%0 Book Section %1 Siegemund2014 %A Siegemund, Martin %A Richter, Fabian %A Seifert, Oliver %A Unverdorben, Felix %A Kontermann, Roland E. %B Methods in molecular biology (Clifton, N.J.) %D 2014 %K 2014 izi kontermann %P 273--295 %R 10.1007/978-1-62703-992-5_18 %T Expression and Purification of Recombinant Antibody Formats and Antibody Fusion Proteins %U https://doi.org/10.1007/978-1-62703-992-5_18 %V 1131 %X In the laboratory-scale production of antibody fragments or antibody fusion proteins, it is often difficult to keep track on the most suitable affinity tags for protein purification from either prokaryotic or eukaryotic host systems. Here, we describe how such recombinant proteins derived from Escherichia coli lysates as well as HEK293 cell culture supernatants are purified by IMAC and by different affinity chromatography methods based on fusions to FLAG-tag, Strep-tag, and Fc domains.

@incollection{Siegemund2014, abstract = {In the laboratory-scale production of antibody fragments or antibody fusion proteins, it is often difficult to keep track on the most suitable affinity tags for protein purification from either prokaryotic or eukaryotic host systems. Here, we describe how such recombinant proteins derived from Escherichia coli lysates as well as HEK293 cell culture supernatants are purified by IMAC and by different affinity chromatography methods based on fusions to FLAG-tag, Strep-tag, and Fc domains.}, added-at = {2023-06-29T13:07:55.000+0200}, author = {Siegemund, Martin and Richter, Fabian and Seifert, Oliver and Unverdorben, Felix and Kontermann, Roland E.}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/2b0d96f2155578a8bd39321d5f76833c7/fabian}, booktitle = {Methods in molecular biology (Clifton, N.J.)}, doi = {10.1007/978-1-62703-992-5_18}, interhash = {b60886559d06c01430c6953275983388}, intrahash = {b0d96f2155578a8bd39321d5f76833c7}, issn = {1940-6029}, keywords = {2014 izi kontermann}, pages = {273--295}, pmid = {24515473}, timestamp = {2023-06-29T13:07:55.000+0200}, title = {{Expression and Purification of Recombinant Antibody Formats and Antibody Fusion Proteins}}, url = {https://doi.org/10.1007/978-1-62703-992-5_18}, volume = 1131, year = 2014 }