force; p = 0.034). These findings indicate that the absence of endomysium compromises muscle fiber\u2019s not only passive but also active force production. This effect may be attributed to increased heterogeneity in sarcomere lengths, enhanced lattice spacing between myofilaments, or a diminished role of trans-sarcolemmal proteins due to dissecting the endomysium. Future investigations into the underlying mechanisms and their implications for various extracellular matrix-related diseases are warranted.", "language" : "English", "issn" : "0021-9290", "doi" : "10.1016/j.jbiomech.2024.112134", "bibtexKey": "Carlo_Danesini_2024" } , { "type" : "Publication", "id" : "https://puma.ub.uni-stuttgart.de/bibtex/2eeb7eec4365e95de248e73401073785a/inspo5", "tags" : [ "Biaxial","Biological","Stress-strain-relationship","mechanics","stomach","tissue","Inspo","testing","tensile","soft","Porcine","Organ" ], "intraHash" : "eeb7eec4365e95de248e73401073785a", "interHash" : "252446e0dc715c88dc9bb6f9988761ec", "label" : "Regional differences in stomach stretch during organ filling and their implications on the mechanical stress response", "user" : "inspo5", "description" : "", "date" : "2024-04-30 15:05:35", "changeDate" : "2024-04-30 15:05:35", "count" : 2, "pub-type": "article", "journal": "Journal of Biomechanics","publisher":"Elsevier BV", "year": "2024", "url": "http://dx.doi.org/10.1016/j.jbiomech.2024.112107", "author": [ "Stefan Papenkort","Mischa Borsdorf","Simon Kiem","Markus Böl","Tobias Siebert" ], "authors": [ {"first" : "Stefan", "last" : "Papenkort"}, {"first" : "Mischa", "last" : "Borsdorf"}, {"first" : "Simon", "last" : "Kiem"}, {"first" : "Markus", "last" : "Böl"}, {"first" : "Tobias", "last" : "Siebert"} ], "editor": [ "Tobias Siebert" ], "editors": [ {"first" : "Tobias", "last" : "Siebert"} ], "volume": "168","pages": "112107","abstract": "As part of the digestive system, the stomach plays a crucial role in the health and well-being of an organism. It produces acids and performs contractions that initiate the digestive process and begin the break-up of ingested food. Therefore, its mechanical properties are of interest. This study includes a detailed investigation of strains in the porcine stomach wall during passive organ filling. In addition, the observed strains were applied to tissue samples subjected to biaxial tensile tests. The results show inhomogeneous strains during filling, which tend to be higher in the circumferential direction (antrum: 13.2%, corpus: 22.0%, fundus: 67.8%), compared to the longitudinal direction (antrum: 4.8%, corpus: 24.7%, fundus: 50.0%) at a maximum filling of 3500 ml. Consequently, the fundus region experienced the greatest strain. In the biaxial tensile experiments, the corpus region appeared to be the stiffest, reaching nominal stress values above 400 kPa in the circumferential direction, whereas the other regions only reached stress levels of below 50 kPa in both directions for the investigated stretch range. Our findings gain new insight into stomach mechanics and provide valuable data for the development and validation of computational stomach models.", "language" : "English", "issn" : "0021-9290", "doi" : "10.1016/j.jbiomech.2024.112107", "bibtexKey": "Papenkort_2024" } , { "type" : "Publication", "id" : "https://puma.ub.uni-stuttgart.de/bibtex/243c7d8d7611bdcf9c01955ccd18e1576/inspo5", "tags" : [ "tension","Biaxial","experiments","staining","Layer","specific","Tissue","muscle","testing","Smooth","Microstructure" ], "intraHash" : "43c7d8d7611bdcf9c01955ccd18e1576", "interHash" : "1c551a3d7362260e9dc45aeb1d271fa4", "label" : "Location- and layer-dependent biomechanical and microstructural characterisation of the porcine urinary bladder wall", "user" : "inspo5", "description" : "", "date" : "2022-07-19 11:10:29", "changeDate" : "2022-07-19 09:10:56", "count" : 2, "pub-type": "article", "journal": "Journal of the Mechanical Behavior of Biomedical Materials","publisher":"Elsevier BV", "year": "2021", "url": "https://doi.org/10.1016%2Fj.jmbbm.2020.104275", "author": [ "Robin Trostorf","Enrique Morales-Orcajo","Tobias Siebert","Markus Böl" ], "authors": [ {"first" : "Robin", "last" : "Trostorf"}, {"first" : "Enrique", "last" : "Morales-Orcajo"}, {"first" : "Tobias", "last" : "Siebert"}, {"first" : "Markus", "last" : "Böl"} ], "editor": [ "Tobias