PUMA publications for /tag/fungal%20Glucose,https://puma.ub.uni-stuttgart.de/tag/fungal%20Glucose,PUMA RSS feed for /tag/fungal%20Glucose,2024-03-28T11:43:08+01:00Corynebacterium glutamicum tailored for efficient isobutanol productionhttps://puma.ub.uni-stuttgart.de/bibtex/2a0a5fcfba6017eb6a61292baef557af7/bastianbastian2018-02-09T13:18:17+01:00Anaerobiosis, Bacterial Bacterial, Butanols, Chromosomes, Corynebacterium Escherichia Fungal Glucose, Lactococcus Metabolic Networks Pathways, Plasmids, Proteins Proteins, Recombinant Saccharomyces and cerevisiae, coli, glutamicum, lactis, myown <span data-person-type="author" class="authorEditorList "><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bastian Blombach" itemprop="url" href="/person/10ff226fcd2ff0614810a1890f8f46f8e/author/0"><span itemprop="name">B. Blombach</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Tanja Riester" itemprop="url" href="/person/10ff226fcd2ff0614810a1890f8f46f8e/author/1"><span itemprop="name">T. Riester</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Stefan Wieschalka" itemprop="url" href="/person/10ff226fcd2ff0614810a1890f8f46f8e/author/2"><span itemprop="name">S. Wieschalka</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Christian Ziert" itemprop="url" href="/person/10ff226fcd2ff0614810a1890f8f46f8e/author/3"><span itemprop="name">C. Ziert</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Jung-Won Youn" itemprop="url" href="/person/10ff226fcd2ff0614810a1890f8f46f8e/author/4"><span itemprop="name">J. Youn</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Volker F. Wendisch" itemprop="url" href="/person/10ff226fcd2ff0614810a1890f8f46f8e/author/5"><span itemprop="name">V. Wendisch</span></a></span>, </span> and <span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bernhard J. Eikmanns" itemprop="url" href="/person/10ff226fcd2ff0614810a1890f8f46f8e/author/6"><span itemprop="name">B. Eikmanns</span></a></span></span>. </span><span class="additional-entrytype-information"><span itemtype="http://schema.org/PublicationIssue" itemscope="itemscope" itemprop="isPartOf"><em><span itemprop="journal">Appl. Environ. Microbiol.</span>, </em> <em><span itemtype="http://schema.org/PublicationVolume" itemscope="itemscope" itemprop="isPartOf"><span itemprop="volumeNumber">77 </span></span>(<span itemprop="issueNumber">10</span>):
<span itemprop="pagination">3300--3310</span></em> </span>(<em><span>May 2011<meta content="May 2011" itemprop="datePublished"/></span></em>)</span>Fri Feb 09 13:18:17 CET 2018Appl. Environ. Microbiol.may103300--3310Corynebacterium glutamicum tailored for efficient isobutanol production772011Anaerobiosis, Bacterial Bacterial, Butanols, Chromosomes, Corynebacterium Escherichia Fungal Glucose, Lactococcus Metabolic Networks Pathways, Plasmids, Proteins Proteins, Recombinant Saccharomyces and cerevisiae, coli, glutamicum, lactis, myown We recently engineered Corynebacterium glutamicum for aerobic production of 2-ketoisovalerate by inactivation of the pyruvate dehydrogenase complex, pyruvate:quinone oxidoreductase, transaminase B, and additional overexpression of the ilvBNCD genes, encoding acetohydroxyacid synthase, acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. Based on this strain, we engineered C. glutamicum for the production of isobutanol from glucose under oxygen deprivation conditions by inactivation of l-lactate and malate dehydrogenases, implementation of ketoacid decarboxylase from Lactococcus lactis, alcohol dehydrogenase 2 (ADH2) from Saccharomyces cerevisiae, and expression of the pntAB transhydrogenase genes from Escherichia coli. The resulting strain produced isobutanol with a substrate-specific yield (Y(P/S)) of 0.60 ± 0.02 mol per mol of glucose. Interestingly, a chromosomally encoded alcohol dehydrogenase rather than the plasmid-encoded ADH2 from S. cerevisiae was involved in isobutanol formation with C. glutamicum, and overexpression of the corresponding adhA gene increased the Y(P/S) to 0.77 ± 0.01 mol of isobutanol per mol of glucose. Inactivation of the malic enzyme significantly reduced the Y(P/S), indicating that the metabolic cycle consisting of pyruvate and/or phosphoenolpyruvate carboxylase, malate dehydrogenase, and malic enzyme is responsible for the conversion of NADH + H+ to NADPH + H+. In fed-batch fermentations with an aerobic growth phase and an oxygen-depleted production phase, the most promising strain, C. glutamicum ΔaceE Δpqo ΔilvE ΔldhA Δmdh(pJC4ilvBNCD-pntAB)(pBB1kivd-adhA), produced about 175 mM isobutanol, with a volumetric productivity of 4.4 mM h⁻¹, and showed an overall Y(P/S) of about 0.48 mol per mol of glucose in the production phase.