PUMA publications for /user/bastian/dehydrogenase%20complex,%20fermentation,%20myownhttps://puma.ub.uni-stuttgart.de/user/bastian/dehydrogenase%20complex,%20fermentation,%20myownPUMA RSS feed for /user/bastian/dehydrogenase%20complex,%20fermentation,%20myown2024-03-28T10:46:55+01:00L-valine production with pyruvate dehydrogenase complex-deficient Corynebacterium glutamicumhttps://puma.ub.uni-stuttgart.de/bibtex/25bac540150e10acba07b2f8205f6f070/bastianbastian2018-02-09T13:18:17+01:00myown Complex, Pyruvate Lysine, glutamicum, Pyruvic Acid, Alanine Valine, Dehydrogenase Corynebacterium Fermentation, Isoleucine, <span data-person-type="author" class="authorEditorList "><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bastian Blombach" itemprop="url" href="/person/1d744d92c5af8f45409137c58d022ef1c/author/0"><span itemprop="name">B. Blombach</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Mark E. Schreiner" itemprop="url" href="/person/1d744d92c5af8f45409137c58d022ef1c/author/1"><span itemprop="name">M. Schreiner</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Jirí Holátko" itemprop="url" href="/person/1d744d92c5af8f45409137c58d022ef1c/author/2"><span itemprop="name">J. Holátko</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Tobias Bartek" itemprop="url" href="/person/1d744d92c5af8f45409137c58d022ef1c/author/3"><span itemprop="name">T. Bartek</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Marco Oldiges" itemprop="url" href="/person/1d744d92c5af8f45409137c58d022ef1c/author/4"><span itemprop="name">M. Oldiges</span></a></span>, </span> and <span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bernhard J. Eikmanns" itemprop="url" href="/person/1d744d92c5af8f45409137c58d022ef1c/author/5"><span itemprop="name">B. Eikmanns</span></a></span></span>. </span><span class="additional-entrytype-information"><span itemtype="http://schema.org/PublicationIssue" itemscope="itemscope" itemprop="isPartOf"><em><span itemprop="journal">Appl. Environ. Microbiol.</span>, </em> <em><span itemtype="http://schema.org/PublicationVolume" itemscope="itemscope" itemprop="isPartOf"><span itemprop="volumeNumber">73 </span></span>(<span itemprop="issueNumber">7</span>):
<span itemprop="pagination">2079--2084</span></em> </span>(<em><span>April 2007<meta content="April 2007" itemprop="datePublished"/></span></em>)</span>Fri Feb 09 13:18:17 CET 2018Appl. Environ. Microbiol.0472079--2084L-valine production with pyruvate dehydrogenase complex-deficient {Corynebacterium} glutamicum732007myown Complex, Pyruvate Lysine, glutamicum, Pyruvic Acid, Alanine Valine, Dehydrogenase Corynebacterium Fermentation, Isoleucine, Corynebacterium glutamicum was engineered for the production of L-valine from glucose by deletion of the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. In the absence of cellular growth, C. glutamicum DeltaaceE showed a relatively high intracellular concentration of pyruvate (25.9 mM) and produced significant amounts of pyruvate, L-alanine, and L-valine from glucose as the sole carbon source. Lactate or acetate was not formed. Plasmid-bound overexpression of ilvBNCE in C. glutamicum DeltaaceE resulted in an approximately 10-fold-lower intracellular pyruvate concentration (2.3 mM) and a shift of the extracellular product pattern from pyruvate and L-alanine towards L-valine. In fed-batch fermentations at high cell densities and an excess of glucose, C. glutamicum DeltaaceE(pJC4ilvBNCE) produced up to 210 mM L-valine with a volumetric productivity of 10.0 mM h(-1) (1.17 g l(-1) h(-1)) and a maximum yield of about 0.6 mol per mol (0.4 g per g) of glucose.Effect of pyruvate dehydrogenase complex deficiency on L-lysine production with Corynebacterium glutamicumhttps://puma.ub.uni-stuttgart.de/bibtex/2930ecfe113b73f241ab0452b05337d89/bastianbastian2018-02-09T13:18:17+01:00Bacterial, myown Complex, Biotechnology Genes, Pyruvate Lysine, glutamicum, Dehydrogenase Deletion, Base Corynebacterium Kinetics, Expression, Fermentation, DNA, Gene Sequence, <span data-person-type="author" class="authorEditorList "><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bastian Blombach" itemprop="url" href="/person/197097cd65b8e231a2578efbc7586a5dc/author/0"><span itemprop="name">B. Blombach</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Mark E. Schreiner" itemprop="url" href="/person/197097cd65b8e231a2578efbc7586a5dc/author/1"><span itemprop="name">M. Schreiner</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Matthias Moch" itemprop="url" href="/person/197097cd65b8e231a2578efbc7586a5dc/author/2"><span itemprop="name">M. Moch</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Marco Oldiges" itemprop="url" href="/person/197097cd65b8e231a2578efbc7586a5dc/author/3"><span itemprop="name">M. Oldiges</span></a></span>, </span> and <span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bernhard J. Eikmanns" itemprop="url" href="/person/197097cd65b8e231a2578efbc7586a5dc/author/4"><span itemprop="name">B. Eikmanns</span></a></span></span>. </span><span class="additional-entrytype-information"><span itemtype="http://schema.org/PublicationIssue" itemscope="itemscope" itemprop="isPartOf"><em><span itemprop="journal">Appl. Microbiol. Biotechnol.</span>, </em> <em><span itemtype="http://schema.org/PublicationVolume" itemscope="itemscope" itemprop="isPartOf"><span itemprop="volumeNumber">76 </span></span>(<span itemprop="issueNumber">3</span>):
<span itemprop="pagination">615--623</span></em> </span>(<em><span>September 2007<meta content="September 2007" itemprop="datePublished"/></span></em>)</span>Fri Feb 09 13:18:17 CET 2018Appl. Microbiol. Biotechnol.093615--623Effect of pyruvate dehydrogenase complex deficiency on {L}-lysine production with {Corynebacterium} glutamicum762007Bacterial, myown Complex, Biotechnology Genes, Pyruvate Lysine, glutamicum, Dehydrogenase Deletion, Base Corynebacterium Kinetics, Expression, Fermentation, DNA, Gene Sequence, Intracellular precursor supply is a critical factor for amino acid productivity of Corynebacterium glutamicum. To test for the effect of improved pyruvate availability on L-lysine production, we deleted the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex (PDHC) in the L-lysine-producer C. glutamicum DM1729 and characterised the resulting strain DM1729-BB1 for growth and L-lysine production. Compared to the host strain, C. glutamicum DM1729-BB1 showed no PDHC activity, was acetate auxotrophic and, after complete consumption of the available carbon sources glucose and acetate, showed a more than 50\% lower substrate-specific biomass yield (0.14 vs 0.33 mol C/mol C), an about fourfold higher biomass-specific L-lysine yield (5.27 vs 1.23 mmol/g cell dry weight) and a more than 40\% higher substrate-specific L-lysine yield (0.13 vs 0.09 mol C/mol C). Overexpression of the pyruvate carboxylase or diaminopimelate dehydrogenase genes in C. glutamicum DM1729-BB1 resulted in a further increase in the biomass-specific L-lysine yield by 6 and 56\%, respectively. In addition to L-lysine, significant amounts of pyruvate, L-alanine and L-valine were produced by C. glutamicum DM1729-BB1 and its derivatives, suggesting a surplus of precursor availability and a further potential to improve L-lysine production by engineering the L-lysine biosynthetic pathway.