PUMA publications for /user/bastian/Dissolved%20carbon%20alanine,%20regulation,%20biosynthesishttps://puma.ub.uni-stuttgart.de/user/bastian/Dissolved%20carbon%20alanine,%20regulation,%20biosynthesisPUMA RSS feed for /user/bastian/Dissolved%20carbon%20alanine,%20regulation,%20biosynthesis2024-03-29T12:35:01+01:00Impact of different CO2/HCO3- levels on metabolism and regulation in Corynebacterium glutamicumhttps://puma.ub.uni-stuttgart.de/bibtex/24eb5c8cfb1d770de7b680bff723465f7/bastianbastian2018-02-09T13:18:17+01:00Alanine, Bacterial Bacterial, Biomass, CO(2)/HCO(3)(−), Carbon Corynebacterium DNA-Binding Dehydrogenase, Dioxide, Diphtheria Dissolved DtxR, Expression Gene Glucose, Glucosephosphate Proteins, Regulation, Thiamin Thiamine, Toxin, Valine, biosynthesis carbon dioxide, glutamicum, myown repressor toxin <span data-person-type="author" class="authorEditorList "><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bastian Blombach" itemprop="url" href="/person/175535e85947aaa1c453c03c26dfd2dfc/author/0"><span itemprop="name">B. Blombach</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Jens Buchholz" itemprop="url" href="/person/175535e85947aaa1c453c03c26dfd2dfc/author/1"><span itemprop="name">J. Buchholz</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Tobias Busche" itemprop="url" href="/person/175535e85947aaa1c453c03c26dfd2dfc/author/2"><span itemprop="name">T. Busche</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Jörn Kalinowski" itemprop="url" href="/person/175535e85947aaa1c453c03c26dfd2dfc/author/3"><span itemprop="name">J. Kalinowski</span></a></span>, </span> und <span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Ralf Takors" itemprop="url" href="/person/175535e85947aaa1c453c03c26dfd2dfc/author/4"><span itemprop="name">R. Takors</span></a></span></span>. </span><span class="additional-entrytype-information"><span itemtype="http://schema.org/PublicationIssue" itemscope="itemscope" itemprop="isPartOf"><em><span itemprop="journal">J. Biotechnol.</span>, </em> <em><span itemtype="http://schema.org/PublicationVolume" itemscope="itemscope" itemprop="isPartOf"><span itemprop="volumeNumber">168 </span></span>(<span itemprop="issueNumber">4</span>):
<span itemprop="pagination">331--340</span></em> </span>(<em><span>Dezember 2013<meta content="Dezember 2013" itemprop="datePublished"/></span></em>)</span>Fri Feb 09 13:18:17 CET 2018J. Biotechnol.dec4331--340Impact of different {CO}2/{HCO}3- levels on metabolism and regulation in {Corynebacterium} glutamicum1682013Alanine, Bacterial Bacterial, Biomass, CO(2)/HCO(3)(−), Carbon Corynebacterium DNA-Binding Dehydrogenase, Dioxide, Diphtheria Dissolved DtxR, Expression Gene Glucose, Glucosephosphate Proteins, Regulation, Thiamin Thiamine, Toxin, Valine, biosynthesis carbon dioxide, glutamicum, myown repressor toxin We investigated the growth kinetics and transcriptional responses of Corynebacterium glutamicum in environments with low (pCO2{\textless}40 mbar) and high (pCO2 ≥ 300 mbar) CO2/HCO3(-) levels compared to standard conditions. When cultivated at high CO2/HCO3(-)-levels, C. glutamicum showed increased (63\%) biomass to substrate yields during the initial growth phase. Other kinetic parameters such as growth rate (μ), specific glucose consumption rate (qS), and selected enzymatic activities of anaplerotic reactions, the pentose phosphate pathway and the tricarboxylic acid cycle were similar to standard conditions. However, microarray hybridization disclosed a complex transcriptional response involving 117 differentially expressed genes. Among those, 60 genes were assigned to the complete DtxR/RipA regulon controlling iron homeostasis in C. glutamicum. Impaired growth of a ΔdtxR mutant at high CO2/HCO3(-) levels validated the relevance of this master regulator to cope with excessive CO2/HCO3(-) availability. At low CO2/HCO3(-) levels, C. glutamicum grew in a bi-level manner with three distinct growth phases. Differential analyses revealed approximately doubled activities of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase accompanied by the formation of L-alanine and L-valine during the lowest μ occurring in mid-phase of the cultivation. DNA microarray analysis revealed more than 100 differentially expressed genes in growth phase II compared to phase I including almost all thiamin pyrophosphate (TPP) biosynthesis genes, which were significantly up regulated. Concluding, we hypothesize that C. glutamicum counteracts the lack of CO2/HCO3(-) by triggering TPP biosynthesis for increasing the activities of TPP-dependent enzymes involved in CO2 formation.