%0 Journal Article %1 D0SM02043E %A Geisler, Ramsia %A Pedersen, Martin Cramer %A Preisig, Natalie %A Hannappel, Yvonne %A Prévost, Sylvain %A Dattani, Rajeev %A Arleth, Lise %A Hellweg, Thomas %D 2021 %I The Royal Society of Chemistry %J Soft Matter %K myown %N 7 %P 1888-1900 %R 10.1039/D0SM02043E %T Aescin – a natural soap for the formation of lipid nanodiscs with tunable size %U http://dx.doi.org/10.1039/D0SM02043E %V 17 %X The saponin β-aescin from the seed extract of the horse chestnut tree Aesculus hippocastanum has demonstrated a beneficial role in clinical therapy which is in part related to its strong interaction with biological membranes. In this context the present work investigates the self-assembly of nm-sized discoidal lipid nanoparticles composed of β-aescin and the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC). The discoidal lipid nanoparticles reassemble from small discs into larger discs, ribbons and finally stacks of sheets upon heating from gel-phase to fluid phase DMPC. The morphological transition of the lipid nano-particles is mainly triggered by the phospholipid phase state change. The final morphology depends on the phospholipid-to-saponin ratio and the actual temperature. The study is conducted by small-angle X-ray scattering (SAXS) and transmission (TEM) and freeze fracture electron microscopy (FFEM) are used to cover larger length scales. Two different models, representing a disc and ribbon-like shape are applied to the SAXS data, evaluating possible geometries and molecular mixing of the nano-particles. The stacked sheets are analysed by the Caillé theory.

@article{D0SM02043E, abstract = {The saponin β-aescin from the seed extract of the horse chestnut tree Aesculus hippocastanum has demonstrated a beneficial role in clinical therapy which is in part related to its strong interaction with biological membranes. In this context the present work investigates the self-assembly of nm-sized discoidal lipid nanoparticles composed of β-aescin and the phospholipid 1{,}2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC). The discoidal lipid nanoparticles reassemble from small discs into larger discs{,} ribbons and finally stacks of sheets upon heating from gel-phase to fluid phase DMPC. The morphological transition of the lipid nano-particles is mainly triggered by the phospholipid phase state change. The final morphology depends on the phospholipid-to-saponin ratio and the actual temperature. The study is conducted by small-angle X-ray scattering (SAXS) and transmission (TEM) and freeze fracture electron microscopy (FFEM) are used to cover larger length scales. Two different models{,} representing a disc and ribbon-like shape are applied to the SAXS data{,} evaluating possible geometries and molecular mixing of the nano-particles. The stacked sheets are analysed by the Caillé theory.}, added-at = {2021-03-23T14:49:08.000+0100}, author = {Geisler, Ramsia and Pedersen, Martin Cramer and Preisig, Natalie and Hannappel, Yvonne and Prévost, Sylvain and Dattani, Rajeev and Arleth, Lise and Hellweg, Thomas}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/22e6704c8be52fe33a6888f650c1a5391/nataliepreisig}, description = {Aescin – a natural soap for the formation of lipid nanodiscs with tunable size - Soft Matter (RSC Publishing)}, doi = {10.1039/D0SM02043E}, interhash = {ec71a77a19b56e842d1feb2bdea57a14}, intrahash = {2e6704c8be52fe33a6888f650c1a5391}, journal = {Soft Matter}, keywords = {myown}, number = 7, pages = {1888-1900}, publisher = {The Royal Society of Chemistry}, timestamp = {2021-04-16T12:31:19.000+0200}, title = {Aescin – a natural soap for the formation of lipid nanodiscs with tunable size}, url = {http://dx.doi.org/10.1039/D0SM02043E}, volume = 17, year = 2021 }