PUMA publications for /user/bastian/networks%20myown%20glutamicum,%20metabolomehttps://puma.ub.uni-stuttgart.de/user/bastian/networks%20myown%20glutamicum,%20metabolomePUMA RSS feed for /user/bastian/networks%20myown%20glutamicum,%20metabolome2024-03-29T07:38:13+01:00Importance of NADPH supply for improved L-valine formation in Corynebacterium glutamicumhttps://puma.ub.uni-stuttgart.de/bibtex/2dfb0ffb355b1bb961e53e70b66a327fd/bastianbastian2018-02-09T13:18:17+01:00Biological, Cell Corynebacterium Culture Fermentation, Glucose-6-Phosphate Isomerase, Media, Metabolic Metabolome Models, NADP, Networks Pathways, Techniques, Valine, and glutamicum, myown <span data-person-type="author" class="authorEditorList "><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Tobias Bartek" itemprop="url" href="/person/1ea026818f74ee7c252525689e9c0c32c/author/0"><span itemprop="name">T. Bartek</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bastian Blombach" itemprop="url" href="/person/1ea026818f74ee7c252525689e9c0c32c/author/1"><span itemprop="name">B. Blombach</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Enrico Zönnchen" itemprop="url" href="/person/1ea026818f74ee7c252525689e9c0c32c/author/2"><span itemprop="name">E. Zönnchen</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Pia Makus" itemprop="url" href="/person/1ea026818f74ee7c252525689e9c0c32c/author/3"><span itemprop="name">P. Makus</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Siegmund Lang" itemprop="url" href="/person/1ea026818f74ee7c252525689e9c0c32c/author/4"><span itemprop="name">S. Lang</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bernhard J. Eikmanns" itemprop="url" href="/person/1ea026818f74ee7c252525689e9c0c32c/author/5"><span itemprop="name">B. Eikmanns</span></a></span>, </span> and <span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Marco Oldiges" itemprop="url" href="/person/1ea026818f74ee7c252525689e9c0c32c/author/6"><span itemprop="name">M. Oldiges</span></a></span></span>. </span><span class="additional-entrytype-information"><span itemtype="http://schema.org/PublicationIssue" itemscope="itemscope" itemprop="isPartOf"><em><span itemprop="journal">Biotechnol. Prog.</span>, </em> <em><span itemtype="http://schema.org/PublicationVolume" itemscope="itemscope" itemprop="isPartOf"><span itemprop="volumeNumber">26 </span></span>(<span itemprop="issueNumber">2</span>):
<span itemprop="pagination">361--371</span></em> </span>(<em><span>April 2010<meta content="April 2010" itemprop="datePublished"/></span></em>)</span>Fri Feb 09 13:18:17 CET 2018Biotechnol. Prog.apr2361--371Importance of {NADPH} supply for improved {L}-valine formation in {Corynebacterium} glutamicum262010Biological, Cell Corynebacterium Culture Fermentation, Glucose-6-Phosphate Isomerase, Media, Metabolic Metabolome Models, NADP, Networks Pathways, Techniques, Valine, and glutamicum, myown Cofactor recycling is known to be crucial for amino acid synthesis. Hence, cofactor supply was now analyzed for L-valine to identify new targets for an improvement of production. The central carbon metabolism was analyzed by stoichiometric modeling to estimate the influence of cofactors and to quantify the theoretical yield of L-valine on glucose. Three different optimal routes for L-valine biosynthesis were identified by elementary mode (EM) analysis. The modes differed mainly in the manner of NADPH regeneration, substantiating that the cofactor supply may be crucial for efficient L-valine production. Although the isocitrate dehydrogenase as an NADPH source within the tricarboxylic acid cycle only enables an L-valine yield of Y(Val/Glc) = 0.5 mol L-valine/mol glucose (mol Val/mol Glc), the pentose phosphate pathway seems to be the most promising NADPH source. Based on the theoretical calculation of EMs, the gene encoding phosphoglucoisomerase (PGI) was deleted to achieve this EM with a theoretical yield Y(Val/Glc) = 0.86 mol Val/mol Glc during the production phase. The intracellular NADPH concentration was significantly increased in the PGI-deficient mutant. L-Valine yield increased from 0.49 +/- 0.13 to 0.67 +/- 0.03 mol Val/mol Glc, and, concomitantly, the formation of by-products such as pyruvate was reduced.