We recently engineered the wild type of Corynebacterium glutamicum for the growth-decoupled production of L: -valine from glucose by inactivation of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. Based on the first generation of pyruvate-dehydrogenase-complex-deficient C. glutamicum strains, a second generation of high-yield L-valine producers was constructed by successive deletion of the genes encoding pyruvate:quinone oxidoreductase, phosphoglucose isomerase, and pyruvate carboxylase and overexpression of ilvBNCE. In fed-batch fermentations at high cell densities, the newly constructed strains produced up to 410 mM (48 g/l) L-valine, showed a maximum yield of 0.75 to 0.86 mol/mol (0.49 to 0.56 g/g) of glucose in the production phase and, in contrast to the first generation strains, excreted neither pyruvate nor any other by-product tested.
%0 Journal Article
%1 blombach_corynebacterium_2008
%A Blombach, Bastian
%A Schreiner, Mark E.
%A Bartek, Tobias
%A Oldiges, Marco
%A Eikmanns, Bernhard J.
%D 2008
%J Appl. Microbiol. Biotechnol.
%K Bacterial Biomass, Biosynthetic Complex, Corynebacterium Dehydrogenase Engineering, Expression, Fermentation Gene Genetic Ketol-Acid Oxidoreductases, Pathways, Proteins, Pyruvate Reductoisomerase, Transaminases, Valine, glutamicum, myown
%N 3
%P 471--479
%R 10.1007/s00253-008-1444-z
%T Corynebacterium glutamicum tailored for high-yield L-valine production
%V 79
%X We recently engineered the wild type of Corynebacterium glutamicum for the growth-decoupled production of L: -valine from glucose by inactivation of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. Based on the first generation of pyruvate-dehydrogenase-complex-deficient C. glutamicum strains, a second generation of high-yield L-valine producers was constructed by successive deletion of the genes encoding pyruvate:quinone oxidoreductase, phosphoglucose isomerase, and pyruvate carboxylase and overexpression of ilvBNCE. In fed-batch fermentations at high cell densities, the newly constructed strains produced up to 410 mM (48 g/l) L-valine, showed a maximum yield of 0.75 to 0.86 mol/mol (0.49 to 0.56 g/g) of glucose in the production phase and, in contrast to the first generation strains, excreted neither pyruvate nor any other by-product tested.
@article{blombach_corynebacterium_2008,
abstract = {We recently engineered the wild type of Corynebacterium glutamicum for the growth-decoupled production of L: -valine from glucose by inactivation of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. Based on the first generation of pyruvate-dehydrogenase-complex-deficient C. glutamicum strains, a second generation of high-yield L-valine producers was constructed by successive deletion of the genes encoding pyruvate:quinone oxidoreductase, phosphoglucose isomerase, and pyruvate carboxylase and overexpression of ilvBNCE. In fed-batch fermentations at high cell densities, the newly constructed strains produced up to 410 mM (48 g/l) L-valine, showed a maximum yield of 0.75 to 0.86 mol/mol (0.49 to 0.56 g/g) of glucose in the production phase and, in contrast to the first generation strains, excreted neither pyruvate nor any other by-product tested.},
added-at = {2018-02-09T13:18:17.000+0100},
author = {Blombach, Bastian and Schreiner, Mark E. and Bartek, Tobias and Oldiges, Marco and Eikmanns, Bernhard J.},
biburl = {https://puma.ub.uni-stuttgart.de/bibtex/29723d6ce30daaf4910269ea54837c2d7/bastian},
doi = {10.1007/s00253-008-1444-z},
interhash = {e1d424fe4d9f617e1a0837a7567bea3a},
intrahash = {9723d6ce30daaf4910269ea54837c2d7},
issn = {0175-7598},
journal = {Appl. Microbiol. Biotechnol.},
keywords = {Bacterial Biomass, Biosynthetic Complex, Corynebacterium Dehydrogenase Engineering, Expression, Fermentation Gene Genetic Ketol-Acid Oxidoreductases, Pathways, Proteins, Pyruvate Reductoisomerase, Transaminases, Valine, glutamicum, myown},
language = {eng},
month = jun,
number = 3,
pages = {471--479},
pmid = {18379776},
timestamp = {2018-02-09T12:18:56.000+0100},
title = {Corynebacterium glutamicum tailored for high-yield {L}-valine production},
volume = 79,
year = 2008
}