%0 Journal Article %1 Eiseler2007 %A Eiseler, Tim %A Schmid, Michael A. %A Topbas, Fitnat %A Pfizenmaier, Klaus %A Hausser, Angelika %D 2007 %J FEBS Letters %K 2007 hausser izi pfizenmaier %N 22 %P 4279--4287 %R 10.1016/j.febslet.2007.07.079 %T PKD is recruited to sites of actin remodelling at the leading edge and negatively regulates cell migration %U https://www.ncbi.nlm.nih.gov/pubmed/17707375 %V 581 %X Protein kinase D (PKD) has been implicated in the regulation of cell shape, adhesion, and migration. At the leading edge of migrating cells active PKD co-localizes with F-actin, Arp3 and cortactin. Platelet derived growth factor (PDGF) activates PKD and recruits the kinase to the leading edge, suggesting a role for PKD in actin remodelling. In support of this, PKD directly interacts with F-actin and phosphorylates cortactin in vitro. Interference with PKD function by overexpression of a dominant negative PKD or by PKD-specific siRNA enhanced cell migration, whereas cells overexpressing PKD wild type displayed reduced migratory potential. Taken together, these data reveal a negative regulatory function of PKD in cell migration. © 2007 Federation of European Biochemical Societies. %Z Eiseler, TimSchmid, Michael ATopbas, FitnatPfizenmaier, KlausHausser, AngelikaengResearch Support, Non-U.S. Gov'tEnglandFEBS Lett. 2007 Sep 4;581(22):4279-87. doi: 10.1016/j.febslet.2007.07.079. Epub 2007 Aug 10. %7 2007/08/21 %@ 0014-5793 (Print)$\backslash$r0014-5793 (Linking)

@article{Eiseler2007, abstract = {Protein kinase D (PKD) has been implicated in the regulation of cell shape, adhesion, and migration. At the leading edge of migrating cells active PKD co-localizes with F-actin, Arp3 and cortactin. Platelet derived growth factor (PDGF) activates PKD and recruits the kinase to the leading edge, suggesting a role for PKD in actin remodelling. In support of this, PKD directly interacts with F-actin and phosphorylates cortactin in vitro. Interference with PKD function by overexpression of a dominant negative PKD or by PKD-specific siRNA enhanced cell migration, whereas cells overexpressing PKD wild type displayed reduced migratory potential. Taken together, these data reveal a negative regulatory function of PKD in cell migration. {\textcopyright} 2007 Federation of European Biochemical Societies.}, added-at = {2023-06-29T13:07:55.000+0200}, annote = {Eiseler, TimSchmid, Michael ATopbas, FitnatPfizenmaier, KlausHausser, AngelikaengResearch Support, Non-U.S. Gov'tEnglandFEBS Lett. 2007 Sep 4;581(22):4279-87. doi: 10.1016/j.febslet.2007.07.079. Epub 2007 Aug 10.}, author = {Eiseler, Tim and Schmid, Michael A. and Topbas, Fitnat and Pfizenmaier, Klaus and Hausser, Angelika}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/2ee14d3a1862f9d0be1bf8b95e06fcde1/fabian}, doi = {10.1016/j.febslet.2007.07.079}, edition = {2007/08/21}, interhash = {2a14426ea713a0dcfa2b60b429ce51b1}, intrahash = {ee14d3a1862f9d0be1bf8b95e06fcde1}, isbn = {0014-5793 (Print)$\backslash$r0014-5793 (Linking)}, issn = {00145793}, journal = {FEBS Letters}, keywords = {2007 hausser izi pfizenmaier}, number = 22, pages = {4279--4287}, pmid = {17707375}, timestamp = {2023-06-29T13:07:55.000+0200}, title = {{PKD is recruited to sites of actin remodelling at the leading edge and negatively regulates cell migration}}, url = {https://www.ncbi.nlm.nih.gov/pubmed/17707375}, volume = 581, year = 2007 }