PUMA publications for /user/bastian/Dehydrogenase%20gene%20corynebacterium%20bacterial,%20pyruvatehttps://puma.ub.uni-stuttgart.de/user/bastian/Dehydrogenase%20gene%20corynebacterium%20bacterial,%20pyruvatePUMA RSS feed for /user/bastian/Dehydrogenase%20gene%20corynebacterium%20bacterial,%20pyruvate2024-03-29T01:37:03+01:00Effect of pyruvate dehydrogenase complex deficiency on L-lysine production with Corynebacterium glutamicumhttps://puma.ub.uni-stuttgart.de/bibtex/2930ecfe113b73f241ab0452b05337d89/bastianbastian2018-02-09T13:18:17+01:00Bacterial, Base Biotechnology Complex, Corynebacterium DNA, Dehydrogenase Deletion, Expression, Fermentation, Gene Genes, Kinetics, Lysine, Pyruvate Sequence, glutamicum, myown <span data-person-type="author" class="authorEditorList "><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bastian Blombach" itemprop="url" href="/person/197097cd65b8e231a2578efbc7586a5dc/author/0"><span itemprop="name">B. Blombach</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Mark E. Schreiner" itemprop="url" href="/person/197097cd65b8e231a2578efbc7586a5dc/author/1"><span itemprop="name">M. Schreiner</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Matthias Moch" itemprop="url" href="/person/197097cd65b8e231a2578efbc7586a5dc/author/2"><span itemprop="name">M. Moch</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Marco Oldiges" itemprop="url" href="/person/197097cd65b8e231a2578efbc7586a5dc/author/3"><span itemprop="name">M. Oldiges</span></a></span>, </span> and <span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bernhard J. Eikmanns" itemprop="url" href="/person/197097cd65b8e231a2578efbc7586a5dc/author/4"><span itemprop="name">B. Eikmanns</span></a></span></span>. </span><span class="additional-entrytype-information"><span itemtype="http://schema.org/PublicationIssue" itemscope="itemscope" itemprop="isPartOf"><em><span itemprop="journal">Appl. Microbiol. Biotechnol.</span>, </em> <em><span itemtype="http://schema.org/PublicationVolume" itemscope="itemscope" itemprop="isPartOf"><span itemprop="volumeNumber">76 </span></span>(<span itemprop="issueNumber">3</span>):
<span itemprop="pagination">615--623</span></em> </span>(<em><span>September 2007<meta content="September 2007" itemprop="datePublished"/></span></em>)</span>Fri Feb 09 13:18:17 CET 2018Appl. Microbiol. Biotechnol.sep3615--623Effect of pyruvate dehydrogenase complex deficiency on {L}-lysine production with {Corynebacterium} glutamicum762007Bacterial, Base Biotechnology Complex, Corynebacterium DNA, Dehydrogenase Deletion, Expression, Fermentation, Gene Genes, Kinetics, Lysine, Pyruvate Sequence, glutamicum, myown Intracellular precursor supply is a critical factor for amino acid productivity of Corynebacterium glutamicum. To test for the effect of improved pyruvate availability on L-lysine production, we deleted the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex (PDHC) in the L-lysine-producer C. glutamicum DM1729 and characterised the resulting strain DM1729-BB1 for growth and L-lysine production. Compared to the host strain, C. glutamicum DM1729-BB1 showed no PDHC activity, was acetate auxotrophic and, after complete consumption of the available carbon sources glucose and acetate, showed a more than 50\% lower substrate-specific biomass yield (0.14 vs 0.33 mol C/mol C), an about fourfold higher biomass-specific L-lysine yield (5.27 vs 1.23 mmol/g cell dry weight) and a more than 40\% higher substrate-specific L-lysine yield (0.13 vs 0.09 mol C/mol C). Overexpression of the pyruvate carboxylase or diaminopimelate dehydrogenase genes in C. glutamicum DM1729-BB1 resulted in a further increase in the biomass-specific L-lysine yield by 6 and 56\%, respectively. In addition to L-lysine, significant amounts of pyruvate, L-alanine and L-valine were produced by C. glutamicum DM1729-BB1 and its derivatives, suggesting a surplus of precursor availability and a further potential to improve L-lysine production by engineering the L-lysine biosynthetic pathway.RamB is an activator of the pyruvate dehydrogenase complex subunit E1p gene in Corynebacterium glutamicumhttps://puma.ub.uni-stuttgart.de/bibtex/2751d62556f83a4d54902c3b9fac85dfb/bastianbastian2018-02-09T13:18:17+01:00(Lipoamide) Acetates, Bacterial Bacterial, Base Corynebacterium Data, Dehydrogenase Expression Gene Molecular Proteins, Pyruvate Regulation, Sequence Sequence, glutamicum, myown <span data-person-type="author" class="authorEditorList "><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bastian Blombach" itemprop="url" href="/person/19f5f5ec2cc8c58eb2101574f015cd25b/author/0"><span itemprop="name">B. Blombach</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Annette Cramer" itemprop="url" href="/person/19f5f5ec2cc8c58eb2101574f015cd25b/author/1"><span itemprop="name">A. Cramer</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bernhard J. Eikmanns" itemprop="url" href="/person/19f5f5ec2cc8c58eb2101574f015cd25b/author/2"><span itemprop="name">B. Eikmanns</span></a></span>, </span> and <span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Mark Schreiner" itemprop="url" href="/person/19f5f5ec2cc8c58eb2101574f015cd25b/author/3"><span itemprop="name">M. Schreiner</span></a></span></span>. </span><span class="additional-entrytype-information"><span itemtype="http://schema.org/PublicationIssue" itemscope="itemscope" itemprop="isPartOf"><em><span itemprop="journal">J. Mol. Microbiol. Biotechnol.</span>, </em> <em><span itemtype="http://schema.org/PublicationVolume" itemscope="itemscope" itemprop="isPartOf"><span itemprop="volumeNumber">16 </span></span>(<span itemprop="issueNumber">3-4</span>):
<span itemprop="pagination">236--239</span></em> </span>(<em><span>2009<meta content="2009" itemprop="datePublished"/></span></em>)</span>Fri Feb 09 13:18:17 CET 2018J. Mol. Microbiol. Biotechnol.3-4236--239{RamB} is an activator of the pyruvate dehydrogenase complex subunit {E}1p gene in {Corynebacterium} glutamicum162009(Lipoamide) Acetates, Bacterial Bacterial, Base Corynebacterium Data, Dehydrogenase Expression Gene Molecular Proteins, Pyruvate Regulation, Sequence Sequence, glutamicum, myown In Corynebacterium glutamicum, the transcriptional regulator RamB negatively controls the expression of genes involved in acetate metabolism. Here we show that during growth in media containing glucose and in complex medium without glucose RamB activates expression of the aceE gene, encoding the E1p subunit of the pyruvate dehydrogenase complex. Thus, RamB functions both as repressor and as activator in C. glutamicum.