%0 Journal Article %1 blombach_l-valine_2007 %A Blombach, Bastian %A Schreiner, Mark E. %A Holátko, Jirí %A Bartek, Tobias %A Oldiges, Marco %A Eikmanns, Bernhard J. %D 2007 %J Appl. Environ. Microbiol. %K Acid, Alanine Complex, Corynebacterium Dehydrogenase Fermentation, Isoleucine, Lysine, Pyruvate Pyruvic Valine, glutamicum, myown %N 7 %P 2079--2084 %R 10.1128/AEM.02826-06 %T L-valine production with pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum %V 73 %X Corynebacterium glutamicum was engineered for the production of L-valine from glucose by deletion of the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. In the absence of cellular growth, C. glutamicum DeltaaceE showed a relatively high intracellular concentration of pyruvate (25.9 mM) and produced significant amounts of pyruvate, L-alanine, and L-valine from glucose as the sole carbon source. Lactate or acetate was not formed. Plasmid-bound overexpression of ilvBNCE in C. glutamicum DeltaaceE resulted in an approximately 10-fold-lower intracellular pyruvate concentration (2.3 mM) and a shift of the extracellular product pattern from pyruvate and L-alanine towards L-valine. In fed-batch fermentations at high cell densities and an excess of glucose, C. glutamicum DeltaaceE(pJC4ilvBNCE) produced up to 210 mM L-valine with a volumetric productivity of 10.0 mM h(-1) (1.17 g l(-1) h(-1)) and a maximum yield of about 0.6 mol per mol (0.4 g per g) of glucose.

@article{blombach_l-valine_2007, abstract = {Corynebacterium glutamicum was engineered for the production of L-valine from glucose by deletion of the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. In the absence of cellular growth, C. glutamicum DeltaaceE showed a relatively high intracellular concentration of pyruvate (25.9 mM) and produced significant amounts of pyruvate, L-alanine, and L-valine from glucose as the sole carbon source. Lactate or acetate was not formed. Plasmid-bound overexpression of ilvBNCE in C. glutamicum DeltaaceE resulted in an approximately 10-fold-lower intracellular pyruvate concentration (2.3 mM) and a shift of the extracellular product pattern from pyruvate and L-alanine towards L-valine. In fed-batch fermentations at high cell densities and an excess of glucose, C. glutamicum DeltaaceE(pJC4ilvBNCE) produced up to 210 mM L-valine with a volumetric productivity of 10.0 mM h(-1) (1.17 g l(-1) h(-1)) and a maximum yield of about 0.6 mol per mol (0.4 g per g) of glucose.}, added-at = {2018-02-09T13:18:17.000+0100}, author = {Blombach, Bastian and Schreiner, Mark E. and Holátko, Jirí and Bartek, Tobias and Oldiges, Marco and Eikmanns, Bernhard J.}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/25bac540150e10acba07b2f8205f6f070/bastian}, doi = {10.1128/AEM.02826-06}, interhash = {d744d92c5af8f45409137c58d022ef1c}, intrahash = {5bac540150e10acba07b2f8205f6f070}, issn = {0099-2240}, journal = {Appl. Environ. Microbiol.}, keywords = {Acid, Alanine Complex, Corynebacterium Dehydrogenase Fermentation, Isoleucine, Lysine, Pyruvate Pyruvic Valine, glutamicum, myown}, language = {eng}, month = apr, number = 7, pages = {2079--2084}, pmcid = {PMC1855657}, pmid = {17293513}, timestamp = {2018-02-09T12:18:56.000+0100}, title = {L-valine production with pyruvate dehydrogenase complex-deficient {Corynebacterium} glutamicum}, volume = 73, year = 2007 }