PUMA publications for /user/siemannherzberg/stresshttps://puma.ub.uni-stuttgart.de/user/siemannherzberg/stressPUMA RSS feed for /user/siemannherzberg/stress2024-03-30T04:36:58+01:00Applying systems biology tools to study n-butanol degradation in
Pseudomonas putida KT2440https://puma.ub.uni-stuttgart.de/bibtex/2652fb4ff4b5092a491db6399dff0fe9d/siemannherzbergsiemannherzberg2018-01-25T13:38:08+01:00Butanol; Chemostat Omics; Stress culture; myown response} {Biofuels; <span data-person-type="author" class="authorEditorList "><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Tobias Vallon" itemprop="url" href="/person/14b90fac7e2fb842634a098f46bc45a2b/author/0"><span itemprop="name">T. Vallon</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Oliver Simon" itemprop="url" href="/person/14b90fac7e2fb842634a098f46bc45a2b/author/1"><span itemprop="name">O. Simon</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Beate Rendgen-Heugle" itemprop="url" href="/person/14b90fac7e2fb842634a098f46bc45a2b/author/2"><span itemprop="name">B. Rendgen-Heugle</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Sabine Frana" itemprop="url" href="/person/14b90fac7e2fb842634a098f46bc45a2b/author/3"><span itemprop="name">S. Frana</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bjoern Mueckschel" itemprop="url" href="/person/14b90fac7e2fb842634a098f46bc45a2b/author/4"><span itemprop="name">B. Mueckschel</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Alexander Broicher" itemprop="url" href="/person/14b90fac7e2fb842634a098f46bc45a2b/author/5"><span itemprop="name">A. Broicher</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Martin Siemann-Herzberg" itemprop="url" href="/person/14b90fac7e2fb842634a098f46bc45a2b/author/6"><span itemprop="name">M. Siemann-Herzberg</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Jens Pfannenstiel" itemprop="url" href="/person/14b90fac7e2fb842634a098f46bc45a2b/author/7"><span itemprop="name">J. Pfannenstiel</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bernhard Hauer" itemprop="url" href="/person/14b90fac7e2fb842634a098f46bc45a2b/author/8"><span itemprop="name">B. Hauer</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Achim Huber" itemprop="url" href="/person/14b90fac7e2fb842634a098f46bc45a2b/author/9"><span itemprop="name">A. Huber</span></a></span></span> and 2 other author(s). </span><span class="additional-entrytype-information"><span itemtype="http://schema.org/PublicationIssue" itemscope="itemscope" itemprop="isPartOf"><em><span itemprop="journal">ENGINEERING IN LIFE SCIENCES</span>, </em> <em><span itemtype="http://schema.org/PublicationVolume" itemscope="itemscope" itemprop="isPartOf"><span itemprop="volumeNumber">15 </span></span>(<span itemprop="issueNumber">8</span>):
<span itemprop="pagination">760-771</span></em> </span>(<em><span>November 2015<meta content="November 2015" itemprop="datePublished"/></span></em>)</span>Thu Jan 25 13:38:08 CET 2018{111 RIVER ST, HOBOKEN 07030-5774, NJ USA}{ENGINEERING IN LIFE SCIENCES}{NOV}{8}{760-771}{Applying systems biology tools to study n-butanol degradation in
Pseudomonas putida KT2440}{Article}{15}{2015}Butanol; Chemostat Omics; Stress culture; myown response} {Biofuels; {To smoothen the process of n-butanol formation in Pseudomonas putida
KT2440, detailed knowledge of the impact of this organic solvent on cell
physiology and regulation is of outmost importance. Here, we conducted a
detailed systems biology study to elucidate cellular responses at the
metabolic, proteomic, and transcriptional level. Pseudomonas putida
KT2440 was cultivated in multiple chemostat fermentations using
n-butanol either as sole carbon source or together with glucose.
Pseudomonas putida KT2440 revealed maximum growth rates (mu) of 0.3
h(-1) with n-butanol as sole carbon source and of 0.4 h(-1) using equal
C-molar amounts of glucose and n-butanol. While C-mole specific
substrate consumption and biomass/substrate yields appeared equal at
these growth conditions, the cellular physiology was found to be
substantially different: adenylate energy charge levels of 0.85 were
found when n-butanol served as sole carbon source (similar to glucose as
sole carbon source), but were reduced to 0.4 when n-butanol was
coconsumed at stable growth conditions. Furthermore, characteristic
maintenance parameters changed with increasing n-butanol consumption.
C-13 flux analysis revealed that central metabolism was split into a
glucose-fueled Entner-Doudoroff/pentose-phosphate pathway and an
n-butanol-fueled tricarboxylic acid cycle when both substrates were
coconsumed. With the help of transcriptome and proteome analysis, the
degradation pathway of n-butanol could be unraveled, thus representing
an important basis for rendering P. putida KT2440 from an n-butanol
consumer to a producer in future metabolic engineering studies.}