PUMA publications for /user/bastian/dehydrogenase%20complex,%20fermentation,%20myown%20isoleucine,https://puma.ub.uni-stuttgart.de/user/bastian/dehydrogenase%20complex,%20fermentation,%20myown%20isoleucine,PUMA RSS feed for /user/bastian/dehydrogenase%20complex,%20fermentation,%20myown%20isoleucine,2024-03-28T09:26:56+01:00L-valine production with pyruvate dehydrogenase complex-deficient Corynebacterium glutamicumhttps://puma.ub.uni-stuttgart.de/bibtex/25bac540150e10acba07b2f8205f6f070/bastianbastian2018-02-09T13:18:17+01:00Acid, Alanine Complex, Corynebacterium Dehydrogenase Fermentation, Isoleucine, Lysine, Pyruvate Pyruvic Valine, glutamicum, myown <span data-person-type="author" class="authorEditorList "><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bastian Blombach" itemprop="url" href="/person/1d744d92c5af8f45409137c58d022ef1c/author/0"><span itemprop="name">B. Blombach</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Mark E. Schreiner" itemprop="url" href="/person/1d744d92c5af8f45409137c58d022ef1c/author/1"><span itemprop="name">M. Schreiner</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Jirí Holátko" itemprop="url" href="/person/1d744d92c5af8f45409137c58d022ef1c/author/2"><span itemprop="name">J. Holátko</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Tobias Bartek" itemprop="url" href="/person/1d744d92c5af8f45409137c58d022ef1c/author/3"><span itemprop="name">T. Bartek</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Marco Oldiges" itemprop="url" href="/person/1d744d92c5af8f45409137c58d022ef1c/author/4"><span itemprop="name">M. Oldiges</span></a></span>, </span> and <span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bernhard J. Eikmanns" itemprop="url" href="/person/1d744d92c5af8f45409137c58d022ef1c/author/5"><span itemprop="name">B. Eikmanns</span></a></span></span>. </span><span class="additional-entrytype-information"><span itemtype="http://schema.org/PublicationIssue" itemscope="itemscope" itemprop="isPartOf"><em><span itemprop="journal">Appl. Environ. Microbiol.</span>, </em> <em><span itemtype="http://schema.org/PublicationVolume" itemscope="itemscope" itemprop="isPartOf"><span itemprop="volumeNumber">73 </span></span>(<span itemprop="issueNumber">7</span>):
<span itemprop="pagination">2079--2084</span></em> </span>(<em><span>April 2007<meta content="April 2007" itemprop="datePublished"/></span></em>)</span>Fri Feb 09 13:18:17 CET 2018Appl. Environ. Microbiol.apr72079--2084L-valine production with pyruvate dehydrogenase complex-deficient {Corynebacterium} glutamicum732007Acid, Alanine Complex, Corynebacterium Dehydrogenase Fermentation, Isoleucine, Lysine, Pyruvate Pyruvic Valine, glutamicum, myown Corynebacterium glutamicum was engineered for the production of L-valine from glucose by deletion of the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. In the absence of cellular growth, C. glutamicum DeltaaceE showed a relatively high intracellular concentration of pyruvate (25.9 mM) and produced significant amounts of pyruvate, L-alanine, and L-valine from glucose as the sole carbon source. Lactate or acetate was not formed. Plasmid-bound overexpression of ilvBNCE in C. glutamicum DeltaaceE resulted in an approximately 10-fold-lower intracellular pyruvate concentration (2.3 mM) and a shift of the extracellular product pattern from pyruvate and L-alanine towards L-valine. In fed-batch fermentations at high cell densities and an excess of glucose, C. glutamicum DeltaaceE(pJC4ilvBNCE) produced up to 210 mM L-valine with a volumetric productivity of 10.0 mM h(-1) (1.17 g l(-1) h(-1)) and a maximum yield of about 0.6 mol per mol (0.4 g per g) of glucose.