PUMA publications for /user/siemannherzberg/stability;https://puma.ub.uni-stuttgart.de/user/siemannherzberg/stability;PUMA RSS feed for /user/siemannherzberg/stability;2024-03-28T20:21:28+01:00High-cell-density fermentation for production of L-N-carbamoylase using
an expression system based on the Escherichia coli rhaBAD promoterhttps://puma.ub.uni-stuttgart.de/bibtex/2bb5d870973239ffef4428a38b60ade30/siemannherzbergsiemannherzberg2018-01-25T13:38:08+01:00L-N-carbamoylase; expression; fermentation; high-cell-density inducible myown plasmid promoter} rhamnose stability; {L-rhamnose <span data-person-type="author" class="authorEditorList "><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="B Wilms" itemprop="url" href="/person/1ad9a2e10f05138389f3df5928ffeb234/author/0"><span itemprop="name">B. Wilms</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="A Hauck" itemprop="url" href="/person/1ad9a2e10f05138389f3df5928ffeb234/author/1"><span itemprop="name">A. Hauck</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="M Reuss" itemprop="url" href="/person/1ad9a2e10f05138389f3df5928ffeb234/author/2"><span itemprop="name">M. Reuss</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="C Syldatk" itemprop="url" href="/person/1ad9a2e10f05138389f3df5928ffeb234/author/3"><span itemprop="name">C. Syldatk</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="R Mattes" itemprop="url" href="/person/1ad9a2e10f05138389f3df5928ffeb234/author/4"><span itemprop="name">R. Mattes</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="M Siemann" itemprop="url" href="/person/1ad9a2e10f05138389f3df5928ffeb234/author/5"><span itemprop="name">M. Siemann</span></a></span>, </span> and <span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="J Altenbuchner" itemprop="url" href="/person/1ad9a2e10f05138389f3df5928ffeb234/author/6"><span itemprop="name">J. Altenbuchner</span></a></span></span>. </span><span class="additional-entrytype-information"><span itemtype="http://schema.org/PublicationIssue" itemscope="itemscope" itemprop="isPartOf"><em><span itemprop="journal">BIOTECHNOLOGY AND BIOENGINEERING</span>, </em> <em><span itemtype="http://schema.org/PublicationVolume" itemscope="itemscope" itemprop="isPartOf"><span itemprop="volumeNumber">73 </span></span>(<span itemprop="issueNumber">2</span>):
<span itemprop="pagination">95-103</span></em> </span>(<em><span>April 2001<meta content="April 2001" itemprop="datePublished"/></span></em>)</span>Thu Jan 25 13:38:08 CET 2018{111 RIVER ST, HOBOKEN 07030-5774, NJ USA}{BIOTECHNOLOGY AND BIOENGINEERING}{APR 20}{2}{95-103}{High-cell-density fermentation for production of L-N-carbamoylase using
an expression system based on the Escherichia coli rhaBAD promoter}{Article}{73}{2001}L-N-carbamoylase; expression; fermentation; high-cell-density inducible myown plasmid promoter} rhamnose stability; {L-rhamnose {A high-cell-density fed-batch fermentation for the production of
heterologous proteins in Escherichia coli was developed using the
positively regulated Escherichia coli rhaBAD promoter. The expression
system was improved by reducing of the amount of expensive L-rhamnose
necessary for induction of the rhamnose promoter and by increasing the
vector stability. Consumption of the inducer L-rhamnose was inhibited by
inactivation of L-rhamnulose kinase encoding gene rhaB of Escherichia
coli W3110, responsible for the first irreversible step in rhamnose
catabolism. Plasmid instability caused by multimerization of the
expression vector in the recombination-proficient W3110 was prevented by
insertion of the multimer resolution site cer from the ColE1 plasmid
into the vector. Fermentation experiments with the optimized system
resulted in the production of 100 g L-1 cell dry weight and 3.8 g L-1 of
recombinant L-N-carbamoylase, an enzyme, which is needed for the
production of enantiomeric pure amino acids in a two-step reaction from
hydantoins, (C) 2001 John Wiley \& Sons, Inc.}