PUMA publications for /user/bastian/Organichttps://puma.ub.uni-stuttgart.de/user/bastian/OrganicPUMA RSS feed for /user/bastian/Organic2024-03-29T11:59:51+01:00The pyruvate dehydrogenase complex of Corynebacterium glutamicum: an attractive target for metabolic engineeringhttps://puma.ub.uni-stuttgart.de/bibtex/2ed56a8ffa25fcb7fe239b276b7e52493/bastianbastian2018-02-09T13:18:17+01:00myown Complex, Pyruvate glutamicum, complex Pyruvic Butanols, Acid, Metabolic Valine, Isobutanol Dehydrogenase dehydrogenase Corynebacterium acid production, Engineering, and engineering, Amino organic <span data-person-type="author" class="authorEditorList "><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bernhard J. Eikmanns" itemprop="url" href="/person/111bc42d6baae0b586acdbed655538eb6/author/0"><span itemprop="name">B. Eikmanns</span></a></span>, </span> and <span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bastian Blombach" itemprop="url" href="/person/111bc42d6baae0b586acdbed655538eb6/author/1"><span itemprop="name">B. Blombach</span></a></span></span>. </span><span class="additional-entrytype-information"><span itemtype="http://schema.org/PublicationIssue" itemscope="itemscope" itemprop="isPartOf"><em><span itemprop="journal">J. Biotechnol.</span>, </em> </span>(<em><span>December 2014<meta content="December 2014" itemprop="datePublished"/></span></em>)</span>Fri Feb 09 13:18:17 CET 2018J. Biotechnol.12339--345The pyruvate dehydrogenase complex of {Corynebacterium} glutamicum: an attractive target for metabolic engineering192 Pt B2014myown Complex, Pyruvate glutamicum, complex Pyruvic Butanols, Acid, Metabolic Valine, Isobutanol Dehydrogenase dehydrogenase Corynebacterium acid production, Engineering, and engineering, Amino organic The pyruvate dehydrogenase complex (PDHC) catalyzes the oxidative thiamine pyrophosphate-dependent decarboxylation of pyruvate to acetyl-CoA and CO2. Since pyruvate is a key metabolite of the central metabolism and also the precursor for several relevant biotechnological products, metabolic engineering of this multienzyme complex is a promising strategy to improve microbial production processes. This review summarizes the current knowledge and achievements on metabolic engineering approaches to tailor the PDHC of Corynebacterium glutamicum for the bio-based production of l-valine, 2-ketosiovalerate, pyruvate, succinate and isobutanol and to improve l-lysine production.Bio-based production of organic acids with Corynebacterium glutamicumhttps://puma.ub.uni-stuttgart.de/bibtex/2a7bae842f703e05319d0ca548f4461fb/bastianbastian2018-02-09T13:18:17+01:00myown Organic glutamicum, Genetic Biotechnology, Metabolic Carboxylic Engineering Corynebacterium Fermentation, Engineering, Acids, Chemicals, <span data-person-type="author" class="authorEditorList "><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Stefan Wieschalka" itemprop="url" href="/person/13585759eabf5f155131ea9827284ca79/author/0"><span itemprop="name">S. Wieschalka</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bastian Blombach" itemprop="url" href="/person/13585759eabf5f155131ea9827284ca79/author/1"><span itemprop="name">B. Blombach</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Michael Bott" itemprop="url" href="/person/13585759eabf5f155131ea9827284ca79/author/2"><span itemprop="name">M. Bott</span></a></span>, </span> and <span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bernhard J. Eikmanns" itemprop="url" href="/person/13585759eabf5f155131ea9827284ca79/author/3"><span itemprop="name">B. Eikmanns</span></a></span></span>. </span><span class="additional-entrytype-information"><span itemtype="http://schema.org/PublicationIssue" itemscope="itemscope" itemprop="isPartOf"><em><span itemprop="journal">Microb Biotechnol</span>, </em> <em><span itemtype="http://schema.org/PublicationVolume" itemscope="itemscope" itemprop="isPartOf"><span itemprop="volumeNumber">6 </span></span>(<span itemprop="issueNumber">2</span>):
<span itemprop="pagination">87--102</span></em> </span>(<em><span>March 2013<meta content="March 2013" itemprop="datePublished"/></span></em>)</span>Fri Feb 09 13:18:17 CET 2018Microb Biotechnol03287--102Bio-based production of organic acids with {Corynebacterium} glutamicum62013myown Organic glutamicum, Genetic Biotechnology, Metabolic Carboxylic Engineering Corynebacterium Fermentation, Engineering, Acids, Chemicals, The shortage of oil resources, the steadily rising oil prices and the impact of its use on the environment evokes an increasing political, industrial and technical interest for development of safe and efficient processes for the production of chemicals from renewable biomass. Thus, microbial fermentation of renewable feedstocks found its way in white biotechnology, complementing more and more traditional crude oil-based chemical processes. Rational strain design of appropriate microorganisms has become possible due to steadily increasing knowledge on metabolism and pathway regulation of industrially relevant organisms and, aside from process engineering and optimization, has an outstanding impact on improving the performance of such hosts. Corynebacterium glutamicum is well known as workhorse for the industrial production of numerous amino acids. However, recent studies also explored the usefulness of this organism for the production of several organic acids and great efforts have been made for improvement of the performance. This review summarizes the current knowledge and recent achievements on metabolic engineering approaches to tailor C. glutamicum for the bio-based production of organic acids. We focus here on the fermentative production of pyruvate, L- and D-lactate, 2-ketoisovalerate, 2-ketoglutarate, and succinate. These organic acids represent a class of compounds with manifold application ranges, e.g. in pharmaceutical and cosmetics industry, as food additives, and economically very interesting, as precursors for a variety of bulk chemicals and commercially important polymers.