{"f3d78410850201349b3420cb0f71115ainspo5":{"DOI":"doi:10.1007/s00424-021-02568-5","ISBN":"","ISSN":"","URL":"https://link.springer.com/article/10.1007/s00424-021-02568-5","abstract":"Uniaxial tensile experiments are a standard method to determine the contractile properties of smooth muscles. Smooth muscle strips from organs of the urogenital and gastrointestinal tract contain multiple muscle layers with different muscle fiber orientations, which are frequently not separated for the experiments. During strip activation, these muscle fibers contract in deviant orientations from the force-measuring axis, affecting the biomechanical characteristics of the tissue strips. This study aimed to investigate the influence of muscle layer separation on the determination of smooth muscle properties. Smooth muscle strips, consisting of longitudinal and circumferential muscle layers (whole-muscle strips [WMS]), and smooth muscle strips, consisting of only the circumferential muscle layer (separated layer strips [SLS]), have been prepared from the fundus of the porcine stomach. Strips were mounted with muscle fibers of the circumferential layer inline with the force-measuring axis of the uniaxial testing setup. The force–length (FLR) and force–velocity relationships (FVR) were determined through a series of isometric and isotonic contractions, respectively. Muscle layer separation revealed no changes in the FLR. However, the SLS exhibited a higher maximal shortening velocity and a lower curvature factor than WMS. During WMS activation, the transversally oriented muscle fibers of the longitudinal layer shortened, resulting in a narrowing of this layer. Expecting volume constancy of muscle tissue, this narrowing leads to a lengthening of the longitudinal layer, which counteracted the shortening of the circumferential layer during isotonic contractions. Consequently, the shortening velocities of the WMS were decreased significantly. This effect was stronger at high shortening velocities.","annote":"","author":[{"family":"Borsdorf","given":"Mischa"},{"family":"Böl","given":"Markus"},{"family":"Siebert","given":"Tobias"}],"citation-label":"borsdorf2021influence","collection-editor":[{"family":"Siebert","given":"Tobias"}],"collection-title":"","container-author":[{"family":"Siebert","given":"Tobias"}],"container-title":"Pflugers Arch","documents":[],"edition":"","editor":[{"family":"Siebert","given":"Tobias"}],"event-date":{"date-parts":[["2021","06"]],"literal":"2021"},"event-place":"","id":"f3d78410850201349b3420cb0f71115ainspo5","interhash":"b79cfd3a81cd8360cba0a6d4729f7452","intrahash":"f3d78410850201349b3420cb0f71115a","issue":"","issued":{"date-parts":[["2021","06"]],"literal":"2021"},"keyword":"Stomach Uniaxial Force experiments velocity length layer relationship muscle Contractile Separated tensile wall Organ properties","misc":{"language":"English","doi":"doi:10.1007/s00424-021-02568-5"},"note":"","number":"","number-of-pages":"9","page":"911-920","page-first":"911","publisher":"","publisher-place":"","status":"","title":"Influence of layer separation on the determination of stomach smooth muscle properties.","type":"article-journal","username":"inspo5","version":"","volume":"473"},"43c7d8d7611bdcf9c01955ccd18e1576inspo5":{"DOI":"10.1016/j.jmbbm.2020.104275","ISBN":"","ISSN":"","URL":"https://doi.org/10.1016%2Fj.jmbbm.2020.104275","abstract":"","annote":"","author":[{"family":"Trostorf","given":"Robin"},{"family":"Morales-Orcajo","given":"Enrique"},{"family":"Siebert","given":"Tobias"},{"family":"Böl","given":"Markus"}],"citation-label":"Trostorf_2021","collection-editor":[{"family":"Siebert","given":"Tobias"}],"collection-title":"","container-author":[{"family":"Siebert","given":"Tobias"}],"container-title":"Journal of the Mechanical Behavior of Biomedical