{"a8704533a8dfa10f1d5f42ed6d4aef19slegewie":{"DOI":"10.1038/s41467-018-07455-9","ISBN":"","ISSN":"2041-1723","URL":"","abstract":"Even though proteins are produced from mRNA, the correlation between mRNA levels and protein abundances is moderate in most studies, occasionally attributed to complex post-transcriptional regulation. To address this, we generate a paired transcriptome/proteome time course dataset with 14 time points during Drosophila embryogenesis. Despite a limited mRNA-protein correlation (ρ = 0.54), mathematical models describing protein translation and degradation explain 84\\% of protein time-courses based on the measured mRNA dynamics without assuming complex post transcriptional regulation, and allow for classification of most proteins into four distinct regulatory scenarios. By performing an in-depth characterization of the putatively post-transcriptionally regulated genes, we postulate that the RNA-binding protein Hrb98DE is involved in post-transcriptional control of sugar metabolism in early embryogenesis and partially validate this hypothesis using Hrb98DE knockdown. In summary, we present a systems biology framework for the identification of post-transcriptional gene regulation from large-scale, time-resolved transcriptome and proteome data.","annote":"","author":[{"family":"Becker","given":"Kolja"},{"family":"Bluhm","given":"Alina"},{"family":"Casas-Vila","given":"Nuria"},{"family":"Dinges","given":"Nadja"},{"family":"Dejung","given":"Mario"},{"family":"Sayols","given":"Sergi"},{"family":"Kreutz","given":"Clemens"},{"family":"Roignant","given":"Jean-Yves"},{"family":"Butter","given":"Falk"},{"family":"Legewie","given":"Stefan"}],"citation-label":"becker_quantifying_2018","collection-editor":[],"collection-title":"","container-author":[],"container-title":"Nature Communications","documents":[],"edition":"","editor":[],"event-date":{"date-parts":[["2018","nov"]],"literal":"2018"},"event-place":"","id":"a8704533a8dfa10f1d5f42ed6d4aef19slegewie","interhash":"42325feb1689d483604c5222faf8ebb8","intrahash":"a8704533a8dfa10f1d5f42ed6d4aef19","issue":"1","issued":{"date-parts":[["2018","nov"]],"literal":"2018"},"keyword":"Animals, Base Development, Drosophila Embryonic Expression Gene Genetic, Glucose, Kinetics, Messenger, Proteins, Proteome, RNA, Regulation, Sequence, Transcription, Transcriptome melanogaster,","misc":{"language":"eng","pmid":"30478415","file":"Volltext:C\\:\\\\Users\\\\SL\\\\Zotero\\\\storage\\\\U4MU6BPV\\\\Becker et al. - 2018 - Quantifying post-transcriptional regulation in the development of Drosophila melanogaster.pdf:application/pdf","issn":"2041-1723","pmcid":"PMC6255845","doi":"10.1038/s41467-018-07455-9"},"note":"","number":"1","page":"4970","page-first":"4970","publisher":"","publisher-place":"","status":"","title":"Quantifying post-transcriptional regulation in the development of Drosophila melanogaster","type":"article-journal","username":"slegewie","version":"","volume":"9"}}