PUMA publications for /tag/anaerobiosis,%20Dioxidehttps://puma.ub.uni-stuttgart.de/tag/anaerobiosis,%20DioxidePUMA RSS feed for /tag/anaerobiosis,%20Dioxide2024-03-19T02:55:52+01:00Carbon flux analysis by 13C nuclear magnetic resonance to determine the effect of CO2 on anaerobic succinate production by Corynebacterium glutamicumhttps://puma.ub.uni-stuttgart.de/bibtex/22e74e76dec66d069069ac7fbe5bedf94/bastianbastian2018-02-09T13:18:17+01:00Acid, Anaerobiosis, Carbon Corynebacterium Dioxide Glucose, Isotope Isotopes, Labeling, Magnetic Resonance Spectroscopy, Succinic glutamicum, myown <span data-person-type="author" class="authorEditorList "><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Dušica Radoš" itemprop="url" href="/person/138e7d0611f298c2eedc8bd9086a28955/author/0"><span itemprop="name">D. Radoš</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="David L. Turner" itemprop="url" href="/person/138e7d0611f298c2eedc8bd9086a28955/author/1"><span itemprop="name">D. Turner</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Luís L. Fonseca" itemprop="url" href="/person/138e7d0611f298c2eedc8bd9086a28955/author/2"><span itemprop="name">L. Fonseca</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Ana Lúcia Carvalho" itemprop="url" href="/person/138e7d0611f298c2eedc8bd9086a28955/author/3"><span itemprop="name">A. Carvalho</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bastian Blombach" itemprop="url" href="/person/138e7d0611f298c2eedc8bd9086a28955/author/4"><span itemprop="name">B. Blombach</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Bernhard J. Eikmanns" itemprop="url" href="/person/138e7d0611f298c2eedc8bd9086a28955/author/5"><span itemprop="name">B. Eikmanns</span></a></span>, </span><span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Ana Rute Neves" itemprop="url" href="/person/138e7d0611f298c2eedc8bd9086a28955/author/6"><span itemprop="name">A. Neves</span></a></span>, </span> and <span><span itemtype="http://schema.org/Person" itemscope="itemscope" itemprop="author"><a title="Helena Santos" itemprop="url" href="/person/138e7d0611f298c2eedc8bd9086a28955/author/7"><span itemprop="name">H. Santos</span></a></span></span>. </span><span class="additional-entrytype-information"><span itemtype="http://schema.org/PublicationIssue" itemscope="itemscope" itemprop="isPartOf"><em><span itemprop="journal">Appl. Environ. Microbiol.</span>, </em> <em><span itemtype="http://schema.org/PublicationVolume" itemscope="itemscope" itemprop="isPartOf"><span itemprop="volumeNumber">80 </span></span>(<span itemprop="issueNumber">10</span>):
<span itemprop="pagination">3015--3024</span></em> </span>(<em><span>May 2014<meta content="May 2014" itemprop="datePublished"/></span></em>)</span>Fri Feb 09 13:18:17 CET 2018Appl. Environ. Microbiol.may103015--3024Carbon flux analysis by 13C nuclear magnetic resonance to determine the effect of {CO}2 on anaerobic succinate production by {Corynebacterium} glutamicum802014Acid, Anaerobiosis, Carbon Corynebacterium Dioxide Glucose, Isotope Isotopes, Labeling, Magnetic Resonance Spectroscopy, Succinic glutamicum, myown Wild-type Corynebacterium glutamicum produces a mixture of lactic, succinic, and acetic acids from glucose under oxygen deprivation. We investigated the effect of CO2 on the production of organic acids in a two-stage process: cells were grown aerobically in glucose, and subsequently, organic acid production by nongrowing cells was studied under anaerobic conditions. The presence of CO2 caused up to a 3-fold increase in the succinate yield (1 mol per mol of glucose) and about 2-fold increase in acetate, both at the expense of l-lactate production; moreover, dihydroxyacetone formation was abolished. The redistribution of carbon fluxes in response to CO2 was estimated by using (13)C-labeled glucose and (13)C nuclear magnetic resonance (NMR) analysis of the labeling patterns in end products. The flux analysis showed that 97\% of succinate was produced via the reductive part of the tricarboxylic acid cycle, with the low activity of the oxidative branch being sufficient to provide the reducing equivalents needed for the redox balance. The flux via the pentose phosphate pathway was low ({\textasciitilde}5\%) regardless of the presence or absence of CO2. Moreover, there was significant channeling of carbon to storage compounds (glycogen and trehalose) and concomitant catabolism of these reserves. The intracellular and extracellular pools of lactate and succinate were measured by in vivo NMR, and the stoichiometry (H(+):organic acid) of the respective exporters was calculated. This study shows that it is feasible to take advantage of natural cellular regulation mechanisms to obtain high yields of succinate with C. glutamicum without genetic manipulation.