Materials","documents":[],"edition":"","editor":[{"family":"Siebert","given":"Tobias"}],"event-date":{"date-parts":[["2021","03"]],"literal":"2021"},"event-place":"","id":"43c7d8d7611bdcf9c01955ccd18e1576inspo5","interhash":"1c551a3d7362260e9dc45aeb1d271fa4","intrahash":"43c7d8d7611bdcf9c01955ccd18e1576","issue":"","issued":{"date-parts":[["2021","03"]],"literal":"2021"},"keyword":"tension Biaxial experiments staining Layer specific Tissue muscle testing Smooth Microstructure","misc":{"doi":"10.1016/j.jmbbm.2020.104275"},"note":"","number":"","page":"104275","page-first":"104275","publisher":"Elsevier BV","publisher-place":"","status":"","title":"Location- and layer-dependent biomechanical and microstructural characterisation of the porcine urinary bladder wall","type":"article-journal","username":"inspo5","version":"","volume":"115"},"769be1510640e0e78e217c26c4dffbb5mhartmann":{"DOI":"10.1007/s.11118-012-9310-0","ISBN":"","ISSN":"0926-2601","URL":"http://dx.doi.org/10.1007/s11118-012-9310-0","abstract":"","annote":"","author":[{"family":"Kohr","given":"Mirela"},{"family":"Lanza de Cristoforis","given":"Massimo"},{"family":"Wendland","given":"Wolfgang L."}],"citation-label":"kohr2013nonlinear","collection-editor":[],"collection-title":"","container-author":[],"container-title":"Potential Analysis","documents":[],"edition":"","editor":[],"event-date":{"date-parts":[["2013"]],"literal":"2013"},"event-place":"","id":"769be1510640e0e78e217c26c4dffbb5mhartmann","interhash":"d6d8c49bb72357b5a9ac7fe494eb266e","intrahash":"769be1510640e0e78e217c26c4dffbb5","issue":"","issued":{"date-parts":[["2013"]],"literal":"2013"},"keyword":"Nonlinear domain, Brinkman boundary problem, Layer 76M Lipschitz 42B20, Stokes 46E35, 35J25, and value vorlaeufig potential operators, 76D,","misc":{"issn":"0926-2601","language":"English","doi":"10.1007/s.11118-012-9310-0"},"note":"","number":"","number-of-pages":"48","page":"1123-1171","page-first":"1123","publisher":"","publisher-place":"","status":"","title":"Nonlinear Neumann-Transmission Problems for Stokes and Brinkman Equations\n\ton Euclidean Lipschitz Domains","type":"article-journal","username":"mhartmann","version":"","volume":"38"},"dc38376bf7860c4fe03db35f36425dd6mhartmann":{"DOI":"10.1002/zamm.201100194","ISBN":"","ISSN":"1521-4001","URL":"http://dx.doi.org/10.1002/zamm.201100194","abstract":"In this paper we use a layer potential analysis to show the existence\n\tof solutions for a Dirichlet-transmission problem for pseudodifferential\n\tBrinkman operators on Sobolev and Besov spaces associated to Lipschitz\n\tdomains in compact boundaryless Riemannian manifolds. Compactness\n\tand invertibility properties of corresponding layer potential operators\n\ton Lp, Sobolev, or Besov scales are also obtained.","annote":"","author":[{"family":"Kohr","given":"M."},{"family":"Pintea","given":"C."},{"family":"Wendland","given":"Wolfgang L."}],"citation-label":"kohr2013dirichlettransmission","collection-editor":[],"collection-title":"","container-author":[],"container-title":"ZAMM - Journal of Applied Mathematics and Mechanics / Zeitschrift\n\tfür Angewandte Mathematik und Mechanik","documents":[],"edition":"","editor":[],"event-date":{"date-parts":[["2013"]],"literal":"2013"},"event-place":"","id":"dc38376bf7860c4fe03db35f36425dd6mhartmann","interhash":"4b459931efee9dae6b660e7e30dbf7a3","intrahash":"dc38376bf7860c4fe03db35f36425dd6","issue":"","issued":{"date-parts":[["2013"]],"literal":"2013"},"keyword":"Brinkman compact Besov problem, Dirichlet-transmission domains, Riemannian Lipschitz layer spaces. and manifolds, Pseudodifferential Sobolev vorlaeufig potential operators,","misc":{"issn":"1521-4001","doi":"10.1002/zamm.201100194"},"note":"","number":"","number-of-pages":"12","page":"446-458","page-first":"446","publisher":"","publisher-place":"","status":"","title":"Dirichlet-transmission problems for pseudodifferential Brinkman operators\n\ton Sobolev and Besov spaces associated to Lipschitz domains in Riemannian\n\tmanifolds","type":"article-journal","username":"mhartmann","version":"","volume":"93"}}