Abstract
A family of 10 competing, unstructured models has been developed to
model cell growth, substrate consumption, and product formation of the
pyruvate producing strain Escherichia coli YYC202 ldhA::Kan strain used
in fed-batch processes. The strain is completely blocked in its ability
to convert pyruvate into acetyl-CoA or acetate (using glucose as the
carbon source) resulting in an acetate auxotrophy during growth in
glucose minimal medium. Parameter estimation was carried out using data
from fed-batch fermentation performed at constant glucose feed rates of
q(VG)=10 mL h(-1). Acetate was fed according to the previously developed
feeding strategy. While the model identification was realized by
least-square fit, the model discrimination was based on the model
selection criterion (MSC). The validation of model parameters was
performed applying data from two different fed-batch experiments with
glucose feed rate q(VG)=20 and 30 mL h(-1), respectively. Consequently,
the most suitable model was identified that reflected the pyruvate and
biomass curves adequately by considering a pyruvate inhibited growth
(Jerusalimsky approach) and pyruvate inhibited product formation
(described by modified Luedeking-Piret/Levenspiel term).
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