PUMA publications for /user/bastian/Dehydrogenase%20gene%20corynebacterium%20valine,https://puma.ub.uni-stuttgart.de/user/bastian/Dehydrogenase%20gene%20corynebacterium%20valine,PUMA RSS feed for /user/bastian/Dehydrogenase%20gene%20corynebacterium%20valine,2024-03-29T15:28:55+01:00L-valine production during growth of pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum in the presence of ethanol or by inactivation of the transcriptional regulator SugRhttps://puma.ub.uni-stuttgart.de/bibtex/205af8a7b28d60ba8272685f0427eaadb/bastianbastian2018-02-09T13:18:17+01:00Complex Corynebacterium Dehydrogenase Ethanol, Factors, Gene Knockout Pyruvate Techniques, Transcription Valine, glutamicum, myown <span data-person-type="author" class="authorEditorList "><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bastian Blombach" itemprop="url" href="/person/1581b084966b1a19b10f06b5ed069aa58/author/0"><span itemprop="name">B. Blombach</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Annette Arndt" itemprop="url" href="/person/1581b084966b1a19b10f06b5ed069aa58/author/1"><span itemprop="name">A. Arndt</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Marc Auchter" itemprop="url" href="/person/1581b084966b1a19b10f06b5ed069aa58/author/2"><span itemprop="name">M. Auchter</span></a></span>, </span> and <span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bernhard J. Eikmanns" itemprop="url" href="/person/1581b084966b1a19b10f06b5ed069aa58/author/3"><span itemprop="name">B. Eikmanns</span></a></span></span>. </span><span class="additional-entrytype-information"><span itemtype="http://schema.org/PublicationIssue" itemscope="itemscope" itemprop="isPartOf"><em><span itemprop="journal">Appl. Environ. Microbiol.</span>, </em> <em><span itemtype="http://schema.org/PublicationVolume" itemscope="itemscope" itemprop="isPartOf"><span itemprop="volumeNumber">75 </span></span>(<span itemprop="issueNumber">4</span>):
<span itemprop="pagination">1197--1200</span></em> </span>(<em><span>February 2009<meta content="February 2009" itemprop="datePublished"/></span></em>)</span>Fri Feb 09 13:18:17 CET 2018Appl. Environ. Microbiol.feb41197--1200L-valine production during growth of pyruvate dehydrogenase complex-deficient {Corynebacterium} glutamicum in the presence of ethanol or by inactivation of the transcriptional regulator {SugR}752009Complex Corynebacterium Dehydrogenase Ethanol, Factors, Gene Knockout Pyruvate Techniques, Transcription Valine, glutamicum, myown Pyruvate dehydrogenase complex-deficient strains of Corynebacterium glutamicum produce L-valine from glucose only after depletion of the acetate required for growth. Here we show that inactivation of the DeoR-type transcriptional regulator SugR or replacement of acetate by ethanol already in course of the growth phase results in efficient L-valine production.Corynebacterium glutamicum tailored for high-yield L-valine productionhttps://puma.ub.uni-stuttgart.de/bibtex/29723d6ce30daaf4910269ea54837c2d7/bastianbastian2018-02-09T13:18:17+01:00Bacterial Biomass, Biosynthetic Complex, Corynebacterium Dehydrogenase Engineering, Expression, Fermentation Gene Genetic Ketol-Acid Oxidoreductases, Pathways, Proteins, Pyruvate Reductoisomerase, Transaminases, Valine, glutamicum, myown <span data-person-type="author" class="authorEditorList "><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bastian Blombach" itemprop="url" href="/person/1e1d424fe4d9f617e1a0837a7567bea3a/author/0"><span itemprop="name">B. Blombach</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Mark E. Schreiner" itemprop="url" href="/person/1e1d424fe4d9f617e1a0837a7567bea3a/author/1"><span itemprop="name">M. Schreiner</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Tobias Bartek" itemprop="url" href="/person/1e1d424fe4d9f617e1a0837a7567bea3a/author/2"><span itemprop="name">T. Bartek</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Marco Oldiges" itemprop="url" href="/person/1e1d424fe4d9f617e1a0837a7567bea3a/author/3"><span itemprop="name">M. Oldiges</span></a></span>, </span> and <span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bernhard J. Eikmanns" itemprop="url" href="/person/1e1d424fe4d9f617e1a0837a7567bea3a/author/4"><span itemprop="name">B. Eikmanns</span></a></span></span>. </span><span class="additional-entrytype-information"><span itemtype="http://schema.org/PublicationIssue" itemscope="itemscope" itemprop="isPartOf"><em><span itemprop="journal">Appl. Microbiol. Biotechnol.</span>, </em> <em><span itemtype="http://schema.org/PublicationVolume" itemscope="itemscope" itemprop="isPartOf"><span itemprop="volumeNumber">79 </span></span>(<span itemprop="issueNumber">3</span>):
