%0 Journal Article %1 hoffart_high_2017 %A Hoffart, Eugenia %A Grenz, Sebastian %A Lange, Julian %A Nitschel, Robert %A Müller, Felix %A Schwentner, Andreas %A Feith, André %A Lenfers-Lücker, Mira %A Takors, Ralf %A Blombach, Bastian %D 2017 %J Appl. Environ. Microbiol. %K imported myown %R 10.1128/AEM.01614-17 %T High substrate uptake rates empower Vibrio natriegens as production host for industrial biotechnology %X The productivity of industrial fermentation processes is essentially limited by the biomass specific substrate consumption rate (qS) of the applied microbial production system. Since qS depends on the growth rate (μ), we highlight the potential of the fastest growing non-pathogenic bacterium, Vibrio natriegens, as novel candidate for future biotechnological processes. V. natriegens grows rapidly in BHIN complex medium with a μ of up to 4.43 h-1 (doubling time of 9.4 min) as well as in minimal medium supplemented with various industrially relevant substrates. Bioreactor cultivations in minimal medium with glucose showed that V. natriegens possesses an exceptionally high qS under aerobic (3.90 ± 0.08 g g-1 h-1) and anaerobic (7.81 ± 0.71 g g-1 h-1) conditions. Fermentations with resting cells of genetically engineered V. natriegens under anaerobic conditions yielded an overall volumetric productivity of 0.56 ± 0.10 g alanine L-1 min-1 (i.e. 34 g L-1 h-1). These inherent properties render V. natriegens a promising new microbial platform for future industrial fermentation processes operating with high productivity.Importance Low conversion rates are one major challenge to realize microbial fermentation processes for the production of commodities operating competitively to existing petrochemical approaches. For this reason, we screened for a novel platform organism possessing superior characteristics to traditionally employed microbial systems. We identified the fast growing Vibrio natriegens which exhibits a versatile metabolism and shows striking growth and conversion rates, as a solid candidate to reach outstanding productivities. Due to these inherent characteristics V. natriegens can speed up common laboratory routines, is suitable for already existing production procedures, and forms an excellent foundation to engineer next generation bioprocesses.

@article{hoffart_high_2017, abstract = {The productivity of industrial fermentation processes is essentially limited by the biomass specific substrate consumption rate (qS) of the applied microbial production system. Since qS depends on the growth rate (μ), we highlight the potential of the fastest growing non-pathogenic bacterium, Vibrio natriegens, as novel candidate for future biotechnological processes. V. natriegens grows rapidly in BHIN complex medium with a μ of up to 4.43 h-1 (doubling time of 9.4 min) as well as in minimal medium supplemented with various industrially relevant substrates. Bioreactor cultivations in minimal medium with glucose showed that V. natriegens possesses an exceptionally high qS under aerobic (3.90 ± 0.08 g g-1 h-1) and anaerobic (7.81 ± 0.71 g g-1 h-1) conditions. Fermentations with resting cells of genetically engineered V. natriegens under anaerobic conditions yielded an overall volumetric productivity of 0.56 ± 0.10 g alanine L-1 min-1 (i.e. 34 g L-1 h-1). These inherent properties render V. natriegens a promising new microbial platform for future industrial fermentation processes operating with high productivity.Importance Low conversion rates are one major challenge to realize microbial fermentation processes for the production of commodities operating competitively to existing petrochemical approaches. For this reason, we screened for a novel platform organism possessing superior characteristics to traditionally employed microbial systems. We identified the fast growing Vibrio natriegens which exhibits a versatile metabolism and shows striking growth and conversion rates, as a solid candidate to reach outstanding productivities. Due to these inherent characteristics V. natriegens can speed up common laboratory routines, is suitable for already existing production procedures, and forms an excellent foundation to engineer next generation bioprocesses.}, added-at = {2018-02-09T13:18:17.000+0100}, author = {Hoffart, Eugenia and Grenz, Sebastian and Lange, Julian and Nitschel, Robert and Müller, Felix and Schwentner, Andreas and Feith, André and Lenfers-Lücker, Mira and Takors, Ralf and Blombach, Bastian}, biburl = {https://puma.ub.uni-stuttgart.de/bibtex/22ceca70ad0e6f4e3df9bb111052565e9/bastian}, doi = {10.1128/AEM.01614-17}, interhash = {100fdc7f1a696b417d9348712aab353f}, intrahash = {2ceca70ad0e6f4e3df9bb111052565e9}, issn = {1098-5336}, journal = {Appl. Environ. Microbiol.}, keywords = {imported myown}, language = {eng}, month = sep, pmcid = {PMC5666143}, pmid = {28887417}, timestamp = {2018-02-09T12:18:56.000+0100}, title = {High substrate uptake rates empower {Vibrio} natriegens as production host for industrial biotechnology}, year = 2017 }