PUMA publications for /user/bastian/biorefinery,https://puma.ub.uni-stuttgart.de/user/bastian/biorefinery,PUMA RSS feed for /user/bastian/biorefinery,2024-03-28T20:54:11+01:00Valorization of pyrolysis water: a biorefinery side stream, for 1,2-propanediol production with engineered Corynebacterium glutamicumhttps://puma.ub.uni-stuttgart.de/bibtex/27ae233f38758c480db193ed5e7bbf7f7/bastianbastian2018-02-09T13:18:17+01:00(propylene 1,2-propanediol Bioeconomy, Biorefinery, Corynebacterium Fast Growth-coupled Lignocellulose, Metabolic Pyrolysis biotransformation, engineering, glutamicum, glycol), myown pyrolysis, water <span data-person-type="author" class="authorEditorList "><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Julian Lange" itemprop="url" href="/person/1dc474d4c00bd416fa2ec00ea366a50da/author/0"><span itemprop="name">J. Lange</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Felix Müller" itemprop="url" href="/person/1dc474d4c00bd416fa2ec00ea366a50da/author/1"><span itemprop="name">F. Müller</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Kerstin Bernecker" itemprop="url" href="/person/1dc474d4c00bd416fa2ec00ea366a50da/author/2"><span itemprop="name">K. Bernecker</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Nicolaus Dahmen" itemprop="url" href="/person/1dc474d4c00bd416fa2ec00ea366a50da/author/3"><span itemprop="name">N. Dahmen</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Ralf Takors" itemprop="url" href="/person/1dc474d4c00bd416fa2ec00ea366a50da/author/4"><span itemprop="name">R. Takors</span></a></span>, </span> and <span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bastian Blombach" itemprop="url" href="/person/1dc474d4c00bd416fa2ec00ea366a50da/author/5"><span itemprop="name">B. Blombach</span></a></span></span>. </span><span class="additional-entrytype-information"><span itemtype="http://schema.org/PublicationIssue" itemscope="itemscope" itemprop="isPartOf"><em><span itemprop="journal">Biotechnol Biofuels</span>, </em> </span>(<em><span>2017<meta content="2017" itemprop="datePublished"/></span></em>)</span>Fri Feb 09 13:18:17 CET 2018Biotechnol Biofuels277Valorization of pyrolysis water: a biorefinery side stream, for 1,2-propanediol production with engineered {Corynebacterium} glutamicum102017(propylene 1,2-propanediol Bioeconomy, Biorefinery, Corynebacterium Fast Growth-coupled Lignocellulose, Metabolic Pyrolysis biotransformation, engineering, glutamicum, glycol), myown pyrolysis, water Background: A future bioeconomy relies on the efficient use of renewable resources for energy and material product supply. In this context, biorefineries have been developed and play a key role in converting lignocellulosic residues. Although a holistic use of the biomass feed is desired, side streams evoke in current biorefinery approaches. To ensure profitability, efficiency, and sustainability of the overall conversion process, a meaningful valorization of these materials is needed. Here, a so far unexploited side stream derived from fast pyrolysis of wheat straw-pyrolysis water-was used for production of 1,2-propanediol in microbial fermentation with engineered Corynebacterium glutamicum.
Results: A protocol for pretreatment of pyrolysis water was established and enabled growth on its major constituents, acetate and acetol, with rates up to 0.36 ± 0.04 h-1. To convert acetol to 1,2-propanediol, the plasmid pJULgldA expressing the glycerol dehydrogenase from Escherichia coli was introduced into C. glutamicum. 1,2-propanediol was formed in a growth-coupled biotransformation and production was further increased by construction of C. glutamicum Δpqo ΔaceE ΔldhA Δmdh pJULgldA. In a two-phase aerobic/microaerobic fed-batch process with pyrolysis water as substrate, this strain produced 18.3 ± 1.2 mM 1,2-propanediol with a yield of 0.96 ± 0.05 mol 1,2-propanediol per mol acetol and showed an overall volumetric productivity of 1.4 ± 0.1 mmol 1,2-propanediol L-1 h-1.
Conclusions: This study implements microbial fermentation into a biorefinery based on pyrolytic liquefaction of lignocellulosic biomass and accesses a novel value chain by valorizing the side stream pyrolysis water for 1,2-PDO production with engineered C. glutamicum. The established bioprocess operated at maximal product yield and accomplished the so far highest overall volumetric productivity for microbial 1,2-PDO production with an engineered producer strain. Besides, the results highlight the potential of microbial conversion of this biorefinery side stream to other valuable products.