<span itemprop="pagination">471--479</span></em> </span>(<em><span>June 2008<meta content="June 2008" itemprop="datePublished"/></span></em>)</span>Fri Feb 09 13:18:17 CET 2018Appl. Microbiol. Biotechnol.jun3471--479Corynebacterium glutamicum tailored for high-yield {L}-valine production792008Bacterial Biomass, Biosynthetic Complex, Corynebacterium Dehydrogenase Engineering, Expression, Fermentation Gene Genetic Ketol-Acid Oxidoreductases, Pathways, Proteins, Pyruvate Reductoisomerase, Transaminases, Valine, glutamicum, myown We recently engineered the wild type of Corynebacterium glutamicum for the growth-decoupled production of L: -valine from glucose by inactivation of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. Based on the first generation of pyruvate-dehydrogenase-complex-deficient C. glutamicum strains, a second generation of high-yield L-valine producers was constructed by successive deletion of the genes encoding pyruvate:quinone oxidoreductase, phosphoglucose isomerase, and pyruvate carboxylase and overexpression of ilvBNCE. In fed-batch fermentations at high cell densities, the newly constructed strains produced up to 410 mM (48 g/l) L-valine, showed a maximum yield of 0.75 to 0.86 mol/mol (0.49 to 0.56 g/g) of glucose in the production phase and, in contrast to the first generation strains, excreted neither pyruvate nor any other by-product tested.Platform engineering of Corynebacterium glutamicum with reduced pyruvate dehydrogenase complex activity for improved production of L-lysine, L-valine, and 2-ketoisovaleratehttps://puma.ub.uni-stuttgart.de/bibtex/2fc097b674eaff7966119cba97aaacd40/bastianbastian2018-02-09T13:18:17+01:00Acids, Biomass, Complex, Corynebacterium Dehydrogenase Deletion, Down-Regulation, Engineering Expression, Gene Genetic, Glucose, Keto Lysine, Metabolic Networks Pathways, Promoter Pyruvate Recombination, Regions, Valine, and glutamicum, myown <span data-person-type="author" class="authorEditorList "><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Jens Buchholz" itemprop="url" href="/person/1671300cf6c01886cf28889b6f3b9a19e/author/0"><span itemprop="name">J. Buchholz</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Andreas Schwentner" itemprop="url" href="/person/1671300cf6c01886cf28889b6f3b9a19e/author/1"><span itemprop="name">A. Schwentner</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Britta Brunnenkan" itemprop="url" href="/person/1671300cf6c01886cf28889b6f3b9a19e/author/2"><span itemprop="name">B. Brunnenkan</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Christina Gabris" itemprop="url" href="/person/1671300cf6c01886cf28889b6f3b9a19e/author/3"><span itemprop="name">C. Gabris</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Simon Grimm" itemprop="url" href="/person/1671300cf6c01886cf28889b6f3b9a19e/author/4"><span itemprop="name">S. Grimm</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Robert Gerstmeir" itemprop="url" href="/person/1671300cf6c01886cf28889b6f3b9a19e/author/5"><span itemprop="name">R. Gerstmeir</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Ralf Takors" itemprop="url" href="/person/1671300cf6c01886cf28889b6f3b9a19e/author/6"><span itemprop="name">R. Takors</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bernhard J. Eikmanns" itemprop="url" href="/person/1671300cf6c01886cf28889b6f3b9a19e/author/7"><span itemprop="name">B. Eikmanns</span></a></span>, </span> and <span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bastian Blombach" itemprop="url" href="/person/1671300cf6c01886cf28889b6f3b9a19e/author/8"><span itemprop="name">B. Blombach</span></a></span></span>. </span><span class="additional-entrytype-information"><span itemtype="http://schema.org/PublicationIssue" itemscope="itemscope" itemprop="isPartOf"><em><span itemprop="journal">Appl. Environ. Microbiol.</span>, </em> <em><span itemtype="http://schema.org/PublicationVolume" itemscope="itemscope" itemprop="isPartOf"><span itemprop="volumeNumber">79 </span></span>(<span itemprop="issueNumber">18</span>):
<span itemprop="pagination">5566--5575</span></em> </span>(<em><span>September 2013<meta content="September 2013" itemprop="datePublished"/></span></em>)</span>Fri Feb 09 13:18:17 CET 2018Appl. Environ. Microbiol.sep185566--5575Platform engineering of {Corynebacterium} glutamicum with reduced pyruvate dehydrogenase complex activity for improved production of {L}-lysine, {L}-valine, and 2-ketoisovalerate792013Acids, Biomass, Complex, Corynebacterium Dehydrogenase Deletion, Down-Regulation, Engineering Expression, Gene Genetic, Glucose, Keto Lysine, Metabolic Networks Pathways, Promoter Pyruvate Recombination, Regions, Valine, and glutamicum, myown Exchange of the native Corynebacterium glutamicum promoter of the aceE gene, encoding the E1p subunit of the pyruvate dehydrogenase complex (PDHC), with mutated dapA promoter variants led to a series of C. glutamicum strains with gradually reduced growth rates and PDHC activities. Upon overexpression of the l-valine biosynthetic genes ilvBNCE, all strains produced l-valine. Among these strains, C. glutamicum aceE A16 (pJC4 ilvBNCE) showed the highest biomass and product yields, and thus it was further improved by additional deletion of the pqo and ppc genes, encoding pyruvate:quinone oxidoreductase and phosphoenolpyruvate carboxylase, respectively. In fed-batch fermentations at high cell densities, C. glutamicum aceE A16 Δpqo Δppc (pJC4 ilvBNCE) produced up to 738 mM (i.e., 86.5 g/liter) l-valine with an overall yield (YP/S) of 0.36 mol per mol of glucose and a volumetric productivity (QP) of 13.6 mM per h [1.6 g/(liter × h)]. Additional inactivation of the transaminase B gene (ilvE) and overexpression of ilvBNCD instead of ilvBNCE transformed the l-valine-producing strain into a 2-ketoisovalerate producer, excreting up to 303 mM (35 g/liter) 2-ketoisovalerate with a YP/S of 0.24 mol per mol of glucose and a QP of 6.9 mM per h [0.8 g/(liter × h)]. The replacement of the aceE promoter by the dapA-A16 promoter in the two C. glutamicum l-lysine producers DM1800 and DM1933 improved the production by 100\% and 44\%, respectively. These results demonstrate that C. glutamicum strains with reduced PDHC activity are an excellent platform for the production of pyruvate-derived products.