Immunoliposomes generated by coupling of antibodies to the liposomal surface allow for an active tissue targeting, e.g., through binding to tumor cell-specific receptors. Instead of whole antibodies, single-chain Fv fragments (scFv), which represent the smallest part of an antibody containing the entire antigen-binding site, find increasing usage as targeting moiety. Here we provide protocols for the preparation of type II scFv immunoliposomes by the conventional coupling method as well as the post-insertion method. Furthermore protocols to analyze binding of these immunoliposomes to antigen-expressing cells as well as internalization through receptor-mediated endocytosis are included.
%0 Journal Article
%1 Rothdiener2010
%A Rothdiener, Miriam
%A Beuttler, Julia
%A Messerschmidt, S. K.
%A Kontermann, Roland E.
%D 2010
%J Methods in molecular biology (Clifton, N.J.)
%K 2010 izi kontermann
%P 295--308
%R 10.1007/978-1-60761-609-2_20
%T Antibody targeting of nanoparticles to tumor-specific receptors: immunoliposomes.
%U https://www.ncbi.nlm.nih.gov/pubmed/20217604
%V 624
%X Immunoliposomes generated by coupling of antibodies to the liposomal surface allow for an active tissue targeting, e.g., through binding to tumor cell-specific receptors. Instead of whole antibodies, single-chain Fv fragments (scFv), which represent the smallest part of an antibody containing the entire antigen-binding site, find increasing usage as targeting moiety. Here we provide protocols for the preparation of type II scFv immunoliposomes by the conventional coupling method as well as the post-insertion method. Furthermore protocols to analyze binding of these immunoliposomes to antigen-expressing cells as well as internalization through receptor-mediated endocytosis are included.
%Z Rothdiener, MiriamBeuttler, JuliaMesserschmidt, Sylvia K EKontermann, Roland EengMethods Mol Biol. 2010;624:295-308. doi: 10.1007/978-1-60761-609-2\_20.
%7 2010/03/11
%@ 978-1-60761-608-5
@article{Rothdiener2010,
abstract = {Immunoliposomes generated by coupling of antibodies to the liposomal surface allow for an active tissue targeting, e.g., through binding to tumor cell-specific receptors. Instead of whole antibodies, single-chain Fv fragments (scFv), which represent the smallest part of an antibody containing the entire antigen-binding site, find increasing usage as targeting moiety. Here we provide protocols for the preparation of type II scFv immunoliposomes by the conventional coupling method as well as the post-insertion method. Furthermore protocols to analyze binding of these immunoliposomes to antigen-expressing cells as well as internalization through receptor-mediated endocytosis are included.},
added-at = {2018-02-01T15:44:38.000+0100},
annote = {Rothdiener, MiriamBeuttler, JuliaMesserschmidt, Sylvia K EKontermann, Roland EengMethods Mol Biol. 2010;624:295-308. doi: 10.1007/978-1-60761-609-2{\_}20.},
author = {Rothdiener, Miriam and Beuttler, Julia and Messerschmidt, S. K. and Kontermann, Roland E.},
biburl = {https://puma.ub.uni-stuttgart.de/bibtex/22fdb6559a486a8cab7aafef380d54284/cristiano},
doi = {10.1007/978-1-60761-609-2_20},
edition = {2010/03/11},
interhash = {dad7a652234bef5287e8541878210137},
intrahash = {2fdb6559a486a8cab7aafef380d54284},
isbn = {978-1-60761-608-5},
issn = {19406029},
journal = {Methods in molecular biology (Clifton, N.J.)},
keywords = {2010 izi kontermann},
pages = {295--308},
pmid = {20217604},
timestamp = {2018-07-25T12:32:43.000+0200},
title = {{Antibody targeting of nanoparticles to tumor-specific receptors: immunoliposomes.}},
url = {https://www.ncbi.nlm.nih.gov/pubmed/20217604},
volume = 624,
year = 2010
}