Siebert" ], "editors": [ {"first" : "Tobias", "last" : "Siebert"} ], "volume": "115","pages": "104275", "doi" : "10.1016/j.jmbbm.2020.104275", "bibtexKey": "Trostorf_2021" } , { "type" : "Publication", "id" : "https://puma.ub.uni-stuttgart.de/bibtex/2c5478835f2cd88ec490ace0e656a5b8f/inspo5", "tags" : [ "gastric","relation","tissue","enhancement","force-velocity","contraction-behavior","muscle","motility","depression","force","smooth","force-length","properties" ], "intraHash" : "c5478835f2cd88ec490ace0e656a5b8f", "interHash" : "a5eeda529640c3525644d1772eb0cba6", "label" : "Porcine Stomach Smooth Muscle Force Depends on History-Effects", "user" : "inspo5", "description" : "", "date" : "2022-07-19 11:10:29", "changeDate" : "2022-07-19 09:14:49", "count" : 2, "pub-type": "article", "journal": "Front Physiol", "year": "2017", "url": "https://www.ncbi.nlm.nih.gov/pubmed/29093684", "author": [ "A. Tomalka","M. Borsdorf","M. Böl","T. Siebert" ], "authors": [ {"first" : "A.", "last" : "Tomalka"}, {"first" : "M.", "last" : "Borsdorf"}, {"first" : "M.", "last" : "Böl"}, {"first" : "T.", "last" : "Siebert"} ], "volume": "8","pages": "802","abstract": "The stomach serves as food reservoir, mixing organ and absorption area for certain substances, while continually varying its position and size. Large dimensional changes during ingestion and gastric emptying of the stomach are associated with large changes in smooth muscle length. These length changes might induce history-effects, namely force depression (FD) following active muscle shortening and force enhancement (FE) following active muscle stretch. Both effects have impact on the force generating capacity of the stomach, and thus functional relevance. However, less is known about history-effects and active smooth muscle properties of stomach smooth muscle. Thus, the aim of this study was to investigate biomechanical muscle properties as force-length and force-velocity relations (FVR) of porcine stomach smooth muscle strips, extended by the analysis of history-effects on smooth muscle force. Therefore, in total n = 54 tissue strips were dissected in longitudinal direction from the ventral fundus of porcine stomachs. Different isometric, isotonic, and isokinetic contraction protocols were performed during electrical muscle stimulation. Cross-sectional areas (CSA) of smooth muscles were determined from cryo-histological sections stained with Picrosirius Red. Results revealed that maximum smooth muscle tension was 10.4 ± 2.6 N/cm2. Maximum shortening velocity (Vmax ) and curvature factor (curv) of the FVR were 0.04 ± 0.01 [optimum muscle length/s] and 0.36 ± 0.15, respectively. The findings of the present study demonstrated significant (P < 0.05) FD [up to 32% maximum muscle force (Fim )] and FE (up to 16% Fim ) of gastric muscle tissue, respectively. The FE- and FD-values increased with increasing ramp amplitude. This outstanding muscle behavior is not accounted for in existing models so far and strongly supports the idea of a holistic reflection of distinct stomach structure and function. For the first time this study provides a comprehensive set of stomach smooth muscle parameters including classic biomechanical muscle properties and history-dependent effects, offering the possibility for the development and validation of computational stomach models. Furthermore, this data set facilitates novel insights in gastric motility and contraction behavior based on the re-evaluation of existing contractile mechanisms. That will likely help to understand physiological functions or dysfunctions in terms of gastric accommodation and emptying.", "issn" : "1664-042X (Print)\r\n1664-042X (Linking)", "doi" : "10.3389/fphys.2017.00802", "bibtexKey": "RN6295" } , { "type" : "Publication", "id" : "https://puma.ub.uni-stuttgart.de/bibtex/20e9dbe076502380e2b8e70a80da95818/inspo5", "tags" : [ "Semi-confined","Soleus","tissue","characteristics","cuniculus","Microstructural","Axial","tests","Age-dependent","Inspo","muscle","Oryctolagus","Morphometry","Siebert","compression","properties" ], "intraHash" : "0e9dbe076502380e2b8e70a80da95818", "interHash" : "6afbb85807dbc73f3599b68ac7ec0018", "label" : "Age-dependent mechanical and microstructural properties of the rabbit soleus muscle", "user" : "inspo5", "description" : "", "date" : "2022-07-19 