PUMA publications for /user/bastian/Bicarbonates,https://puma.ub.uni-stuttgart.de/user/bastian/Bicarbonates,PUMA RSS feed for /user/bastian/Bicarbonates,2024-03-28T18:40:20+01:00CO₂ /HCO₃⁻ perturbations of simulated large scale gradients in a scale-down device cause fast transcriptional responses in Corynebacterium glutamicumhttps://puma.ub.uni-stuttgart.de/bibtex/20f156189f5ad943ea875c8e9d001792c/bastianbastian2018-02-09T13:18:17+01:00Bacterial, Bicarbonates, Bioreactors, Carbon Catechol Corynebacterium Culture Dioxide, Expression Gene Genetic, Inhibitors Lysine, Media, O-Methyltransferase Profiling, Regulation, Transcription, glutamicum, myown <span data-person-type="author" class="authorEditorList "><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Jens Buchholz" itemprop="url" href="/person/19ac7a3d4aa2c97efbb1a2a7bd96710fc/author/0"><span itemprop="name">J. Buchholz</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Michaela Graf" itemprop="url" href="/person/19ac7a3d4aa2c97efbb1a2a7bd96710fc/author/1"><span itemprop="name">M. Graf</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Andreas Freund" itemprop="url" href="/person/19ac7a3d4aa2c97efbb1a2a7bd96710fc/author/2"><span itemprop="name">A. Freund</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Tobias Busche" itemprop="url" href="/person/19ac7a3d4aa2c97efbb1a2a7bd96710fc/author/3"><span itemprop="name">T. Busche</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Jörn Kalinowski" itemprop="url" href="/person/19ac7a3d4aa2c97efbb1a2a7bd96710fc/author/4"><span itemprop="name">J. Kalinowski</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bastian Blombach" itemprop="url" href="/person/19ac7a3d4aa2c97efbb1a2a7bd96710fc/author/5"><span itemprop="name">B. Blombach</span></a></span>, </span> and <span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Ralf Takors" itemprop="url" href="/person/19ac7a3d4aa2c97efbb1a2a7bd96710fc/author/6"><span itemprop="name">R. Takors</span></a></span></span>. </span><span class="additional-entrytype-information"><span itemtype="http://schema.org/PublicationIssue" itemscope="itemscope" itemprop="isPartOf"><em><span itemprop="journal">Appl. Microbiol. Biotechnol.</span>, </em> <em><span itemtype="http://schema.org/PublicationVolume" itemscope="itemscope" itemprop="isPartOf"><span itemprop="volumeNumber">98 </span></span>(<span itemprop="issueNumber">20</span>):
<span itemprop="pagination">8563--8572</span></em> </span>(<em><span>October 2014<meta content="October 2014" itemprop="datePublished"/></span></em>)</span>Fri Feb 09 13:18:17 CET 2018Appl. Microbiol. Biotechnol.oct208563--8572{CO}₂ /{HCO}₃⁻ perturbations of simulated large scale gradients in a scale-down device cause fast transcriptional responses in {Corynebacterium} glutamicum982014Bacterial, Bicarbonates, Bioreactors, Carbon Catechol Corynebacterium Culture Dioxide, Expression Gene Genetic, Inhibitors Lysine, Media, O-Methyltransferase Profiling, Regulation, Transcription, glutamicum, myown The exploration of scale-down models to imitate the influence of large scale bioreactor inhomogeneities on cellular metabolism is a topic with increasing relevance. While gradients of substrates, pH, or dissolved oxygen are often investigated, oscillating CO2/HCO3 (-) levels, a typical scenario in large industrial bioreactors, is rarely addressed. Hereby, we investigate the metabolic and transcriptional response in Corynebacterium glutamicum wild type as well as the impact on L-lysine production in a model strain exposed to pCO2 gradients of (75-315) mbar. A three-compartment cascade bioreactor system was developed and characterized that offers high flexibility for installing gradients and residence times to mimic industrial-relevant conditions and provides the potential of accurate carbon balancing. The phenomenological analysis of cascade fermentations imposed to the pCO2 gradients at industry-relevant residence times of about 3.6 min did not significantly impair the process performance, with growth and product formation being similar to control conditions. However, transcriptional analysis disclosed up to 66 differentially expressed genes already after 3.6 min under stimulus exposure, with the overall change in gene expression directly correlateable to the pCO2 gradient intensity and the residence time of the cells.