11:10:29", "changeDate" : "2022-07-19 09:10:56", "count" : 1, "pub-type": "article", "journal": "Acta Biomaterialia","publisher":"Elsevier BV", "year": "2021", "url": "https://doi.org/10.1016%2Fj.actbio.2021.07.066", "author": [ "Kay Leichsenring","Asha Viswanathan","Steven Kutschke","Markus Siebert, TobiasBöl" ], "authors": [ {"first" : "Kay", "last" : "Leichsenring"}, {"first" : "Asha", "last" : "Viswanathan"}, {"first" : "Steven", "last" : "Kutschke"}, {"first" : "Markus", "last" : "Siebert, TobiasBöl"} ], "editor": [ "Tobias Siebert" ], "editors": [ {"first" : "Tobias", "last" : "Siebert"} ], "volume": "134","pages": "453--465","abstract": "During growth there are serious changes in the skeletal muscles to compensate for the changed requirements in terms of body weight and size. In this study, the age-dependent (between 21 and 100 days) mechanical and microstructural properties of rabbit soleus muscle tissue were investigated. For this purpose, morphological properties (animal mass, soleus muscle mass, tibial length) were measured at 5 different times during aging. On the other hand, fibre orientation-dependent axial and semi-confined compression experiments were realised. In addition, the essential components (muscle fibres, extracellular matrix, remaining components), dominating the microstructure of muscle tissue, were analysed. While the mechanical results show hardly any age-dependent differences, the morphological and microstructural results show clear age-dependent differences. All morphological parameters increase significantly (animal mass by 839.2%, muscle mass 1050.6%, tibial length 233.6%). In contrast, microstructural parameters change differently. The percentage of fibres (divided into slow-twitch (ST) and fast-twitch (FT) fibres) increases significantly (137.6%), while the proportions of the extracellular matrix and the remaining components (48.2% and 46.1%) decrease. At the same time, the cross-sectional area of the fibres increases significantly (697.9%). The totality of this age-dependent information provides a deeper understanding of age-related changes in muscle structure and function and may contribute to successful development and validation of growth models in the future.\n\nStatement of significance\nThis article reports the first comprehensive data set on age-dependent morphological (animal mass, soleus muscle mass, tibial length), mechanical (axial and semi-confined compression), and microstructural (muscle fibres, extracellular matrix, remaining components) properties of the rabbit soleus muscle. On the one hand, the results of this study contribute to the understanding of muscle mechanics and thus to understanding of load transfer mechanisms inside the muscle tissue during growth. On the other hand, these results are relevant to the fields of constitutive formulation of age-dependent muscle tissue.", "doi" : "10.1016/j.actbio.2021.07.066", "bibtexKey": "Leichsenring_2021" } , { "type" : "Publication", "id" : "https://puma.ub.uni-stuttgart.de/bibtex/2346fb7cde84b5e8f820d71aa9d8e0ce0/inspo5", "tags" : [ "Stomach","tension","Biaxial","staining","Tissue","composition","muscle","testingTissue","Smooth","Microstructure","wall" ], "intraHash" : "346fb7cde84b5e8f820d71aa9d8e0ce0", "interHash" : "2f5fd279259cd2399f3e049e26399fc7", "label" : "Biomechanical and microstructural characterisation of the porcine stomach wall: Location- and layer-dependent investigations", "user" : "inspo5", "description" : "", "date" : "2022-07-19 11:10:29", "changeDate" : "2022-07-19 09:10:56", "count" : 2, "pub-type": "article", "journal": "Acta Biomaterialia","publisher":"Elsevier BV", "year": "2020", "url": "https://doi.org/10.1016%2Fj.actbio.2019.11.038", "author": [ "Melanie Bauer","Enrique Morales-Orcajo","Lisa Klemm","Robert Seydewitz","Victoria Fiebach","Tobias Siebert","Markus Böl" ], "authors": [ {"first" : "Melanie", "last" : "Bauer"}, {"first" : "Enrique", "last" : "Morales-Orcajo"}, {"first" : "Lisa", "last" : "Klemm"}, {"first" : "Robert", "last" : "Seydewitz"}, {"first" : "Victoria", "last" : "Fiebach"}, {"first" : "Tobias", "last" : "Siebert"}, {"first" : "Markus", "last" : "Böl"} ], "editor": [ "Tobias Siebert" ], "editors": [ {"first" : "Tobias", "last" : "Siebert"} ], "volume": "102","pages": "83--99", "doi" : "10.1016/j.actbio.2019.11.038", "bibtexKey": "Bauer_2020" } ] }